Characterization of blood-derived exosomal hTERT mRNA as a biomarker for colon cancer and Lynch syndrome
Open accessAbstract
Background: Human telomerase reverse transcriptase (hTERT)- mRNA was shown to be elevated in exosomes derived from the sera of a variety of hematological and solid cancer patients. We aimed to evaluate its role as a diagnostic marker in patients with newly diagnosed colon cancer and in hereditary syndromes with predisposition to colon cancer. Methods: hTERT -mRNA levels were determined in serum-derived exosomes from 88 patients with colon cancer, 71 Lynch-syndrome carriers with unknown active malignancies and 50 healthy controls. Data, including demographics, background diseases, clinical data regarding tumor characteristics and genetic data, were retrieved data from medical files. Results: Patients with colon cancer had both higher exosomal hTERT mRNA levels and a higher proportion of patients with positive exosomal hTERT mRNA than controls (29.5% vs. 4%, respectively, P values < 0.001). Within the cancer group, patients with a metastatic disease had higher levels of telomerase mRNA than non-metastatic disease patients, and these levels correlated with CEA levels. Likewise, Lynch syndrome carriers had a higher proportion of positive exosomal hTERT mRNA than controls (21.1% vs. 4%, respectively, P value 0.008) but only a trend towards higher exosomal hTERT mRNA levels. Higher telomerase mRNA levels were not correlated with the mutated gene. Conclusions: Exosomal serum hTERT –mRNA levels are associated with metastatic colon cancer and were also demonstrated in a subset of Lynch syndrome carriers. Its significance as a biomarker for developing malignancy should be elucidated.
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Exosomes offer the advantage that their mRNA content is not exposed to the activity of extracellular RNases, allowing for reliable estimation.
“Exosomes offer the advantage that their mRNA content is not exposed to the activity of extracellular RNases, allowing for reliable estimation [210].”
From Current and Future Trends of Colorectal Cancer Treatment: Exploring Advances in Immunotherapy (2024) · cites this paper as [210]
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Current and Future Trends of Colorectal Cancer Treatment: Exploring Advances in Immunotherapy
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- Authors
- Laish, I., Levi, Z., Mahajna, H., Albshesh, A., Horesh, N., Katz, E., Feldman, D., Shinar, N., Picard, O., Yavzori, M., Fudim, E., Raanani, P., Berger, T., Goldvaser, H., Beery, E., & Uziel, O.
- Affiliations
- Tel Aviv University (IL)
- Corresponding author
- Laish, Ido
- ORCID
- Venue
- Frontiers in Oncology
- Publisher
- Frontiers Media SA
- Published
- 2022-09-20
- Volume
- 12
- Pages
- 962473-962473
- ISSN
- 2234-943X (ISSN-L)
- PMC
- PMC9530579
- OpenAlex
- W4297905831
- Cited by
- 6
- References
- 32
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 2
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Oncology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Keywords
- hTERT mRNA, exosomes, serum exosomes, colon cancer, Lynch syndrome, tumor markers, metastatic colon cancer, carcinoembryonic antigen, hereditary cancer predisposition, telomerase
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