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The Microbiome of Potentially Malignant Oral Leukoplakia Exhibits Enrichment for Fusobacterium, Leptotrichia, Campylobacter, and Rothia Species

Amer, Abdrazak, Galvin, Sheila, Healy, Claire M., Moran, Gary P.·2017·Frontiers in Microbiology
DOI 10.3389/fmicb.2017.02391PMC PMC5717034
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FIGURE 1 Overview of bacterial phyla distribution and Candida carriage patterns in patients with OLK and healthy controls. (A) Graph showing the abundance of the six most important phyla in healthy control subjects and sites of OLK and contralateral normal sites in patients. ∗ Indicates Metastats P < 0.05 and ∗∗P < 0.01. (B) Percentage of Candida positive samples recovered from patients (OLK and contralateral sites) and healthy controls, determined by qPCR.
FIGURE 1 Overview of bacterial phyla distribution and Candida carriage patterns in patients with OLK and healthy controls. (A) Graph showing the abundance of the six most important phyla in healthy control subjects and sites of OLK and contralateral normal sites in patients. ∗ Indicates Metastats P < 0.05 and ∗∗P < 0.01. (B) Percentage of Candida positive samples recovered from patients (OLK and contralateral sites) and healthy controls, determined by qPCR.CC BY · PubMed Central
FIGURE 2 Analysis community structure in samples from OLK and healthy mucosa. (A) Rarefaction curve showing analysis of OTUs identified in samples from healthy controls (green) and patients (red = OLK tissue, orange = contralateral normal tissue). (B) Non-metric multidimensional scaling (NMDS) plots generated from a matrix of Bray–Curtis dissimilarity values using Mothur (Schloss et al., 2009). Separation of OLK communities (red) from contralateral (orange; P = 0.007) and healthy control communities (green; P = 0.001) was determined using analysis of molecular variance (AMOVA).
FIGURE 2 Analysis community structure in samples from OLK and healthy mucosa. (A) Rarefaction curve showing analysis of OTUs identified in samples from healthy controls (green) and patients (red = OLK tissue, orange = contralateral normal tissue). (B) Non-metric multidimensional scaling (NMDS) plots generated from a matrix of Bray–Curtis dissimilarity values using Mothur (Schloss et al., 2009). Separation of OLK communities (red) from contralateral (orange; P = 0.007) and healthy control communities (green; P = 0.001) was determined using analysis of molecular variance (AMOVA).CC BY · PubMed Central
FIGURE 3 Results of LEfSe analysis to identify significantly enriched bacterial taxa in (A) communities from patients and healthy controls and (B) in communities from sites of OLK and contralateral tissue from patients.
FIGURE 3 Results of LEfSe analysis to identify significantly enriched bacterial taxa in (A) communities from patients and healthy controls and (B) in communities from sites of OLK and contralateral tissue from patients.CC BY · PubMed Central
FIGURE 4 Relative abundance of selected taxonomic groups identified by LEfSe analysis. The abundance of each organism (A–F) in OLK and contralateral healthy communities from each patient is plotted side by side. No healthy tissues were available from four patients (Supplementary Table S1) and these are plotted versus the average values for control tissue. P-values refer to Wilcoxon matched pairs test results.
FIGURE 4 Relative abundance of selected taxonomic groups identified by LEfSe analysis. The abundance of each organism (A–F) in OLK and contralateral healthy communities from each patient is plotted side by side. No healthy tissues were available from four patients (Supplementary Table S1) and these are plotted versus the average values for control tissue. P-values refer to Wilcoxon matched pairs test results.CC BY · PubMed Central
FIGURE 5 (A) Heatmap showing the abundance of taxa identified by LEfSe analysis. Heatmap and dendogram were generated in Vegan using a matrix of Bray–Curtis dissimilarity values. Separation of patient clusters marked 1 to 5 in the dendogram was highly significant (Unweighted UNIFRAC P < 0.001). The color-coded legend indicates whether each sample is from a buccal or lingual site and the degree of dysplasia following biopsy (mild, moderate, or severe). CON (light blue) correspond to the average values in healthy buccal (left) and healthy lingual (right) samples. (B) Co-occurrence map generated from Pearson correlation coefficients (r) generated in Prism (P < 0.01) and graphically presented using Cytoscape 3.2.1. Thickness of edges (connecting lines) are proportional to r-values (0.4–0.99). Clusters within the dotted line were identified in patients who were also colonized with Candida spp.
FIGURE 5 (A) Heatmap showing the abundance of taxa identified by LEfSe analysis. Heatmap and dendogram were generated in Vegan using a matrix of Bray–Curtis dissimilarity values. Separation of patient clusters marked 1 to 5 in the dendogram was highly significant (Unweighted UNIFRAC P < 0.001). The color-coded legend indicates whether each sample is from a buccal or lingual site and the degree of dysplasia following biopsy (mild, moderate, or severe). CON (light blue) correspond to the average values in healthy buccal (left) and healthy lingual (right) samples. (B) Co-occurrence map generated from Pearson correlation coefficients (r) generated in Prism (P < 0.01) and graphically presented using Cytoscape 3.2.1. Thickness of edges (connecting lines) are proportional to r-values (0.4–0.99). Clusters within the dotted line were identified in patients who were also colonized with Candida spp.CC BY · PubMed Central

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Metadata

Authors
Amer, A., Galvin, S., Healy, C. M., & Moran, G. P.
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2017-12-01
Volume
8
ISSN
1664-302X
DOI
10.3389/fmicb.2017.02391
PMC
PMC5717034
Cited by
160
References
3
Funders
Ministry of Education

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CoChat. (2026). Citation check: The Microbiome of Potentially Malignant Oral Leukoplakia Exhibits Enrichment for Fusobacterium, Leptotrichia, Campylobacter, and Rothia Species. https://app.cochat.ai/papers/10.3389/fmicb.2017.02391
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Amer, A., Galvin, S., Healy, C. M., & Moran, G. P. (2017). The Microbiome of Potentially Malignant Oral Leukoplakia Exhibits Enrichment for Fusobacterium, Leptotrichia, Campylobacter, and Rothia Species. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2017.02391