Antimicrobial, Multi-Drug and Colistin Resistance in Enterobacteriaceae in Healthy Pigs in the Greater Accra Region of Ghana, 2022: A Cross-Sectional Study
Open accessAbstract
There is little published information on antimicrobial resistance (AMR) in animals in Ghana. We determined the prevalence and factors associated with AMR, multi-drug resistance (MDR-resistance to ≥3 antimicrobial classes) and colistin resistance in Enterobacteriaceae in healthy pigs in Accra, Ghana. Rectal swabs obtained from the pigs on 20 farms from January to March 2022, were examined for Escherichia coli, Enterobacter spp. and Klebsiella pneumoniae. AMR was determined using standard microbiological techniques and the mcr-1 gene detected through molecular analysis. Enterobacteriaceae were isolated from 197 of 200 pigs: these comprised 195 E. coli isolates, 38 Enterobacter spp. and 3 K. pneumoniae, either singly or combined. Over 60% of E. coli were resistant to tetracycline, with 27% and 34% being resistant to amoxicillin/clavulanic acid and ampicillin, respectively; 23% of E. coli and 5% of Enterobacter spp. exhibited MDR phenotypes. Phenotypic colistin resistance was found in 8% of E. coli and Enterobacter spp., with the mcr-1 gene detected in half. Our study findings should be incorporated into on-going AMR, MDR and colistin resistance surveillance programs in Ghana. We further advocate for tailored-specific education for pig farmers on animal antimicrobial use and for strengthened regulatory policy on antimicrobial usage and monitoring in the animal production industry.
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Six animal studies reported MDR prevalence ranging from 14.9% to 100% in E. coli.
“Six “animal studies” reported MDR prevalence ranging from 14.9% to 100% in E. coli [45,46,48,49,51,63].”
From A Systematic Review on Antimicrobial Resistance in Ghana from a One Health Perspective (2024) · cites this paper as [48]
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- Authors
- Ohene Larbi, R., Adeapena, W., Ayim-Akonor, M., Ansa, E. D. O., Tweya, H., Terry, R. F., Labi, A.-K., & Harries, A. D.
- Affiliations
- CSIR—Animal Research Institute, P.O. Box AH 20 Achimota-Accra, Greater Accra Region, Ghana
- Kintampo Health Research Centre (GH)
- Council for Scientific and Industrial Research (GH)
- Malawi International Training and Education Center for Health (Malawi-I-TECH), Lilongwe P.O. Box 30369, Malawi
- World Health Organization (CH)
- WHO Country Office, P.O. Box MB 142, 7 Ameda Street, Roman Ridge-Accra, Greater Accra Region, Ghana
- London School of Hygiene & Tropical Medicine (GB)
- ORCID
- Venue
- International Journal of Environmental Research and Public Health
- Publisher
- MDPI AG
- Published
- 2022-08-22
- Volume
- 19
- Issue
- 16
- Pages
- 10449
- ISSN
- 1660-4601 (ISSN-L)
- PMC
- PMC9408530
- OpenAlex
- W4292687304
- Semantic Scholar
- 44362b94b9c79b4e237e5cd22d0aa958db41b626
- Cited by
- 14
- References
- 39
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 3 · 2023: 2 · 2022: 1
- FWCI
- 0.96
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Environmental Research and Public Health (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- UK Department of Health & Social Care
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antibiotic Use and Resistance
- Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
- Keywords
- antimicrobial resistance, multidrug resistance, colistin resistance, Enterobacteriaceae, pigs, Escherichia coli, Enterobacter, Klebsiella pneumoniae, mcr-1, tetracycline resistance, ampicillin resistance
- MeSH terms
- Animals, Anti-Bacterial Agents, Colistin, Cross-Sectional Studies, Enterobacteriaceae, Escherichia coli, Ghana, Klebsiella pneumoniae, Microbial Sensitivity Tests, Swine, Drug Resistance, Bacterial, Escherichia coli Proteins
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