Naturally occurring TPE-CA maintains gut microbiota and bile acids homeostasis via FXR signaling modulation of the liver-gut axis
Open accessAbstract
Antibiotics-induceds changes in intestinal flora (dysbiosis) may have various effects on the host. Dysbiosis is associated with numerous metabolites including bile acids, which are produced in the liver from cholesterol and metabolized in the gut by intestinal microbiota. Total phenolic extracts of Citrus aurantium L. (TPE-CA) are rich in dietary flavanones and their glycosyl derivatives, including flavones, flavonols, polymethoxyflavones and coumarins, which exert positive health effects on the microbiota. The aim of this study is to elucidate the interplays between the intestinal microbiota and bile acids metabolism attributed to antibiotics. Mice were exposed to broad-spectrum antibiotics, such as ampicillin, streptomycin and clindamycin, for 14 days. This exposure resulted in reduced bacterial diversity and richness, and destroyed intestinal permeability. The homeostasis of bile acids was also affected. Subsequent TPE-CA administration, counteracted most of the dysbiosis, and reshaped intestinal permeability, these effects occurred via upregulation of zonula occludens 1 and occludin associated proteins and downregulation of serum endotoxin compared to the antibiotics group. TPE-CA maintained the homeostasis of bile acids via modulation of the liver -gut axis related farnesoid X receptor (FXR)/ fibroblast growth factor 15 (FGF15) pathway and FXR-targeted protein. Our findings indicated that TPE-CA exerted a protective effect on the restoration of intestinal microbiota composition, reshaped barrier integrity and maintained bile acid homeostasis via the liver - gut axis with antibiotics-induced dysbiosis.
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TPE-CA treatment induced the expression of tight junction proteins such as ZO-1 and OCLN-associated proteins in these mice and improved the barrier integrity.
“TPE-CA treatment induced the expression of TJ proteins such as ZO-1 and OCLN-associated proteins in these mice and improved the barrier integrity.112”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [112]
Mice exposed to broad-spectrum antibiotics such as ampicillin, clindamycin and streptomycin resulted in reduced gut bacterial diversity and richness and impacted gut barrier integrity.
“It was showed that mice exposed to broad-spectrum antibiotics such as ampicillin, clindamycin and streptomycin resulted in reduced gut bacterial diversity and richness and impacted gut barrier integrity.112”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [112]
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Regulation of Intestinal Barrier Function by Microbial Metabolites
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- Authors
- Liu, L., Liu, Z., Li, H., Cao, Z., Li, W., Song, Z., Li, X., Lu, A., Lu, C., & Liu, Y.
- Affiliations
- Beijing University of Chinese Medicine (CN)
- China Academy of Chinese Medical Sciences (CN)
- Corresponding author
- Liu, Zhenli
- Lu, Cheng
- Liu, Yuanyan
- Venue
- Frontiers in Pharmacology
- Publisher
- Frontiers Media SA
- Published
- 2020-02-06
- Volume
- 11
- Pages
- 12-12
- ISSN
- 1663-9812 (ISSN-L)
- PMC
- PMC7015895
- OpenAlex
- W2998935115
- Cited by
- 68
- References
- 48
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 17 · 2023: 12 · 2022: 14 · 2021: 10 · 2020: 5
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Pharmacology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Beijing University of Chinese Medicine
- National Science and Technology Major Project (2018ZX10101001-005-003, 2018ZX10101‐001)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- Citrus aurantium, gut microbiota, bile acid homeostasis, FXR signaling, FGF15, gut-liver axis, antibiotic-induced dysbiosis, intestinal permeability, tight junction proteins, endotoxin, flavanones, coumarins, zonula occludens-1, occludin
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