Cited by 204Phytochemicals and Antioxidant ActivitiesAntioxidant Activity and Oxidative StressNatural Antidiabetic Agents Studies

Purple Corn ( Zea mays L.) Phenolic Compounds Profile and Its Assessment as an Agent Against Oxidative Stress in Isolated Mouse Organs

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Ramos-Escudero, Fernando, Muñoz, Ana María, Alvarado-Ortíz, Carlos, Alvarado, Ángel, Yáñez, Jaime A.·2012·Journal of Medicinal Food

Abstract

This study was designed to determine the contents of total polyphenols, flavonoids, flavonols, flavanols, and anthocyanins of purple corn (Zea mays L.) extracts obtained with different methanol:water concentrations, acidified with 1% HCl (1 N). Another objective was to determine the antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and deoxyribose assay, individual phenolic compounds by high-performance liquid chromatography (HPLC), and endogenous antioxidant enzyme (superoxide dismutase [SOD], catalase [CAT], and total peroxidase [TPX]) activity and lipid peroxidation activity (thiobarbituric acid-reactive substances [TBARS] assay) in isolated mouse organs. Overall, the highest total content of polyphenols, anthocyanins, flavonoids, flavonols, and flavanols was obtained with the 80:20 methanol:water extract, acidified with 1% HCl (1 N). The 50% inhibitory concentration values obtained by the DPPH and ABTS assays with this extract were 66.3 μg/mL and 250 μg/mL, respectively. The antioxidant activity by the FRAP assay was 26.1 μM Trolox equivalents/g, whereas the deoxyribose assay presented 93.6% inhibition. Because of these results, the 80:20 methanol:water extract, acidified with 1% HCl (1 N), was used for the remaining tests. Eight phenolic compounds were identified by HPLC: chlorogenic acid, caffeic acid, rutin, ferulic acid, morin, quercetin, naringenin, and kaempferol. Furthermore, it was observed that the purple corn extract was capable of significantly reducing lipid peroxidation (lower malondialdehyde [MDA] concentrations by the TBARS assay) and at the same time increasing endogenous antioxidant enzyme (CAT, TPX, and SOD) activities in isolated mouse kidney, liver, and brain. On the basis of the results, it was concluded that the purple corn extract contained various bioactive phenolic compounds that exhibited considerable in vitro antioxidant activity, which correlated well with the decreased MDA formation and increase in activity of endogenous antioxidant enzymes observed in the isolated mouse organs. This warrants further in vivo studies with purple corn extracts to assess its antioxidant activity and other bioactivities.

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Crossref · 10.1089/jmf.2010.0342

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Authors
Ramos-Escudero, F., Muñoz, A. M., Alvarado-Ortíz, C., Alvarado, Á., & Yáñez, J. A.
Affiliations
  • Universidad de San Martín de Porres (PE)
  • Alcon (United States) (US)
Corresponding author
Yáñez, Jaime A.
Venue
Journal of Medicinal Food
Published
2012-02
Volume
15
Issue
2
Pages
206-215
ISSN
  • 1096-620X (ISSN-L)
  • 1557-7600
OpenAlex
W1966227347
Cited by
204
References
59
Citations by year
2026: 7 · 2025: 19 · 2024: 41 · 2023: 22 · 2022: 19 · 2021: 11 · 2020: 16 · 2019: 8 · 2018: 17 · 2017: 13 · 2016: 8 · 2015: 9 · 2014: 7 · 2013: 3 · 2012: 3
FWCI
2.92
Type
journal article
Language
en
Open access
Locations
  • Journal of Medicinal Food (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-nd)
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
Keywords
purple corn, maize, phenolic compounds, anthocyanins, flavonoids, antioxidant activity, lipid peroxidation, oxidative stress, superoxide dismutase, catalase, peroxidase, chlorogenic acid, caffeic acid, ferulic acid, quercetin, kaempferol, naringenin, morin, rutin, malondialdehyde, kidney, liver, brain, methanol-water extraction, acidification
MeSH terms
Animals, Animal Structures, Antioxidants, Zea mays, Male, Phenols, Plant Extracts, Oxidative Stress, Mice

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