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Selected Technical Aspects of Molecular Allergy Diagnostics

Lis, Kinga, Bartuzi, Zbigniew·2023·Current Issues in Molecular Biology
DOI 10.3390/cimb45070347PMC PMC10378047
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Abstract

Diagnosis of allergic diseases is a complex, multi-stage process. It often requires the use of various diagnostic tools. The in vitro diagnostics (IVD), which includes various laboratory tests, is one of the stages of this process. Standard laboratory tests include the measurement of the serum concentration of specific immunoglobulin E (sIgE) for selected allergens, full allergen extracts and/or single allergen components (molecules). The measurement of IgE sIgE to the allergen components is called molecular allergy diagnosis. During the standard laboratory diagnostic process, various models of immunochemical tests are used, which enable the measurement of sIgE for single allergens (one-parameter tests, singleplex) or IgE specific for many different allergens (multi-parameter tests, multiplex) in one test. Currently, there are many different test kits available, validated for IVD, which differ in the method type and allergen profile. The aim of the manuscript is to present various technical aspects related to modern allergy diagnostics, especially in the area of molecular allergy diagnostics.

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Figure 1 Mechanisms of hypersensitivity reactions.
Figure 1 Mechanisms of hypersensitivity reactions.CC BY · PubMed Central
Figure 2 General scheme of the ELISA reaction detecting allergen-specific.
Figure 2 General scheme of the ELISA reaction detecting allergen-specific.CC BY · PubMed Central
Figure 3 Allergen extract versus allergen components; The colored dots are individual allergen molecules.
Figure 3 Allergen extract versus allergen components; The colored dots are individual allergen molecules.CC BY · PubMed Central
Figure 4 The biochip of the ISACE112i test (A) and scan of the biochip field for a single patient, after reaction with serum, detected in a scanner (LuxScan) with MIA software (B).
Figure 4 The biochip of the ISACE112i test (A) and scan of the biochip field for a single patient, after reaction with serum, detected in a scanner (LuxScan) with MIA software (B).CC BY · PubMed Central
Figure 5 The ALEX2 biochip/cassette with positive reaction (A) and cartridge scan image with positive reaction in scanner (ImageXplorer) with MADx Raptor Software (B) author’s own photo.
Figure 5 The ALEX2 biochip/cassette with positive reaction (A) and cartridge scan image with positive reaction in scanner (ImageXplorer) with MADx Raptor Software (B) author’s own photo.CC BY · PubMed Central

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Citations

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Metadata

Authors
Lis, K., & Bartuzi, Z.
Affiliations
Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-168 Bydgoszcz, Poland
ORCID
Venue
Current Issues in Molecular Biology
Publisher
MDPI AG
Published
2023-06-29
Volume
45
Issue
7
Pages
5481-5493
ISSN
1467-3045
DOI
10.3390/cimb45070347
PMC
PMC10378047
Cited by
24
References
1
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)

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