Optimization of the Ultrasound-Assisted Extraction of Bioactive Compounds from Cannabis sativa L. Leaves and Inflorescences Using Response Surface Methodology
Open accessAbstract
This study investigated the effects of particle size and ultrasonic parameters on the yields of bioactive compounds extracted from the leaves and inflorescences of hemp. The total flavonoid and anthocyanin contents were determined using the spectrophotometric method. The response surface methodology (RMS) was employed to optimize the yield of bioactive substances. On the basis of the developed model, the highest flavonoid yield was obtained under the following extraction conditions: particle size, 0.59 mm; extraction time, 10.71 min; ultrasound intensity, 7.13 W∙cm−2; extraction yield, 9.28 mg QE∙g−1; determination coefficient, R2 = 0.97. The optimal conditions for extracting anthocyanins were as follows: particle size, 0.25 mm; extraction time, 15 min; ultrasound intensity, 8.60 W∙cm−2; extraction efficiency, 20.27 mg Cy-GE∙100 g−1; determination coefficient, R2 = 0.87. This study helped confirm the importance of pulsed ultrasound-assisted extraction in obtaining bioactive compounds from hemp.
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Ultrasound-assisted extraction was used to produce extracts from cannabis containing cannabinoids, flavonoids, anthocyanin, stilbenoids, lignans, and oil.
“ultrasound-assisted extraction (UAE) was used to produce extracts from cannabis containing cannabinoids, flavonoids, anthocyanin, stilbenoids, lignans, and oil [43,44,45,46].”
From Supercritical Carbon Dioxide Technology for Recovering Valuable Phytochemicals from Cannabis sativa L. and Valorization of Its Biomass for Food Applications (2023) · cites this paper as [44]
Cites
Kumar, K., Srivastav, S., & Sharanagat, V. S. (2020) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2020.105325 · Cited by 1,330 · see citing works
Mattioli, R., Francioso, A., Mosca, L., & Silva, P. (2020) · Molecules · doi:10.3390/molecules25173809 · Cited by 1,089 · see citing works
Hempseed as a nutritional resource: An overview
Callaway, J. C. (2004) · Euphytica · doi:10.1007/s10681-004-4811-6 · Cited by 910 · see citing works
Farinon, B., Molinari, R., Costantini, L., & Merendino, N. (2020) · Nutrients · doi:10.3390/nu12071935 · Cited by 473 · see citing works
Ghafoor, K., Choi, Y. H., Jeon, J. Y., & Jo, I. H. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf9001439 · Cited by 451 · see citing works
Pellati, F., Borgonetti, V., Brighenti, V., Biagi, M., Benvenuti, S., & Corsi, L. (2018) · BioMed Research International · doi:10.1155/2018/1691428 · Cited by 375 · see citing works
Al-Dhabi, N. A., Karuppiah, P., & Jeganathan, P. M. (2016) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2016.05.005 · Cited by 356 · see citing works
Applications of ultrasound in food and bioprocessing.
Ashokkumar, M. (2015) · Ultrasonics sonochemistry · doi:10.1016/j.ultsonch.2014.08.012 · Cited by 317 · see citing works
Effect of ultrasound processing on anthocyanins and color of red grape juice.
Tiwari, B., Patras, A., Brunton, N. P., Cullen, P. J., & O'Donnell, C. (2010) · Ultrasonics sonochemistry · doi:10.1016/j.ultsonch.2009.10.009 · Cited by 300 · see citing works
Seed composition of ten industrial hemp cultivars approved for production in Canada
Vonapartis, E., Aubin, M., Séguin, P., Mustafa, A. F., & Charron, J. (2014) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2014.11.004 · Cited by 294 · see citing works
Rupasinghe, H. P. V., Davis, A., Kumar, S. K., Murray, B. J., & Zheljazkov, V. D. (2020) · Molecules · doi:10.3390/molecules25184078 · Cited by 272 · see citing works
González-Centeno, M. R., Knoerzer, K., Sabarez, H., Simal, S., Rosselló, C., & Femenia, A. (2014) · Ultrasonics sonochemistry · doi:10.1016/j.ultsonch.2014.01.021 · Cited by 236 · see citing works
Nutraceutical potential of hemp (Cannabis sativa L.) seeds and sprouts
Frassinetti, S., Moccia, E., Caltavuturo, L., Gabriele, M., Longo, V. G., Bellani, L., Giorgi, G., & Giorgetti, L. (2018) · Food Chemistry · doi:10.1016/j.foodchem.2018.04.078 · Cited by 232 · see citing works
Anti-Cancer Potential of Cannabinoids, Terpenes, and Flavonoids Present in Cannabis
Tomko, A. M., Whynot, E. G., Ellis, L. D., & Dupré, D. J. (2020) · Cancers · doi:10.3390/cancers12071985 · Cited by 231 · see citing works
Irakli, M., Tsaliki, E., Kalivas, A., Kleisiaris, F., Sarrou, E., & Cook, C. M. (2019) · Antioxidants · doi:10.3390/antiox8100491 · Cited by 212 · see citing works
Ramić, M., Vidović, S. S., Zeković, Z. P., Vladić, J., Cvejin, A., & Pavlić, B. (2014) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2014.10.002 · Cited by 209 · see citing works
Ultrasound extraction of phenolics and anthocyanins from jabuticaba peel.
Rodrigues, S., Fernandes, F., de Brito, E. D., Sousa, A. D., & Narain, N. (2015) · Industrial Crops and Products · doi:10.1016/j.indcrop.2015.02.059 · Cited by 189 · see citing works
Choi, Y., Kim, H., Hazekamp, A., Erkelens, C., Lefeber, A., & Verpoorte, R. (2004) · Journal of Natural Products · doi:10.1021/np049919c · Cited by 186 · see citing works
Pan, G., Yu, G., Zhu, C., & Qiao, J. (2011) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2011.11.006 · Cited by 179 · see citing works
Izzo, L., Castaldo, L., Narváez, A., Graziani, G., Gaspari, A., Rodríguez-Carrasco, Y., & Ritieni, A. (2020) · Molecules · doi:10.3390/molecules25030631 · Cited by 150 · see citing works
Zou, T., Wang, M., Gan, R.-Y., & Ling, W.-H. (2011) · International Journal of Molecular Sciences · doi:10.3390/ijms12053006 · Cited by 142 · see citing works
Wang, W.-J., Wu, X.-Z., Chantapakul, T., Wang, D., Zhang, S., Ma, X.-B., Ding, T., Ye, X.-Q., & Liu, D.-H. (2017) · Food Research International · doi:10.1016/j.foodres.2017.09.087 · Cited by 116 · see citing works
PKS Activities and Biosynthesis of Cannabinoids and Flavonoids in Cannabis sativa L. Plants
Flores‐Sanchez, I. J., & Verpoorte, R. (2008) · Plant and Cell Physiology · doi:10.1093/pcp/pcn150 · Cited by 94 · see citing works
Effect of ultrasound on the extraction of total anthocyanins from Purple Majesty potato.
Mane, S., Bremner, D., Tziboula-Clarke, A., & Lemos, M. (2015) · Ultrasonics sonochemistry · doi:10.1016/j.ultsonch.2015.06.021 · Cited by 88 · see citing works
Influence of Altitude on Phytochemical Composition of Hemp Inflorescence: A Metabolomic Approach
Giupponi, L., Leoni, V., Pavlovic, R., & Giorgi, A. (2020) · Molecules · doi:10.3390/molecules25061381 · Cited by 85 · see citing works
Starek, A., Kobus, Z., Sagan, A., Chudzik, B., Pawłat, J., Kwiatkowski, M., Terebun, P., & Andrejko, D. (2021) · Scientific Reports · doi:10.1038/s41598-021-83073-8 · Cited by 66
Self-citationNagy, D. U., Cianfaglione, K., Maggi, F., Sut, S., & Dall'acqua, S. (2019) · Chemistry and Biodiversity · doi:10.1002/cbdv.201800562 · Cited by 66 · see citing works
Moccia, S., Siano, F., Russo, G. L., Volpe, M. G., Cara, F. L., Pacifico, S., Piccolella, S., & Picariello, G. (2019) · International Journal of Food Sciences and Nutrition · doi:10.1080/09637486.2019.1666804 · Cited by 56 · see citing works
Kobus, Z., Krzywicka, M., Starek-Wójcicka, A., & Sagan, A. (2022) · Scientific Reports · doi:10.1038/s41598-022-12244-y · Cited by 55 · see citing works
Self-citationKobus, Z., Krzywicka, M., Pecyna, A., & Buczaj, A. (2021) · Energies · doi:10.3390/en14227638 · Cited by 53 · see citing works
Self-citationSystematics and Botanical Characteristics of Industrial Hemp <i>Cannabis Sativa</i> L
Strzelczyk, M., Lochynska, M., & Chudy, M. (2022) · Journal of Natural Fibers · doi:10.1080/15440478.2021.1889443 · Cited by 35 · see citing works
Poniatowska, J., Wielgus, K., Szalata, M., Szalata, M., Ożarowski, M., & Panasiewicz, K. (2019) · Herba Polonica · doi:10.2478/hepo-2019-0012 · Cited by 14 · see citing works
Flagged first, then by citations
1 of the 33 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
de Aguiar, A. C., Vardanega, R., Viganó, J., & Silva, E. K. (2023) · Molecules · doi:10.3390/molecules28093849 · Cited by 51 · see citing works
Driving/Critical Factors Considered During Extraction to Obtain Bioactive Enriched Extracts
Ramesh, M., Shankar, N. S., & Venkatappa, A. (2024) · Pharmacognosy Reviews · doi:10.5530/phrev.2024.18.7 · Cited by 48
“[42] Although additional particle reduction may not considerably increase extraction efficiency after a certain particle size (specify the size), as it may clog the solvent penetration through the fine powder particles.”
BackgroundWani, K. M., & Uppaluri, R. V. S. (2022) · Applied Food Research · doi:10.1016/j.afres.2022.100178 · Cited by 47 · see citing works
Potential Use of Tomato Peel, a Rich Source of Lycopene, for Cancer Treatment
Bolaño, D. C. J., Insuasty, D., Macías, J. D. R., & Grande-Tovar, C. (2024) · Molecules · doi:10.3390/molecules29133079 · Cited by 37 · see citing works
Anthocyanin: Potential tool for diabetes management and different delivery aspects
Sharma, S., Pandita, G., & Bhosale, Y. (2023) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2023.104170 · Cited by 23 · see citing works
Fadil, M., Lebrazi, S., Aboulghazi, A., Baakili, A. E., Es‐Safi, N., Raïs, C., Slimani, C., & Farah, A. (2023) · Sustainable Chemistry and Pharmacy · doi:10.1016/j.scp.2023.101359 · Cited by 25 · see citing works
Jabbari, N., Goli, M., & Shahi, S. (2024) · Foods · doi:10.3390/foods13040542 · Cited by 17 · see citing works
“Each sample was analyzed three times [13–16].”
MethodInfluentialOihabi, M. E., Soultana, M., Imad, E. F., Lanjri, H. F., Allal, L. B., Ammari, M., & Lanjri, A. F. (2024) · Case Studies in Chemical and Environmental Engineering · doi:10.1016/j.cscee.2024.100906 · Cited by 17 · see citing works
Exergoeconomic analysis of ultrasound-assisted extraction of tannins from acorn fruit
Doner, D., & Icier, F. (2023) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2023.111851 · Cited by 16 · see citing works
Nowak, R., Szczepanek, M., Kobus-Cisowska, J., Stuper-Szablewska, K., Dziedziński, M., & Błaszczyk, K. (2023) · PLoS ONE · doi:10.1371/journal.pone.0288428 · Cited by 16 · see citing works
Li, N., Wu, X., Yin, Q., Dong, Z., Zheng, L.-L., Qian, Y.-H., Sun, Y.-L., Chen, Z., & Zhai, K. (2024) · Foods · doi:10.3390/foods13172652 · Cited by 14
“…mL/g), sonication time (20, 40, 60, 80, and 100 min), ethanol concentration (25, 30, 35, 40, and 45%), composite enzyme dosage (0.5, 1.0, 1.5, 2.0, and 2.5%), and enzymatic digestion time (10, 25, 40, 55, and 70 min) on the yield of flavonoids extracted from H. spectabile were examined [17–19].”
MethodInfluentialCharles, A. P. R., Chen, B., & Rao, J. (2024) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2024.2436130 · Cited by 10 · see citing works
“Based on the knowledge gained from the literature, it is evident that the performance of the UAE depends mainly on the extraction time and ultrasound intensity (Kobus et al. 2022).”
Kaminski, K. P., Hoeng, J., Lach-Falcone, K., Goffman, F. D., Schlage, W. K., & Latino, D. A. R. S. (2025) · Molecules · doi:10.3390/molecules30132784 · Cited by 13 · see citing works
“The method demonstrated high efficiency in isolating terpenes and other volatiles, offering a fast, low-temperature alternative to conventional techniques [113,114].”
Kobus, Z., Buczaj, A., Pecyna, A., Kapica, J., Findura, P., & Kocira, S. (2023) · Applied Sciences · doi:10.3390/app13020760 · Cited by 12 · see citing works
Self-citationAtia, D., Zobeidi, A., Nacer, S. N., Ghernaout, D., & Elboughdiri, N. A. (2024) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-024-06033-y · Cited by 10 · see citing works
Prabsangob, N., Hangsalad, S., & Harnsilawat, T. (2024) · Molecules · doi:10.3390/molecules29112511 · Cited by 8 · see citing works
Góral-Kowalczyk, M., Grza̧dka, E., Orzeł, J., Góral, D., Skrzypek, T., Kobus, Z., & Nawrocka, A. (2024) · Materials · doi:10.3390/ma17112515 · Cited by 9
“The total flavonoid content was determined according to the methodology described by Aryal et al. [25] with a slight modification by Kobus et al. [24].”
MethodSelf-citationJaroenkunpanit, P., Wungsintaweekul, J., & Chetpattananondh, P. (2025) · ACS Omega · doi:10.1021/acsomega.5c06069 · Cited by 8 · see citing works
Thanarukwuttikorn, K., Prabsangob, N., Maisuthisakul, P., & Harnsilawat, T. (2025) · Measurement Food · doi:10.1016/j.meafoo.2025.100237 · Cited by 7 · see citing works
Patra, A., Prasath, V. A., Thakur, R., Sree, V. S., Sarkar, P., & Vivek, K. (2024) · Journal of Food Process Engineering · doi:10.1111/jfpe.14723 · Cited by 10 · see citing works
Pecyna, A., Buczaj, A., Różyło, R., & Kobus, Z. (2023) · Applied Sciences · doi:10.3390/app13084889 · Cited by 11 · see citing works
Self-citationEbrahimi, P., Roodbali, A., Simonato, B., Lante, A., & Rizzi, C. (2025) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2025.107375 · Cited by 6 · see citing works
“Similar results have been reported in the previous studies [16,48].”
Baldino, L., Scognamiglio, M., & Reverchon, E. (2025) · The Canadian Journal of Chemical Engineering · doi:10.1002/cjce.25612 · Cited by 5 · see citing works
Krzywicka, M., & Kobus, Z. (2023) · Applied Sciences · doi:10.3390/app13137805 · Cited by 5 · see citing works
Self-citationKobus, Z., & Krzywicka, M. (2023) · Energies · doi:10.3390/en16134966 · Cited by 4 · see citing works
Self-citationLi, J., Tang, L., & Wang, J. (2025) · Processes · doi:10.3390/pr13061905 · Cited by 3 · see citing works
Sunanta, P., Sombat, T., Moaphadungkul, J., Sirin, C., Nagle, M., Bakshani, C. R., Willats, W. G. T., Sangta, J., & Sommano, S. R. (2024) · Journal of Applied Research on Medicinal and Aromatic Plants · doi:10.1016/j.jarmap.2024.100593 · Cited by 3 · see citing works
Rodríguez, J. C. D., Benítez, R. B., & Romero, P. A. A. (2025) · Journal of Molecular Liquids · doi:10.1016/j.molliq.2025.129109 · Cited by 1 · see citing works
Development and Validation of a Cannabinoid Quantification Method in Oil and Marijuana by UHPLC-MS
de Almeida, J. D., Conceição, N. S., Pereira, A. R., Lyrio, M. V. V., Ortiz, R. S., dos Santos, N. D., & Romão, W. (2025) · ACS Omega · doi:10.1021/acsomega.5c07689 · Cited by 1
Matteucci, E., Cattaneo, F., Zampini, I. C., Mercado, M. I., & Isla, M. I. (2024) · Lilloa · doi:10.30550/j.lil/1987 · Cited by 1 · see citing works
Choi, H. W. (2026) · Recent Progress in Nutrition · doi:10.21926/rpn.2603012
Orellana, B. S., Danert, F. C., Zampini, I. C., & Isla, M. I. (2026) · Molecules · doi:10.3390/molecules31111845
“Other authors reported that in the cannabis leaf extracts obtained with 50% ethanol, the highest flavonoid content was 11.21 mg QE · g − 1 DW for young plants and 5.21 mg QE · g − 1 DW for mature plants [32].”
InfluentialSingh, P., Kaur, S., Aggarwal, P., Kaur, N., & Kumar, S. (2026) · Waste and Biomass Valorization · doi:10.1007/s12649-026-03558-3
Miraglio, S. I. R., Raspo, M. A., Costamagna, N., & Andreatta, A. E. (2026) · Drug Development and Industrial Pharmacy · doi:10.1080/03639045.2025.2612300
“Kobus et al. [14] optimized extraction using a Box–Behnken design with three factors—particle size, extraction time, and ultrasound intensity—employing 60% aqueous ethanol. agarwal et al. [15] targeted cannabinoids using methanol, optimizing ultrasound power (90–150 W), extraction time (5–15 min),…”
Li, N., Melencion, M. G., Zheng, L.-L., Qian, Y.-H., & Weng, Q. (2023) · BIO Web of Conferences · doi:10.1051/bioconf/20237201008
“The quadratic terms A2, B2, C2 and D2 had significant effects on the extraction rate of total flavonoids [8] .”
Background
- Authors
- Kobus, Z., Pecyna, A., Buczaj, A., Krzywicka, M., Przywara, A., & Nadulski, R.
- Affiliations
- University of Life Sciences in Lublin (PL)
- Department of Food Engineering and Machines, University of Life Sciences, Głęboka 28, 20-612 Lublin, Poland
- Corresponding author
- Pecyna, Anna
- Venue
- Applied Sciences
- Publisher
- MDPI AG
- Published
- 2022-07-03
- Volume
- 12
- Issue
- 13
- Pages
- 6747
- ISSN
- 2076-3417 (ISSN-L)
- OpenAlex
- W4283795706
- Cited by
- 39
- References
- 33
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 12 · 2023: 10 · 2022: 2
- FWCI
- 4.52
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Applied Sciences (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Pharmacology › Cannabis and Cannabinoid Research
- Keywords
- ultrasound-assisted extraction, Cannabis sativa, hemp leaves, flavonoids, anthocyanins, bioactive compounds, particle size, ultrasound intensity, extraction time, extraction optimization
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