Cited by 79Phytochemicals and Antioxidant ActivitiesNatural Antidiabetic Agents StudiesPhytochemistry and Biological Activities

HPLC-ESI-qTOF-MS/MS Characterization, Antioxidant Activities and Inhibitory Ability of Digestive Enzymes with Molecular Docking Analysis of Various Parts of Raspberry (Rubus ideaus L.)

Open access
Wu, Lingfeng, Liu, Yufeng, Qin, Yin, Wang, Lu, Wu, Zhenqiang·2019·Antioxidants

Abstract

The anti-oxidative phenolic compounds in plant extracts possess multiple pharmacological functions. However, the phenolic characterization and in vitro bio-activities in various parts of raspberry (Rubus idaeus L.) have not been investigated systematically. In the present study, the phenolic profiles of leaves (LE), fruit pulp (FPE), and seed extracts (SE) in raspberry were analyzed by HR-HPLC-ESI-qTOF-MS/MS method, and their antioxidant activities and digestive enzymes inhibitory abilities were also investigated. The molecular docking analysis was used to delineate their inhibition mechanisms toward type II diabetes related digestive enzymes. Regardless of LE, FPE, or SE, 50% methanol was the best solvent for extracting high contents of phenolic compounds, followed by 50% ethanol and 100% methanol. The LE of raspberry displayed the highest total phenolic content (TPC) and total flavonoid content (TFC). A total of nineteen phenolic compounds were identified. The quantitative results showed that gallic acid, ellagic acid, and procyanidin C3 were the major constituents in the three extracts. The various parts extracts of raspberry all exhibited the strong antioxidant activities, especially for LE. Moreover, the powerful inhibitory effects of the three extracts against digestive enzymes (α-glucosidase and α-amylase) were observed. The major phenolic compounds of the three extracts also showed good inhibitory activities of digestive enzyme in a dose-dependent manner. The underlying inhibitory mechanisms of the main phenolic compounds against digestive enzymes were clarified by molecular docking analysis. The present study demonstrated that the various parts of raspberry had strong antioxidant activities and inhibitory effects on digestive enzymes, and can potentially prevent oxidative damage or diabetes-related problems.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3390/antiox8080274

Cites

40 of 40 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Chemical profiling of Rubus idaeus found the highest total phenolic and flavonoid contents in the leaves, which exhibit antioxidant activities and inhibit digestive enzymes α-glucosidase and α-amylase from producing monosaccharides.

    “Chemical profiling of Rubus idaeus found the highest total phenolic and flavonoid contents in the leaves, which exhibit antioxidant activities and inhibit digestive enzymes, α-glucosidase, and α-amylase from producing monosaccharides [36].”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Wu, L., Liu, Y., Qin, Y., Wang, L., & Wu, Z.
Affiliations
  • Hainan University (CN)
  • South China University of Technology (CN)
  • Guizhou Institute of Technology (CN)
Corresponding author
  • Wang, Lu
  • Wu, Zhenqiang
Venue
Antioxidants
Publisher
MDPI AG
Published
2019-08-03
Volume
8
Issue
8
Pages
274
ISSN
2076-3921 (ISSN-L)
OpenAlex
W2964737477
Cited by
79
References
40
Citations by year
2026: 9 · 2025: 6 · 2024: 9 · 2023: 17 · 2022: 9 · 2021: 11 · 2020: 14 · 2019: 4
FWCI
5.71
Type
journal article
Language
en
Open access
Locations
  • Antioxidants (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • the Scientific Research Foundation of Hainan University (KYQD1901)
  • Guangdong special funds for science and technology innovation strategy, China (JK2018-352-0203)
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
Keywords
raspberry, phenolic compounds, antioxidant activity, α-glucosidase inhibition, α-amylase inhibition, digestive enzyme inhibition, type 2 diabetes, gallic acid, ellagic acid, total phenolic content, total flavonoid content, plant extracts, extraction solvent optimization, leaves, fruit pulp, seeds

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review