CoChat
Sign Up
Cited by 23

The DOI matches its Crossref record.

Known Allergen Structures Predict Schistosoma mansoni IgE-Binding Antigens in Human Infection

Farnell, Edward J., Tyagi, Nidhi, Ryan, Stephanie, Chalmers, Iain W., Pinot de Moira, Angela, Jones, Frances M., Wawrzyniak, Jakub, Fitzsimmons, Colin M., Tukahebwa, Edridah M., Furnham, Nicholas, Maizels, Rick M., Dunne, David W.·2015·Frontiers in Immunology
DOI 10.3389/fimmu.2015.00026PMC PMC4315118
Open this paper in CoChat — free account

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3389/fimmu.2015.00026

Cites

Statistics

Figures

Figure 1 SDS-PAGE of recombinant antigens following expression and purification. Proteins were run on a 4–12% gradient Bis-Tris gel and stained with Coomassie Blue.
Figure 1 SDS-PAGE of recombinant antigens following expression and purification. Proteins were run on a 4–12% gradient Bis-Tris gel and stained with Coomassie Blue.CC BY · PubMed Central
Figure 2 Prevalence of IgE (A), IgG4 (B), and IgG1 (C) responses (with 95% binomial CIs), both pre- and post-treatment with PZQ to recombinant antigens within the population (n = 222). Prevalences/proportions of responders were calculated following the application of assay sensitivity cut-offs (mean + 3σ) determined using sera from 26 normal European controls. Proteins were grouped into predicted IgE-binding antigens (I), antigens without known allergenic domains (II), and confirmed IgE-binding antigens (III).
Figure 2 Prevalence of IgE (A), IgG4 (B), and IgG1 (C) responses (with 95% binomial CIs), both pre- and post-treatment with PZQ to recombinant antigens within the population (n = 222). Prevalences/proportions of responders were calculated following the application of assay sensitivity cut-offs (mean + 3σ) determined using sera from 26 normal European controls. Proteins were grouped into predicted IgE-binding antigens (I), antigens without known allergenic domains (II), and confirmed IgE-binding antigens (III).CC BY · PubMed Central
Figure 3 Proportions of individuals responding with IgE alone, IgG4 alone, or IgG4 and IgE together post-treatment with PZQ. Prevalences/proportions of responders were calculated following the application of assay sensitivity cut-offs (mean + 3σ) determined using sera from 26 normal European controls. Two-sample test of proportions was used to compare the prevalence of individuals responding with IgE alone vs. those responding with IgE and IgG4 together (*p < 0.05, **p < 0.01, ***p < 0.001). Proteins were grouped into predicted IgE-binding antigens (I), antigens without known allergenic domains (II), and confirmed IgE-binding antigens (III).
Figure 3 Proportions of individuals responding with IgE alone, IgG4 alone, or IgG4 and IgE together post-treatment with PZQ. Prevalences/proportions of responders were calculated following the application of assay sensitivity cut-offs (mean + 3σ) determined using sera from 26 normal European controls. Two-sample test of proportions was used to compare the prevalence of individuals responding with IgE alone vs. those responding with IgE and IgG4 together (*p < 0.05, **p < 0.01, ***p < 0.001). Proteins were grouped into predicted IgE-binding antigens (I), antigens without known allergenic domains (II), and confirmed IgE-binding antigens (III).CC BY · PubMed Central
Figure 4 Comparison of the structures of SmLipocalin, SmThioredoxin, SmSOD with their respective IgE-binding counterparts identified from Allergome. Parasite allergen-like molecules were used to identify similar allergens by BLAST search of the Allergome database. Identities of the matched allergens with the corresponding E-values are shown below each matched pair. Structures were superimposed using LSQMAN and the quality of the alignment assessed by root mean squared deviation (RMSD). (A) Alignment of SmLipocalin (1VYF, cyan) and Der f 13 (2A0A, green). (B) Alignment of SmSOD (1TO4, cyan) and Tomato SOD (3HOG, green). (C) Alignment of SmThioredoxin (2XBI, cyan) and White Shrimp Thioredoxin (3ZZX, green).
Figure 4 Comparison of the structures of SmLipocalin, SmThioredoxin, SmSOD with their respective IgE-binding counterparts identified from Allergome. Parasite allergen-like molecules were used to identify similar allergens by BLAST search of the Allergome database. Identities of the matched allergens with the corresponding E-values are shown below each matched pair. Structures were superimposed using LSQMAN and the quality of the alignment assessed by root mean squared deviation (RMSD). (A) Alignment of SmLipocalin (1VYF, cyan) and Der f 13 (2A0A, green). (B) Alignment of SmSOD (1TO4, cyan) and Tomato SOD (3HOG, green). (C) Alignment of SmThioredoxin (2XBI, cyan) and White Shrimp Thioredoxin (3ZZX, green).CC BY · PubMed Central
Figure 5 Differential transcription of antigens in S. mansoni adult worms (A) and eggs (B) as determined by previously published microarray studies (35). Levels of antigen transcription were expressed as relative fluorescent units (RFU).
Figure 5 Differential transcription of antigens in S. mansoni adult worms (A) and eggs (B) as determined by previously published microarray studies (35). Levels of antigen transcription were expressed as relative fluorescent units (RFU).CC BY · PubMed Central
Figure 6 Relationships between antigen-specific natural log normalized geometric mean (GM) IgE levels and transcription of antigen in egg (A)*, natural log normalized GM IgG4 levels (B), GM age (C), or GM infection intensity (D) in individuals that respond to either IgG4 or IgE both pre- (open circles, short dashes) and post-treatment (filled circles, long dashes) with PZQ to a given antigen (β, SE, and p value calculated by linear regression). *SmHSP20 was excluded from this plot as it exerted undue influence on the linear regression model (Cook’s distance > 1).
Figure 6 Relationships between antigen-specific natural log normalized geometric mean (GM) IgE levels and transcription of antigen in egg (A)*, natural log normalized GM IgG4 levels (B), GM age (C), or GM infection intensity (D) in individuals that respond to either IgG4 or IgE both pre- (open circles, short dashes) and post-treatment (filled circles, long dashes) with PZQ to a given antigen (β, SE, and p value calculated by linear regression). *SmHSP20 was excluded from this plot as it exerted undue influence on the linear regression model (Cook’s distance > 1).CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 54

  1. Mari, 2006, Bioinformatics applied to allergy: allergen databases, from collecting sequence information to data integration. The Allergome platform as a model, Cell Immunol, 244, 97, 10.1016/j.cellimm.2007.02.012 · DOI
  2. Traidl-Hoffmann, 2009, Determinants of allergenicity, J Allergy Clin Immunol, 123, 558, 10.1016/j.jaci.2008.12.003 · DOI
  3. Radauer, 2008, Allergens are distributed into few protein families and possess a restricted number of biochemical functions, J Allergy Clin Immunol, 121, 847–52.e7, 10.1016/j.jaci.2008.01.025 · DOI · Paper page
  4. Palm, 2012, Allergic host defences, Nature, 484, 465, 10.1038/nature11047 · DOI · Paper page
  5. Fitzsimmons, 2009, Survival of the fittest: allergology or parasitology?, Trends Parasitol, 25, 447, 10.1016/j.pt.2009.07.004 · DOI · Paper page
  6. Faulkner, 2002, Age- and infection intensity-dependent cytokine and antibody production in human trichuriasis: the importance of IgE, J Infect Dis, 185, 665, 10.1086/339005 · DOI
  7. Turner, 2005, Allergen-specific IgE and IgG4 are markers of resistance and susceptibility in a human intestinal nematode infection, Microbes Infect, 7, 990, 10.1016/j.micinf.2005.03.036 · DOI
  8. Rihet, 1991, Evidence for an association between human resistance to Schistosoma mansoni and high anti-larval IgE levels, Eur J Immunol, 21, 2679, 10.1002/eji.1830211106 · DOI
  9. Fitzsimmons, 2014, Helminth allergens, parasite-specific ige, and its protective role in human immunity, Front Immunol, 5, 61, 10.3389/fimmu.2014.00061 · DOI · Paper page
  10. Dunne, 1992, Human IgE responses to Schistosoma mansoni and resistance to reinfection, Mem Inst Oswaldo Cruz, 87, 99, 10.1590/S0074-02761992000800014 · DOI
  11. Hagan, 1991, Human IgE, IgG4 and resistance to reinfection with Schistosoma haematobium, Nature, 349, 243, 10.1038/349243a0 · DOI
  12. Pritchard, 1995, Immunity in humans to Necator americanus: IgE, parasite weight and fecundity, Parasite Immunol, 17, 71, 10.1111/j.1365-3024.1995.tb00968.x · DOI
  13. Matsuda, 1990, Necessity of IgE antibodies and mast cells for manifestation of resistance against larval Haemaphysalis longicornis ticks in mice, J Immunol, 144, 259, 10.4049/jimmunol.144.1.259 · DOI · Paper page
  14. Jenkins, 2007, Evolutionary distance from human homologs reflects allergenicity of animal food proteins, J Allergy Clin Immunol, 120, 1399, 10.1016/j.jaci.2007.08.019 · DOI
  15. Fitzsimmons, 2007, Factors affecting human IgE and IgG responses to allergen-like Schistosoma mansoni antigens: molecular structure and patterns of in vivo exposure, Int Arch Allergy Immunol, 142, 40, 10.1159/000095997 · DOI
  16. Jenkins, 1998, Tropomyosin implicated in host protective responses to microfilariae in onchocerciasis, Proc Natl Acad Sci U S A, 95, 7550, 10.1073/pnas.95.13.7550 · DOI
  17. Silas, 2014, Human IgE responses to different splice variants of Schistosoma mansoni tropomyosin: associations with immunity, Int J Parasitol, 44, 381, 10.1016/j.ijpara.2014.02.004 · DOI
  18. Diemert, 2012, Generalized urticaria induced by the Na-ASP-2 hookworm vaccine: implications for the development of vaccines against helminths, J Allergy Clin Immunol, 130, 169–76.e6, 10.1016/j.jaci.2012.04.027 · DOI
  19. Gaze, 2014, An immunomics approach to schistosome antigen discovery: antibody signatures of naturally resistant and chronically infected individuals from endemic areas, PLoS Pathog, 10, e1004033, 10.1371/journal.ppat.1004033 · DOI
  20. Braschi, 2006, The tegument surface membranes of the human blood parasite Schistosoma mansoni: a proteomic analysis after differential extraction, Proteomics, 6, 1471, 10.1002/pmic.200500368 · DOI
  21. Curwen, 2004, The Schistosoma mansoni soluble proteome: a comparison across four life-cycle stages, Mol Biochem Parasitol, 138, 57, 10.1016/j.molbiopara.2004.06.016 · DOI
  22. Mutapi, 2011, Differential recognition patterns of Schistosoma haematobium adult worm antigens by the human antibodies IgA, IgE, IgG1 and IgG4, Parasite Immunol, 33, 181, 10.1111/j.1365-3024.2010.01270.x · DOI
  23. Maizels, 2003, Immune regulation by helminth parasites: cellular and molecular mechanisms, Nat Rev Immunol, 3, 733, 10.1038/nri1183 · DOI
  24. Fitzsimmons, 2012, The Schistosoma mansoni tegumental-allergen-like (TAL) protein family: influence of developmental expression on human IgE responses, PLoS Negl Trop Dis, 6, e1593, 10.1371/journal.pntd.0001593 · DOI
  25. Akdis, 2011, Mechanisms of allergen-specific immunotherapy, J Allergy Clin Immunol, 127, 18, 10.1016/j.jaci.2010.11.030 · DOI
  26. Jutel, 2005, Allergen-specific immunotherapy with recombinant grass pollen allergens, J Allergy Clin Immunol, 116, 608, 10.1016/j.jaci.2005.06.004 · DOI
  27. Colley, 2014, Human schistosomiasis, Lancet, 383, 2253, 10.1016/S0140-6736(13)61949-2 · DOI
  28. Wilson, 2009, Immune effector mechanisms against schistosomiasis: looking for a chink in the parasite’s armour, Trends Parasitol, 25, 423, 10.1016/j.pt.2009.05.011 · DOI
  29. Pinot de Moira, 2013, Schistosoma mansoni infection in preschool-aged children: development of immunoglobulin E and immunoglobulin G4 responses to parasite allergen-like proteins, J Infect Dis, 207, 362, 10.1093/infdis/jis676 · DOI
  30. Hoffmann, 2004, Gene expression studies using self-fabricated parasite cDNA microarrays, Methods Mol Biol, 270, 219, 10.1385/1-59259-793-9:219 · DOI
  31. UniProt Consortium, 2013, Update on activities at the Universal Protein Resource (UniProt) in 2013, Nucleic Acids Res, 41, D43, 10.1093/nar/gks1068 · DOI
  32. Å ali, 1993, Comparative protein modelling by satisfaction of spatial restraints, J Mol Biol, 234, 779, 10.1006/jmbi.1993.1626 · DOI · Paper page
  33. Zinsser, 2013, Triose phosphate isomerase from the blood fluke Schistosoma mansoni: biochemical characterisation of a potential drug and vaccine target, FEBS Lett, 587, 3422, 10.1016/j.febslet.2013.09.022 · DOI
  34. Cardoso, 2001, Expression and preliminary X-ray diffraction studies of cytosolic Cu,Zn superoxide dismutase from Schistosoma mansoni, Acta Crystallogr D Biol Crystallogr, 57, 1877, 10.1107/S0907444901014500 · DOI
  35. Fitzpatrick, 2009, Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses, PLoS Negl Trop Dis, 3, e543, 10.1371/journal.pntd.0000543 · DOI
  36. Pinot de Moira, 2010, Analysis of complex patterns of human exposure and immunity to Schistosomiasis mansoni: the influence of age, sex, ethnicity and IgE, PLoS Negl Trop Dis, 4, e820, 10.1371/journal.pntd.0000820 · DOI
  37. Pinot de Moira, 2013, Effects of treatment on IgE responses against parasite allergen-like proteins and immunity to reinfection in childhood schistosome and hookworm coinfections, Infect Immun, 81, 23, 10.1128/IAI.00748-12 · DOI · Paper page
  38. Fitzsimmons, 2012, Progressive cross-reactivity in IgE responses: an explanation for the slow development of human immunity to schistosomiasis?, Infect Immun, 80, 4264, 10.1128/IAI.00641-12 · DOI
  39. Farias, 2012, Schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation, PLoS Negl Trop Dis, 6, e1510, 10.1371/journal.pntd.0001510 · DOI
  40. Ortona, 2002, Immunological characterization of Echinococcus granulosus cyclophilin, an allergen reactive with IgE and IgG4 from patients with cystic echinococcosis, Clin Exp Immunol, 128, 124, 10.1046/j.1365-2249.2002.01807.x · DOI · Paper page
  41. Ishiguro, 1992, Identification of Candida albicans antigens reactive with immunoglobulin E antibody of human sera, Infect Immun, 60, 1550, 10.1128/IAI.60.4.1550-1557.1992 · DOI
  42. Fonseca, 2014, Maize IgE binding proteins: each plant a different profile?, Proteome Sci, 12, 17, 10.1186/1477-5956-12-17 · DOI
  43. Fonseca, 2012, Characterization of maize allergens – MON810 vs. its non-transgenic counterpart, J Proteomics, 75, 2027, 10.1016/j.jprot.2012.01.005 · DOI
  44. Chapman, 2007, Nomenclature and structural biology of allergens, J Allergy Clin Immunol, 119, 414, 10.1016/j.jaci.2006.11.001 · DOI
  45. Fujita, 2012, Mechanisms of allergen-specific immunotherapy, Clin Transl Allergy, 2, 2, 10.1186/2045-7022-2-2 · DOI
  46. Erler, 2011, Proteomic profiling of birch (Betula verrucosa) pollen extracts from different origins, Proteomics, 11, 1486, 10.1002/pmic.201000624 · DOI
  47. Goldstein, 1975, Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells, Proc Natl Acad Sci U S A, 72, 11, 10.1073/pnas.72.1.11 · DOI
  48. Carvalho-Queiroz, 2004, Cross-reactivity of Schistosoma mansoni cytosolic superoxide dismutase, a protective vaccine candidate, with host superoxide dismutase and identification of parasite-specific B epitopes, Infect Immun, 72, 2635, 10.1128/IAI.72.5.2635-2647.2004 · DOI
  49. Lee, 1995, Immune response to Schistosoma mansoni phosphoglycerate kinase during natural and experimental infection: identification of a schistosome-specific B-cell epitope, Infect Immun, 63, 4307, 10.1128/IAI.63.11.4307-4311.1995 · DOI
  50. Brito, 2000, Human IgG1 and IgG3 recognition of Schistosoma mansoni 14kDa fatty acid-binding recombinant protein, Parasite Immunol, 22, 41
  51. Schechtman, 2001, The 14-3-3 protein as a vaccine candidate against schistosomiasis, Parasite Immunol, 23, 213, 10.1046/j.1365-3024.2001.00378.x · DOI
  52. Capron, 2002, Vaccine development against schistosomiasis from concepts to clinical trials, Br Med Bull, 62, 139, 10.1093/bmb/62.1.139 · DOI
  53. Gleich, 1982, Effect of immunotherapy on immunoglobulin E and immunoglobulin G antibodies to ragweed antigens: a six-year prospective study, J Allergy Clin Immunol, 70, 261, 10.1016/0091-6749(82)90062-8 · DOI
  54. Pontes-de-Carvalho, 2014, Question of nature: some antigens are bound to be allergens, Front Immunol, 5, 373, 10.3389/fimmu.2014.00373 · DOI

Cited by

Sorted by citations

See all 23

Metadata

Authors
Farnell, E. J., Tyagi, N., Ryan, S., Chalmers, I. W., Pinot de Moira, A., Jones, F. M., Wawrzyniak, J., Fitzsimmons, C. M., Tukahebwa, E. M., Furnham, N., Maizels, R. M., & Dunne, D. W.
Venue
Frontiers in Immunology
Publisher
Frontiers Media SA
Published
2015-02-03
Volume
6
ISSN
1664-3224
DOI
10.3389/fimmu.2015.00026
PMC
PMC4315118
Cited by
23
References
0

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Known Allergen Structures Predict Schistosoma mansoni IgE-Binding Antigens in Human Infection. https://app.cochat.ai/papers/10.3389/fimmu.2015.00026
The paper
Farnell, E. J., Tyagi, N., Ryan, S., Chalmers, I. W., Pinot de Moira, A., Jones, F. M., Wawrzyniak, J., Fitzsimmons, C. M., Tukahebwa, E. M., Furnham, N., Maizels, R. M., & Dunne, D. W. (2015). Known Allergen Structures Predict Schistosoma mansoni IgE-Binding Antigens in Human Infection. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2015.00026