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miRNA-7a-2-3p Inhibits Neuronal Apoptosis in Oxygen-Glucose Deprivation (OGD) Model

Zhang, Zi-Bin, Tan, Ya-Xin, Zhao, Qiong, Xiong, Liu-Lin, Liu, Jia, Xu, Fei-Fei, Xu, Yang, Bobrovskaya, Larisa, Zhou, Xin-Fu, Wang, Ting-Hua·2019·Frontiers in Neuroscience
DOI 10.3389/fnins.2019.00016PMC PMC6351497
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FIGURE 1 The levels of MiR-7a-2-3p are Decreased in Cerebral Cortex after HIBD in vivo. (A) The Zea Longa scores were statistically different at 0 time point, 6, 12, 24, and 48 h after HI compared with the sham group (∗∗P < 0.01). (B) MRI showed the cerebral ischemia infraction at 1 month after HI. The white arrow shows the area of cerebral ischemia infraction. (C) The expression of miR-7a-2-3p was decreased at 12, 48, and 96 h after HI (∗∗P < 0.01).
FIGURE 1 The levels of MiR-7a-2-3p are Decreased in Cerebral Cortex after HIBD in vivo. (A) The Zea Longa scores were statistically different at 0 time point, 6, 12, 24, and 48 h after HI compared with the sham group (∗∗P < 0.01). (B) MRI showed the cerebral ischemia infraction at 1 month after HI. The white arrow shows the area of cerebral ischemia infraction. (C) The expression of miR-7a-2-3p was decreased at 12, 48, and 96 h after HI (∗∗P < 0.01).CC BY · PubMed Central
FIGURE 2 The levels of MiR-7a-2-3p are Decreased in PC12 cells, SH-SY5Y Cells and Primary Cortical Neurons after OGD in vitro. (A) The images of PC12 cells, SH-SY5Y cells, and neurons after OGD are shown. Bar, 50 μm. (B) miR-7a-2-3p was downregulated significantly in PC12 cells after OGD injury when assessed by PCR (∗∗P < 0.01). (C) miR-7a-2-3p was downregulated significantly in SH-SY5Y cells after OGD when assessed by PCR (∗∗P < 0.01). (D) miR-7a-2-3p was downregulated significantly in neurons after OGD when assessed by PCR (∗∗P < 0.01).
FIGURE 2 The levels of MiR-7a-2-3p are Decreased in PC12 cells, SH-SY5Y Cells and Primary Cortical Neurons after OGD in vitro. (A) The images of PC12 cells, SH-SY5Y cells, and neurons after OGD are shown. Bar, 50 μm. (B) miR-7a-2-3p was downregulated significantly in PC12 cells after OGD injury when assessed by PCR (∗∗P < 0.01). (C) miR-7a-2-3p was downregulated significantly in SH-SY5Y cells after OGD when assessed by PCR (∗∗P < 0.01). (D) miR-7a-2-3p was downregulated significantly in neurons after OGD when assessed by PCR (∗∗P < 0.01).CC BY · PubMed Central
FIGURE 3 Overexpression of MiR-7a-2-3p Improved the morphology and increased the Number of PC12 cells, SH-SY5Y Cells and primary cortical Neurons after OGD injury. (A) The morphological changes of PC12 cells, SH-SY5Y cells and neurons are shown for the normal group, OGD group, OGD+ miR-NC group and OGD+ miR-7a-2-3p group. Bar, 50 μm. (B) The number of PC12 cells in OGD+ miR-7a-2-3p group significantly increased compared with the OGD group (∗∗P < 0.01). (C) The number of SH-SY5Y cells in OGD+ miR-7a-2-3p group significantly increased compared with the OGD group (∗∗P < 0.01). (D) The number of neurons in OGD+ miR-7a-2-3p group significant increased compared with the OGD group (∗∗P < 0.01).
FIGURE 3 Overexpression of MiR-7a-2-3p Improved the morphology and increased the Number of PC12 cells, SH-SY5Y Cells and primary cortical Neurons after OGD injury. (A) The morphological changes of PC12 cells, SH-SY5Y cells and neurons are shown for the normal group, OGD group, OGD+ miR-NC group and OGD+ miR-7a-2-3p group. Bar, 50 μm. (B) The number of PC12 cells in OGD+ miR-7a-2-3p group significantly increased compared with the OGD group (∗∗P < 0.01). (C) The number of SH-SY5Y cells in OGD+ miR-7a-2-3p group significantly increased compared with the OGD group (∗∗P < 0.01). (D) The number of neurons in OGD+ miR-7a-2-3p group significant increased compared with the OGD group (∗∗P < 0.01).CC BY · PubMed Central
FIGURE 4 Overexpression of MiR-7a-2-3p Ameliorated Neurodegeneration and improved the Growth of Neuronal Axons. (A) Tuj1+ neurons (green) are shown for the normal group, OGD group, OGD+miR-NC group and OGD+miR-7a-2-3p group. Nuclei were visualized by DAPI (blue). Bar, 50 μm. (B) The number of Tuj1+ cells was increased in OGD+miR-7a-2-3p group compared with the OGD+miR-NC (∗∗P < 0.01). (C) The axonal length of neurons was increased in the OGD+miR-7a-2-3p group compared with the OGD+miR-NC group (∗∗P < 0.01).
FIGURE 4 Overexpression of MiR-7a-2-3p Ameliorated Neurodegeneration and improved the Growth of Neuronal Axons. (A) Tuj1+ neurons (green) are shown for the normal group, OGD group, OGD+miR-NC group and OGD+miR-7a-2-3p group. Nuclei were visualized by DAPI (blue). Bar, 50 μm. (B) The number of Tuj1+ cells was increased in OGD+miR-7a-2-3p group compared with the OGD+miR-NC (∗∗P < 0.01). (C) The axonal length of neurons was increased in the OGD+miR-7a-2-3p group compared with the OGD+miR-NC group (∗∗P < 0.01).CC BY · PubMed Central
FIGURE 5 Overexpression of MiR-7a-2-3p Reduced the Apoptosis of Neurons after OGD injury. (A) The apoptotic changes in neurons (red) are shown for the normal group, OGD group, OGD+miR-NC group and OGD+ miR-7a-2-3p group. Nuclei were visualized by DAPI (blue). Bar, 50 μm. (B) The apoptotic ratio of neurons significantly increased in the OGD group compared with the normal group. When compared with the OGD+miR-NC group, the apoptotic ratio of neurons significantly decreased in the OGD+miR-7a-2-3p group (∗∗P < 0.01). (C) The viability of neurons was quantified by MTT. The viability of neurons was markedly decreased in the OGD group compared with the normal group. When compared with the OGD+miR-NC, the viability of neurons significantly increased in the OGD + miR-7a-2-3p group (∗∗P < 0.01).
FIGURE 5 Overexpression of MiR-7a-2-3p Reduced the Apoptosis of Neurons after OGD injury. (A) The apoptotic changes in neurons (red) are shown for the normal group, OGD group, OGD+miR-NC group and OGD+ miR-7a-2-3p group. Nuclei were visualized by DAPI (blue). Bar, 50 μm. (B) The apoptotic ratio of neurons significantly increased in the OGD group compared with the normal group. When compared with the OGD+miR-NC group, the apoptotic ratio of neurons significantly decreased in the OGD+miR-7a-2-3p group (∗∗P < 0.01). (C) The viability of neurons was quantified by MTT. The viability of neurons was markedly decreased in the OGD group compared with the normal group. When compared with the OGD+miR-NC, the viability of neurons significantly increased in the OGD + miR-7a-2-3p group (∗∗P < 0.01).CC BY · PubMed Central
FIGURE 6 Target Gene Prediction of miRNA-7a-2-3p. (A) The intersections of the predicted target genes results of miR-7a-2-3p in TargetScan, miRDB, miRwalk and miRNA sequencing are presented. The different colors represent distinctive methods of prediction. There were five target genes of miR-7a-2-3p, including VIM, PLAG1, DUSP10, NQO1, and TNFRSF1B. (B) The qRT-PCR analysis showed that VIM, PLAG1, DUSP10, NQO1, and TNFRSF1B. VIM, PLAG1, DUSP10, NQO1, and TNFRSF1B were significantly increased in the HI group compared with the sham group (∗∗P < 0.01). (C) Alignment of the seed regions of miR-7a-2-3p with 3’-UTR of VIM, PLAG1, DUSP10, NQO1, and TNFRSF1B is shown. The red color indicated the pairing bases.
FIGURE 6 Target Gene Prediction of miRNA-7a-2-3p. (A) The intersections of the predicted target genes results of miR-7a-2-3p in TargetScan, miRDB, miRwalk and miRNA sequencing are presented. The different colors represent distinctive methods of prediction. There were five target genes of miR-7a-2-3p, including VIM, PLAG1, DUSP10, NQO1, and TNFRSF1B. (B) The qRT-PCR analysis showed that VIM, PLAG1, DUSP10, NQO1, and TNFRSF1B. VIM, PLAG1, DUSP10, NQO1, and TNFRSF1B were significantly increased in the HI group compared with the sham group (∗∗P < 0.01). (C) Alignment of the seed regions of miR-7a-2-3p with 3’-UTR of VIM, PLAG1, DUSP10, NQO1, and TNFRSF1B is shown. The red color indicated the pairing bases.CC BY · PubMed Central

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Metadata

Authors
Zhang, Z.-B., Tan, Y.-X., Zhao, Q., Xiong, L.-L., Liu, J., Xu, F.-F., Xu, Y., Bobrovskaya, L., Zhou, X.-F., & Wang, T.-H.
Venue
Frontiers in Neuroscience
Publisher
Frontiers Media SA
Published
2019-01-23
Volume
13
ISSN
1662-453X
DOI
10.3389/fnins.2019.00016
PMC
PMC6351497
Cited by
30
References
1
Licence
creativecommons.org/licenses/by/4.0/ (vor)

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