Purple Corn ( Zea mays L.) Phenolic Compounds Profile and Its Assessment as an Agent Against Oxidative Stress in Isolated Mouse Organs
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This study was designed to determine the contents of total polyphenols, flavonoids, flavonols, flavanols, and anthocyanins of purple corn (Zea mays L.) extracts obtained with different methanol:water concentrations, acidified with 1% HCl (1 N). Another objective was to determine the antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and deoxyribose assay, individual phenolic compounds by high-performance liquid chromatography (HPLC), and endogenous antioxidant enzyme (superoxide dismutase [SOD], catalase [CAT], and total peroxidase [TPX]) activity and lipid peroxidation activity (thiobarbituric acid-reactive substances [TBARS] assay) in isolated mouse organs. Overall, the highest total content of polyphenols, anthocyanins, flavonoids, flavonols, and flavanols was obtained with the 80:20 methanol:water extract, acidified with 1% HCl (1 N). The 50% inhibitory concentration values obtained by the DPPH and ABTS assays with this extract were 66.3 μg/mL and 250 μg/mL, respectively. The antioxidant activity by the FRAP assay was 26.1 μM Trolox equivalents/g, whereas the deoxyribose assay presented 93.6% inhibition. Because of these results, the 80:20 methanol:water extract, acidified with 1% HCl (1 N), was used for the remaining tests. Eight phenolic compounds were identified by HPLC: chlorogenic acid, caffeic acid, rutin, ferulic acid, morin, quercetin, naringenin, and kaempferol. Furthermore, it was observed that the purple corn extract was capable of significantly reducing lipid peroxidation (lower malondialdehyde [MDA] concentrations by the TBARS assay) and at the same time increasing endogenous antioxidant enzyme (CAT, TPX, and SOD) activities in isolated mouse kidney, liver, and brain. On the basis of the results, it was concluded that the purple corn extract contained various bioactive phenolic compounds that exhibited considerable in vitro antioxidant activity, which correlated well with the decreased MDA formation and increase in activity of endogenous antioxidant enzymes observed in the isolated mouse organs. This warrants further in vivo studies with purple corn extracts to assess its antioxidant activity and other bioactivities.
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Total polyphenols were quantified by the Folin-Ciocalteu method.
“Total polyphenols were quantified by the Folin-Ciocalteu method [21].”
From Functional and Bioactive Characterization of Hemp Cake Proteins and Polyphenols from Non-Psychoactive Cannabis sativa (2025) · cites this paper as [21]
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Self-citation
- Authors
- Ramos-Escudero, F., Muñoz, A. M., Alvarado-Ortíz, C., Alvarado, Á., & Yáñez, J. A.
- Affiliations
- Universidad de San Martín de Porres (PE)
- Alcon (United States) (US)
- Corresponding author
- Yáñez, Jaime A.
- Venue
- Journal of Medicinal Food
- Publisher
- SAGE Publications
- Published
- 2012-02
- Volume
- 15
- Issue
- 2
- Pages
- 206-215
- ISSN
- 1096-620X (ISSN-L)
- 1557-7600
- PMC
- PMC3264953
- OpenAlex
- W1966227347
- Cited by
- 204
- References
- 59
- Citations by year
- 2026: 7 · 2025: 19 · 2024: 41 · 2023: 22 · 2022: 19 · 2021: 11 · 2020: 16 · 2019: 8 · 2018: 17 · 2017: 13 · 2016: 8 · 2015: 9 · 2014: 7 · 2013: 3 · 2012: 3
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Medicinal Food (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-nd)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
- Keywords
- purple corn, maize, phenolic compounds, anthocyanins, flavonoids, antioxidant activity, lipid peroxidation, oxidative stress, superoxide dismutase, catalase, peroxidase, chlorogenic acid, caffeic acid, ferulic acid, quercetin, kaempferol, naringenin, morin, rutin, malondialdehyde, kidney, liver, brain, methanol-water extraction, acidification
- MeSH terms
- Animals, Animal Structures, Antioxidants, Zea mays, Male, Phenols, Plant Extracts, Oxidative Stress, Mice
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