The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
Role of Tryptophan in Microbiota-Induced Depressive-Like Behavior: Evidence From Tryptophan Depletion Study
Open accessAbstract
During the past decade, there has been a substantial rise in the knowledge about the effects of gut microbiota on host physiology and behavior, including depressive behavior. Initial studies determined that gut microbiota can regulate host tryptophan levels, which is a main serotonin precursor. A dysfunctional serotonergic system is considered to be one of the main factors contributing to the development of depression. Therefore, we hypothesized that regulation of brain tryptophan and serotonin can explain, at least partly, the effects of microbiota on depressive behavior. To test this hypothesis, we examined depressive-like behavior and brain levels of serotonin and tryptophan, of germ free (GF) and specific-pathogen free (SPF) mice under basal conditions, or after acute tryptophan depletion (ATD) procedure, which is a method to decrease tryptophan and serotonin levels in the brain. In basal conditions, GF mice exhibited less depressive-like behavior in sucrose preference, tail-suspension and forced swim tests, compared to SPF mice. In addition, in mice that were not subjected to ATD, GF mice displayed higher levels of tryptophan, serotonin and 5-hydroxyindoleacetic acid (the main degradation product of serotonin) in medial prefrontal cortex (mPFC) and hippocampus (HIPPO), compared to SPF mice. Interestingly, ATD increased depressive-like behavior of GF, but not of SPF mice. These behavioral changes were accompanied by a stronger reduction of tryptophan, serotonin and 5-hydroxyindoleacetic acid in mPFC and HIPPO in GF mice after ATD, when compared to SPF mice. Therefore, the serotonergic system of GF mice is more vulnerable to the acute challenge of tryptophan reduction, and GF mice after tryptophan reduction behave more similarly to SPF mice. These data provide functional evidence that microbiota affects depression-like behavior through influencing brain tryptophan accessibility and the serotonergic system.
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“These changes include increased hippocampal dopamine D1 receptor mRNA levels and decreased dopamine D1 receptor in the striatum and nucleus accumbens, as well as increased serotonin in the blood and hippocampus, with decreased serotonin receptor expression, respectively.”
From Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis (2023) · cites this paper as [378]
In the citing paper
“Several metabolomic studies show alterations in the dopamine and serotonin systems (124, 189, 350, 378–380).”
From Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis (2023) · cites this paper as [378]
In the citing paper
“Additionally, the production of 5-HT can affect peripheral tryptophan availability and, thus, indirectly affect brain 5-HT levels, suggesting that peripheral alterations in 5-HT can affect the central 5-HT signaling system (390, 391).”
From Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health (2021) · cites this paper as [391]
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Indigenous bacteria from the gut microbiota regulate host serotonin biosynthesis
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Gut Microbiota Regulation of Tryptophan Metabolism in Health and Disease
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“TRP, which is an essential amino acid, is mainly supplied from ingested food and is a biosynthetic precursor of a large number of host as well as microbial metabolites (Milligan et al., 1978; Lee and Lee, 2010; Agus et al., 2018).”
BackgroundSudo, N., Chida, Y., Aiba, Y., Sonoda, J., Oyama, N., Yu, X., Kubo, C., & Koga, Y. (2004) · The Journal of Physiology · doi:10.1113/jphysiol.2004.063388 · Cited by 2,735
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Research works printed but not linked: 1
- Yamaguchi, 1981, Demonstration of tryptophan 5-monooxygenase activity in human-brain by highly sensitive high-performance liquid-chromatography with fluorometric detection, Biochem. Int., 2, 295
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Items 101–102 of 102
Kim, S.-H., Hong, S., Lee, E. Y., Kim, S.-A., Han, N. S., Kim, S. Y., & Lee, C. H. (2026) · Gut Microbes · doi:10.1080/19490976.2026.2728833
Tryptophan metabolism: a critical bridge linking gut microbiota to depression
Tan, H., Pang, Y., Ji, Q., Li, Z., Liang, J., Xian, R., Zhou, P., & Zhang, Y. (2026) · Frontiers in Microbiology · doi:10.3389/fmicb.2026.1936353
Metadata
- Authors
- Lukić, I., Getselter, D., Koren, O., & Elliott, E.
- Affiliations
- Bar-Ilan University (IL)
- Corresponding author
- Elliott, Evan
- Venue
- Frontiers in Behavioral Neuroscience
- Publisher
- Frontiers Media SA
- Published
- 2019-06-04
- Volume
- 13
- Pages
- 123-123
- ISSN
- 1662-5153 (ISSN-L)
- PMC
- PMC6558209
- OpenAlex
- W2948144072
- Cited by
- 102
- References
- 59
- Citations by year
- 2026: 4 · 2025: 11 · 2024: 29 · 2023: 16 · 2022: 15 · 2021: 12 · 2020: 11 · 2019: 4
- FWCI
- 3.44
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Behavioral Neuroscience (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Israel Science Foundation
- Teva Pharmaceutical Industries
- Topics
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- gut microbiota, depression-like behavior, tryptophan, serotonin, acute tryptophan depletion, germ-free mice, serotonergic system, 5-hydroxyindoleacetic acid, medial prefrontal cortex, hippocampus, depression
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