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Selected Technical Aspects of Molecular Allergy Diagnostics

Lis, Kinga, Bartuzi, Zbigniew·2023·Current Issues in Molecular Biology
DOI 10.3390/cimb45070347PMC PMC10378047
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Abstract

Diagnosis of allergic diseases is a complex, multi-stage process. It often requires the use of various diagnostic tools. The in vitro diagnostics (IVD), which includes various laboratory tests, is one of the stages of this process. Standard laboratory tests include the measurement of the serum concentration of specific immunoglobulin E (sIgE) for selected allergens, full allergen extracts and/or single allergen components (molecules). The measurement of IgE sIgE to the allergen components is called molecular allergy diagnosis. During the standard laboratory diagnostic process, various models of immunochemical tests are used, which enable the measurement of sIgE for single allergens (one-parameter tests, singleplex) or IgE specific for many different allergens (multi-parameter tests, multiplex) in one test. Currently, there are many different test kits available, validated for IVD, which differ in the method type and allergen profile. The aim of the manuscript is to present various technical aspects related to modern allergy diagnostics, especially in the area of molecular allergy diagnostics.

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Figure 1 Mechanisms of hypersensitivity reactions.
Figure 1 Mechanisms of hypersensitivity reactions.CC BY · PubMed Central
Figure 2 General scheme of the ELISA reaction detecting allergen-specific.
Figure 2 General scheme of the ELISA reaction detecting allergen-specific.CC BY · PubMed Central
Figure 3 Allergen extract versus allergen components; The colored dots are individual allergen molecules.
Figure 3 Allergen extract versus allergen components; The colored dots are individual allergen molecules.CC BY · PubMed Central
Figure 4 The biochip of the ISACE112i test (A) and scan of the biochip field for a single patient, after reaction with serum, detected in a scanner (LuxScan) with MIA software (B).
Figure 4 The biochip of the ISACE112i test (A) and scan of the biochip field for a single patient, after reaction with serum, detected in a scanner (LuxScan) with MIA software (B).CC BY · PubMed Central
Figure 5 The ALEX2 biochip/cassette with positive reaction (A) and cartridge scan image with positive reaction in scanner (ImageXplorer) with MADx Raptor Software (B) author’s own photo.
Figure 5 The ALEX2 biochip/cassette with positive reaction (A) and cartridge scan image with positive reaction in scanner (ImageXplorer) with MADx Raptor Software (B) author’s own photo.CC BY · PubMed Central

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Citations

Cites

Research works printed but not linked: 42

  1. Tanno, 2016, Dissemination of definitions and concepts of allergic and hypersensitivity conditions, World Allergy Organ J., 9, 24, 10.1186/s40413-016-0115-2 · DOI
  2. Dispenza, 2019, Classification of hypersensitivity reactions, Allergy Asthma Proc., 40, 470, 10.2500/aap.2019.40.4274 · DOI
  3. Muraro, 2014, EAACI Food Allergy and Anaphylaxis Guidelines Group. EAACI food allergy and anaphylaxis guidelines: Diagnosis and management of food allergy, Allergy, 69, 1008, 10.1111/all.12429 · DOI · Paper page
  4. Ansotegui, 2020, IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper, World Allergy Organ J., 13, 100080, 10.1016/j.waojou.2019.100080 · DOI · Paper page
  5. Jakob, 2015, Molecular allergy diagnostics using IgE singleplex determinations: Methodological and practical considerations for use in clinical routine: Part 18 of the Series Molecular Allergology, Allergo J. Int., 24, 185, 10.1007/s40629-015-0087-8 · DOI
  6. Jakob, 2015, Molecular allergy diagnostics using multiplex assays: Methodological and practical considerations for use in research and clinical routine: Part 21 of the Series Molecular Allergology, Allergo J. Int., 24, 320, 10.1007/s40629-015-0087-8 · DOI
  7. Wauthier, L., Cabo, J., Eucher, C., Rosseels, C., Elsen, M., and Favresse, J. (2023). Biotin interference in immunoassays: Water under the bridge?. Clin. Chem. Lab. Med., advance online publication. · DOI
  8. Liu, D., Gebreab, Y.B., Hu, J., Zhou, L., Zhang, N., Tong, H., Chen, B., and Wang, X. (2022). Development and Evaluation of an Anti-Biotin Interference Method in Biotin-Streptavidin Immunoassays. Diagnostics, 12. · DOI
  9. Yunginger, 2000, Quantitative IgE antibody assays in allergic diseases, J. Allergy Clin. Immunol., 105, 1077, 10.1067/mai.2000.107041 · DOI
  10. Hamilton, 2015, Analytical performance characteristics, quality assurance and clinical utility of immunological assays for human immunoglobulin E (IgE) antibodies of defined allergen specificities, J. Allergy Clin. Immunol., 135, AB8, 10.1016/j.jaci.2014.12.961 · DOI
  11. Schmidta, 2002, Expression systems for production of recombinant allergens, Int. Arch. Allergy Immunol., 128, 264, 10.1159/000063865 · DOI
  12. Tscheppe, 2017, Recombinant Allergens in Structural Biology, Diagnosis, and Immunotherapy, Int. Arch. Allergy Immunol., 172, 187, 10.1159/000464104 · DOI
  13. Curin, 2017, Single recombinant and purified major allergens and peptides: How they are made and how they change allergy diagnosis and treatment, Ann. Allergy Asthma. Immunol., 119, 201, 10.1016/j.anai.2016.11.022 · DOI
  14. Jutel, 2012, Recombinant allergens: The present and the future, Hum. Vaccin. Immunother., 8, 1534, 10.4161/hv.22064 · DOI
  15. Kleine-Tebb, J., and Jakob, T. (2017). Molecular Allergy Diagnostics Innovation for a Better Patient Management, Springer International Publishing. · DOI
  16. Siekierzynska, A., Piasecka-Kwiatkowska, D., Litwinczuk, W., Burzynska, M., Myszka, A., Karpinski, P., Zygala, E., Piorecki, N., Springer, E., and Sozanski, T. (2021). Molecular and Immunological Identification of Low Allergenic Fruits among Old and New Apple Varieties. Int. J. Mol. Sci., 22. · DOI
  17. Ferrari, E., Breda, D., Spisni, A., and Burastero, S.E. (2023). Component-Resolved Diagnosis Based on a Recombinant Variant of Mus m 1 Lipocalin Allergen. Int. J. Mol. Sci., 24. · DOI
  18. Luengo, 2022, Molecular Allergy Diagnosis in Clinical Practice: Frequently Asked Questions, J. Investig. Allergol. Clin. Immunol., 32, 1, 10.18176/jiaci.0769 · DOI · Paper page
  19. Barber, 2021, Molecular allergology and its impact in specific allergy diagnosis and therapy, Allergy, 76, 3642, 10.1111/all.14969 · DOI · Paper page
  20. Ansotegui, 2020, A WAO–ARIA–GA2LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020, World Allergy Organ J., 13, 100091, 10.1016/j.waojou.2019.100091 · DOI · Paper page
  21. Neagu, 2019, Protein microarray technology: Assisting personalized medicine in oncology (Review), World Acad. Sci. J., 1, 113
  22. Melioli, 2014, Allergenius, an expert system for the interpretation of allergen microarray results, World Allergy Organ J., 7, 15, 10.1186/1939-4551-7-15 · DOI
  23. Bojcukova, 2019, Comparison of two multiplex arrays in the diagnostics of allergy, Clin. Transl. Allergy, 9, 31, 10.1186/s13601-019-0270-y · DOI
  24. Platteel, 2022, A comprehensive comparison between ISAC and ALEX2 multiplex test systems, Clin. Chem. Lab. Med., 60, 1046, 10.1515/cclm-2022-0191 · DOI
  25. Alessandri, 2017, Diagnosing allergic sensitizations in the third millennium: Why clinicians should know allergen molecule structures, Clin. Transl. Allergy, 7, 21, 10.1186/s13601-017-0158-7 · DOI
  26. Martins, 2014, Biological applications of nanobiotechnology, J. Nanosci. Nanotechnol., 14, 1007, 10.1166/jnn.2014.8748 · DOI
  27. Salata, 2004, Applications of nanoparticles in biology and medicine, J. Nanotechnol., 2, 3
  28. Reagen, S., and Zhao, J.X. (2022). Analysis of Nanomaterials on Biological and Environmental Systems and New Analytical Methods for Improved Detection. Int. J. Mol. Sci., 23. · DOI
  29. Popescu, 2018, Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules, World J. Methodol., 8, 17, 10.5662/wjm.v8.i3.17 · DOI · Paper page
  30. Heffler, 2018, Extended IgE profile based on an allergen macroarray: A novel tool for precision medicine in allergy diagnosis, World Allergy Organ J., 11, 7, 10.1186/s40413-018-0186-3 · DOI
  31. Kochuyt, 2005, Prevalence and clinical relevance of specific immunoglobulin E to pollen caused by sting-induced specific immunoglobulin E to cross-reacting carbohydrate determinants in Hymenoptera venoms, Clin. Exp. Allergy, 35, 441, 10.1111/j.1365-2222.2005.02217.x · DOI
  32. Mari, 2002, IgE to cross-reactive carbohydrate determinants: Analysis of the distribution and appraisal of the in vivo and in vitro reactivity, Int. Arch. Allergy Immunol., 129, 286, 10.1159/000067591 · DOI
  33. Altmann, 2016, Coping with cross-reactive carbohydrate determinants in allergy diagnosis, Allergo J. Int., 25, 98, 10.1007/s40629-016-0115-3 · DOI · Paper page
  34. Chen, 2022, Cross-Reacting Carbohydrate Determinants Inhibitor Can Improve the Diagnostic Accuracy in Pollen and Food Allergy, J. Asthma Allergy, 15, 713, 10.2147/JAA.S363206 · DOI · Paper page
  35. Sinson, E., Ocampo, C., Liao, C., Nguyen, S., Dinh, L., Rodems, K., Whitters, E., and Hamilton, R.G. (2020). Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components. PLoS ONE, 15. · DOI
  36. Luo, 2021, CCD Inhibition Test Can Improve the Accuracy of the Detection of Pollen and Seed Food Allergen-Specific IgE in Southern China, J. Asthma Allergy, 14, 439, 10.2147/JAA.S302920 · DOI
  37. Holzweber, 2013, Inhibition of IgE binding to cross-reactive carbohydrate determinants enhances diagnostic selectivity, Allergy, 68, 1269, 10.1111/all.12229 · DOI
  38. Villalta, 2018, Recommendations for the use of molecular diagnostics in the diagnosis of allergic diseases, Eur. Ann. Allergy Clin. Immunol., 50, 51, 10.23822/EurAnnACI.1764-1489.32 · DOI
  39. Peveri, 2019, Molecular diagnostics improves diagnosis and treatment of respiratory allergy and food allergy with economic optimization and cost saving, Allergol. Immunopathol., 47, 64, 10.1016/j.aller.2018.05.008 · DOI
  40. Diem, 2022, Real-life evaluation of molecular multiplex IgE test methods in the diagnosis of pollen associated food allergy, Allergy, 77, 3028, 10.1111/all.15329 · DOI
  41. Santos, 2022, Bringing the Next Generation of Food Allergy Diagnostics into the Clinic, J. Allergy Clin. Immunol. Pract., 10, 1, 10.1016/j.jaip.2021.09.009 · DOI · Paper page
  42. Suprun, 2019, Novel Bead-Based Epitope Assay is a sensitive and reliable tool for profiling epitope-specific antibody repertoire in food allergy, Sci. Rep., 9, 18425, 10.1038/s41598-019-54868-7 · DOI · Paper page

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Metadata

Authors
Lis, K., & Bartuzi, Z.
Affiliations
Department of Allergology, Clinical Immunology and Internal Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-168 Bydgoszcz, Poland
ORCID
Venue
Current Issues in Molecular Biology
Publisher
MDPI AG
Published
2023-06-29
Volume
45
Issue
7
Pages
5481-5493
ISSN
1467-3045
DOI
10.3390/cimb45070347
PMC
PMC10378047
Cited by
24
References
1
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)

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