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Cited by 6Muscle Physiology and DisordersMicroRNA in disease regulationGenetic and phenotypic traits in livestock

Identification of miRNA–mRNA Networks Associated with Pigeon Skeletal Muscle Development and Growth

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Ding, Hao, Chen, Can, Zhang, Tao, Chen, Lan, Chen, Weilin, Ling, Xuanze, Zhang, Genxi, Wang, Jinyu +2 more·2022·Animals
DOI 10.3390/ani12192509PMC PMC9558527
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Abstract

The growth and development of skeletal muscle determine the productivity of pigeon meat production, and miRNA plays an important role in the growth and development of this type of muscle. However, there are few reports regarding miRNA regulating the growth and development of skeletal muscle in pigeons. To explore the function of miRNA in regulating the growth and development of pigeon skeletal muscle, we used RNA sequencing technology to study the transcriptome of pigeons at two embryonic stages (E8 and E13) and two growth stages (D1 and D10). A total of 32,527 mRNAs were identified in pigeon skeletal muscles, including 14,378 novel mRNAs and 18,149 known mRNAs. A total of 2362 miRNAs were identified, including 1758 known miRNAs and 624 novel miRNAs. In total, 839 differentially expressed miRNAs (DEmiRNAs) and 11,311 differentially expressed mRNAs (DEGs) were identified. STEM clustering analysis assigned DEmiRNAs to 20 profiles, of which 7 were significantly enriched (p-value < 0.05). These seven significantly enriched profiles can be classified into two categories. The first category represents DEmiRNAs continuously downregulated from the developmental stage to the growth stage of pigeon skeletal muscle, and the second category represents DEmiRNAs with low expression at the development and early growth stage, and significant upregulation at the high growth stage. We then constructed an miRNA–mRNA network based on target relationships between DEmiRNAs and DEGs belonging to the seven significantly enriched profiles. Based on the connectivity degree, 20 hub miRNAs responsible for pigeon skeletal muscle development and growth were identified, including cli-miR-20b-5p, miR-130-y, cli-miR-106-5p, cli-miR-181b-5p, miR-1-z, cli-miR-1a-3p, miR-23-y, cli-miR-30d-5p, miR-1-y, etc. The hub miRNAs involved in the miRNA–mRNA regulatory networks and their expression patterns during the development and growth of pigeon skeletal muscle were visualized. GO and KEGG enrichment analysis found potential biological processes and pathways related to muscle growth and development. Our findings expand the knowledge of miRNA expression in pigeons and provide a database for further investigation of the miRNA–mRNA regulatory mechanism underlying pigeon skeletal muscle development and growth.

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  • In pigeons and mice, miR-23 has also been found to be a hub for skeletal muscle development.

    “In other species, such as pigeons and mice, miR-23 has also been found to be a hub for skeletal muscle development.”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [107]

    Results §3.6 identifies miR-23-y as a hub miRNA in a network associated with pigeon skeletal muscle growth and development. The paper does not identify miR-23 as a hub in mice; its Introduction mentions mice only in connection with miR-223-3p. The pigeon finding is also a network-based identification, not a demonstrated muscle-development function. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • The abundance of miR-23a functional clusters expressed in skeletal muscle is not stable enough, and mice lacking this functional cluster quickly downregulate the expression level of mature miR-23a.

    “the abundance of miR-23a functional clusters expressed in skeletal muscle is not stable enough, and mice lacking this functional cluster quickly downregulate the expression level of mature miR-23a [107,108].”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [107]

    The Results (§3.6) identify miR-23-y as a hub miRNA in pigeon skeletal muscle, but do not address miR-23a functional-cluster stability or mice lacking that cluster. Searches for miR-23a and cluster knockout effects found no passage reporting a decline in mature miR-23a. (LLM, conf=0.76)

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Authors
Ding, H., Chen, C., Zhang, T., Chen, L., Chen, W., Ling, X., Zhang, G., Wang, J., Xie, K., & Dai, G.
Affiliations
Yangzhou University (CN)
Corresponding author
Zhang, Tao
ORCID
Venue
Animals
Publisher
MDPI AG
Published
2022-09-21
Volume
12
Issue
19
Pages
2509
ISSN
2076-2615 (ISSN-L)
DOI
10.3390/ani12192509
PMC
PMC9558527
OpenAlex
W4296744935
Cited by
6
References
35
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 1
FWCI
0.41
Type
journal article
Language
en
Open access
Licence
Locations
  • Animals (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (NSFC) (32102532, JILAR-KF202016, 2019CXJ168)
  • the Priority Academic Program Development of Jiangsu Higher Education Institutions (32102532, JILAR-KF202016, 2019CXJ168)
  • the Open Project Program of Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University (32102532, JILAR-KF202016, 2019CXJ168)
  • Yangzhou University Science and Technique Innovation Foundation (32102532, JILAR-KF202016, 2019CXJ168)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
Keywords
miRNA-mRNA regulatory network, myogenesis, muscle growth, differentially expressed miRNAs, hub miRNAs, novel miRNAs, embryonic development, meat production, miR-1, miR-20b-5p

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