Combining electrostimulation with impedance sensing to promote and track osteogenesis within a titanium implant
Open accessAbstract
(1) Background: Electrical stimulation is a promising alternative to promote bone fracture healing but with the limitation of tracking the osteogenesis progress in vivo. To overcome this issue, we present an opportunity to combine the electrical stimulation of a commercial titanium implant, which promotes osteogenesis within the fracture, with a real-time readout of the osteogenic progress by impedance sensing. This makes it possible to adjust the electrical stimulation modalities to the individual patient's fracture healing process. (2) Methods: In detail, osteogenic differentiation of several cell types was monitored under continuous or pulsatile electrical stimulation at 0.7 V AC/20 Hz for at least seven days on a titanium implant by electric cell-substrate impedance sensing (ECIS). For control, chemical induction of osteogenic differentiation was induced. (3) Results: The most significant challenge was to discriminate impedance changes caused by proliferation events from those initiated by osteogenic differentiation. This discrimination was achieved by remodeling the impedance parameter Alpha (α), which increases over time for pulsatile electrically stimulated stem cells. Boosted α-values were accompanied by an increased formation of actin stress fibers and a reduced expression of the focal adhesion kinase in the cell periphery; morphological alterations known to occur during osteogenesis. (4) Conclusions: This work provided the basis for developing an effective fracture therapy device, which can induce osteogenesis on the one hand, and would allow us to monitor the induction process on the other hand.
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Methods to accelerate fracture healing – a narrative review from a clinical perspective
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- Authors
- Engel, N., Dau, M., Engel, V., Franz, D., Klemmstein, F., Thanisch, C., Kolb, J. F., Frank, M., Springer, A., Köhling, R., Bader, R., Frerich, B., Wiesmann, N., Heimes, D., & Kämmerer, P. W.
- Affiliations
- Universitätsmedizin Rostock (DE)
- University of Rostock (DE)
- ibidi GmbH, Lochhamer Schlag 11, 82166 Gräfelfing, Germany
- Institute of Physics, University of Rostock, Albert-Einstein-Str. 23-24, 18059 Rostock, Germany
- University Medical Center of the Johannes Gutenberg University Mainz (DE)
- Corresponding author
- Kämmerer, Peer W.
- ORCID
- Venue
- Biomedicines
- Publisher
- MDPI AG
- Published
- 2023-02-24
- Volume
- 11
- Issue
- 3
- Pages
- 697
- ISSN
- 2227-9059 (ISSN-L)
- PMC
- PMC10045247
- OpenAlex
- W4322500327
- Cited by
- 10
- References
- 38
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 2 · 2023: 2
- FWCI
- 0.79
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Biomedicines (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) (SFB 1270/1,2-299150580)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Epidemiology › Bone fractures and treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Keywords
- electrical stimulation, impedance sensing, bone formation, titanium implants, fracture healing, osteogenic differentiation, electric cell-substrate impedance sensing, pulsatile stimulation, cell proliferation, stress fibers, focal adhesion kinase, stem cells, real-time monitoring
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