Cited by 74Antioxidants, Aging, Portulaca oleraceaPhytochemicals and Antioxidant ActivitiesGenetics, Aging, and Longevity in Model Organisms

Antioxidant Effects of Walnut (Juglans regia L.) Kernel and Walnut Septum Extract in a D-Galactose-Induced Aging Model and in Naturally Aged Rats

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Rusu, Marius Emil, Georgiu, Carmen, Pop, Anca, Mocan, Andrei, Kiss, Bela, Vostinaru, Oliviu, Fizesan, Ionel, Stefan, Maria-Georgia +7 more·2020·Antioxidants

Abstract

Antioxidant dietary intervention is considered a potential strategy in delaying age-related dysfunctions. In this study of 56 days, we assessed the antioxidant effects of walnut kernel (WK) and walnut septum extract (WSE) in a D-galactose (D-gal)-induced aging model and in a naturally aged rat model. Young Wistar rats, treated with D-gal (1200 mg/week), and old rats received daily WK or WSE added to the feed. After 8 weeks, blood, liver, and brain samples were collected and hematological, biochemical, oxidative stress biomarkers, histological, and immunohistochemical analyses were performed. Moreover, acetylcholinesterase activity was investigated in brain homogenates. The outcomes demonstrated significant improvement in cellular antioxidant activity and/or decrease of reactive oxygen species, advanced glycation end products, nitric oxide, malondialdehyde, or increase of glutathione after WK or WSE intake in both models. Additionally, WSE showed hypoglycemic effect, and both WK and WSE lowered acetylcholinesterase activity. Both diets could protect neurons against the induced senescence and could reverse the pathological conditions in the physiological aged brain. Thus, dietary supplementation with WK or WSE can maintain the liver and brain health and reduce the risk of age-related diseases, as well as delaying the onset of aging processes.

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Authors
Rusu, M. E., Georgiu, C., Pop, A., Mocan, A., Kiss, B., Vostinaru, O., Fizesan, I., Stefan, M.-G., Gheldiu, A.-M., Mates, L., Moldovan, R., Muntean, D. M., Loghin, F., Vlase, L., & Popa, D.-S.
Affiliations
  • Iuliu Hațieganu University of Medicine and Pharmacy (RO)
  • Department of Pharmacology, Physiology and Physiopathology, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, Romania
  • Department of Toxicology, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, Romania
Corresponding author
  • Pop, Anca
  • Vlase, Laurian
Venue
Antioxidants
Publisher
MDPI AG
Published
2020-05-14
Volume
9
Issue
5
Pages
424
ISSN
2076-3921 (ISSN-L)
OpenAlex
W3024245268
Cited by
74
References
60
Citations by year
2026: 6 · 2025: 8 · 2024: 13 · 2023: 16 · 2022: 13 · 2021: 16 · 2020: 2
FWCI
5.39
Type
journal article
Language
en
Open access
Locations
  • Antioxidants (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
Keywords
walnut kernel, Juglans regia, D-galactose-induced aging, dietary antioxidant supplementation, oxidative stress, advanced glycation end products, acetylcholinesterase, neuroprotection, hypoglycemic effect, aging rats, age-related diseases, liver health

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