Alliin Attenuated RANKL-Induced Osteoclastogenesis by Scavenging Reactive Oxygen Species through Inhibiting Nox1
Open accessAbstract
The healthy skeleton requires a perfect coordination of the formation and degradation of bone. Metabolic bone disease like osteoporosis is resulted from the imbalance of bone formation and/or bone resorption. Osteoporosis also reflects lower level of bone matrix, which is contributed by up-regulated osteoclast-mediated bone resorption. It is reported that monocytes/macrophage progenitor cells or either hematopoietic stem cells (HSCs) gave rise to multinucleated osteoclasts. Thus, inhibition of osteoclastic bone resorption generally seems to be a predominant therapy for treating osteoporosis. Recently, more and more natural compounds have been discovered, which have the ability of inhibiting osteoclast differentiation and fusion. Alliin (S-allyl-l-cysteine sulfoxides, SACSO) is the major component of aged garlic extract (AGE), bearing broad-spectrum natural antioxidant properties. However, its effects on bone health have not yet been explored. Hence, we designed the current study to explore its effects and role in receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast fusion and differentiation. It was revealed that alliin had an inhibitory effect in osteoclasteogenesis with a dose-dependent manner via blocking the c-Fos-NFATc1 signaling pathway. In addition, alliin decreased the generation of reactive oxygen species (ROS) and down-regulated the expression of NADPH oxidase 1 (Nox1). The overall results revealed that alliin could be a potential therapeutic agent in the treatment of osteoporosis.
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Alliin prevents ROS production by inhibiting NADPH oxidase 1, and thus inhibiting osteoclast fusion caused by receptor activator of nuclear factor-kappa B ligand (RANKL).
“Moreover, it was reported to prevent ROS production by inhibiting NADPH oxidase 1, and thus, inhibiting the osteoclast fusion caused by receptor activator of nuclear factor-kappa B ligand (RANKL) [67].”
From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [67]
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Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review
El-Saber Batiha, G., Magdy Beshbishy, A., G. Wasef, L., Elewa, Y. H. A., A. Al-Sagan, A., Abd El-Hack, M. E., Taha, A. E., M. Abd-Elhakim, Y., & Prasad Devkota, H. (2020) · Nutrients · doi:10.3390/nu12030872 · Cited by 931 · see citing works
- Authors
- Chen, Y., Sun, J., Dou, C., Li, N., Kang, F., Wang, Y., Cao, Z., Yang, X., & Dong, S.
- Affiliations
- Army Medical University (CN)
- Corresponding author
- Dong, Shiwu
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2016-09-20
- Volume
- 17
- Issue
- 9
- Pages
- 1516
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC5037793
- OpenAlex
- W2522601370
- Cited by
- 61
- References
- 33
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 7 · 2023: 4 · 2022: 7 · 2021: 7 · 2020: 11 · 2019: 7 · 2018: 4 · 2017: 2
- FWCI
- 2.25
- Type
- journal article
- Language
- en
- Update notices
- correction (2016-09-20)
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- China Postdoctoral Science Foundation
- National High-tech Research and Development Program
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- alliin, RANKL, osteoclastogenesis, osteoporosis, reactive oxygen species, NOX1, bone resorption, aged garlic extract, natural antioxidants
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