Probiotic Properties and Neuroprotective Effects of Lactobacillus buchneri KU200793 Isolated from Korean Fermented Foods
Open accessAbstract
The purpose of this study was to evaluate the probiotic characteristics and neuroprotective effects of bacteria isolated from Korean fermented foods. Three bacterial strains (Lactobacillus fermentum KU200060, Lactobacillus delbrueckii KU200171, and Lactobacillus buchneri KU200793) showed potential probiotic properties, such as high tolerance against artificial gastric juice and bile salts, sensitivity to antibiotics, nonproduction of carcinogenic enzymes, and high adhesion to intestinal cells. Heat-killed L. fermentum KU200060 and L. buchneri KU200793 showed higher antioxidant activity than heat-killed L. delbrueckii KU200171. The conditioned medium (CM) was used to evaluate the reaction between HT-29 cells and each heat-killed strain. All CMs protected SH-SY5Y cells from 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity. The expression of brain-derived neurotropic factor (BDNF) mRNA in HT-29 cells treated with CM containing heat-killed L. buchneri KU200793 was the highest. The CM significantly reduced the Bax/Bcl-2 ratio and increased BDNF mRNA expression in SH-SY5Y cells treated with MPP+. These results indicate that L. buchneri KU200793 can be used as a prophylactic functional food, having probiotic potential and neuroprotective effects.
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The adherence values found were similar to those of other studies for LAB isolated from fermented foods.
“The adherence values found were similar to those of other studies for LAB isolated from fermented foods [41,46].”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [46]
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- Authors
- Cheon, M.-J., Lim, S.-M., Lee, N.-K., & Paik, H.-D.
- Affiliations
- Konkuk University (KR)
- Corresponding author
- Paik, Hyun-Dong
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2020-02-12
- Volume
- 21
- Issue
- 4
- Pages
- 1227
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC7072984
- OpenAlex
- W3006142293
- Cited by
- 86
- References
- 40
- Citations by year
- 2026: 13 · 2025: 17 · 2024: 8 · 2023: 13 · 2022: 16 · 2021: 8 · 2020: 10 · 2019: 1
- FWCI
- 7.62
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Konkuk University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Quality and Safety Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Keywords
- Lactobacillus buchneri, probiotic properties, neuroprotection, Korean fermented foods, Lactobacillus fermentum, Lactobacillus delbrueckii, heat-killed bacteria, conditioned medium, antioxidant activity, MPP+ neurotoxicity, SH-SY5Y cells, HT-29 cells, brain-derived neurotrophic factor, Bax/Bcl-2 ratio, functional foods, gut-brain axis
- MeSH terms
- Fermented Foods, Anti-Bacterial Agents, Antioxidants, Bile Acids and Salts, Gastric Juice, Humans, Intestines, Lactobacillus, 1-Methyl-4-phenylpyridinium, Neuroprotective Agents, HT29 Cells, Proto-Oncogene Proteins c-bcl-2, Probiotics, Limosilactobacillus fermentum, Republic of Korea
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