The DOI matches its Crossref record; 80 of the 80 references it cites were found in a registry or an index.
Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application
Open accessAbstract
Cell cultures are very important for testing materials and drugs, and in the examination of cell biology and special cell mechanisms. The most popular models of cell culture are two-dimensional (2D) as monolayers, but this does not mimic the natural cell environment. Cells are mostly deprived of cell–cell and cell–extracellular matrix interactions. A much better in vitro model is three-dimensional (3D) culture. Because many cell lines have the ability to self-assemble, one 3D culturing method is to produce spheroids. There are several systems for culturing cells in spheroids, e.g., hanging drop, scaffolds and hydrogels, and these cultures have their applications in drug and nanoparticles testing, and disease modeling. In this paper we would like to present methods of preparation of spheroids in general and emphasize the most important applications.
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Allgayer, H., Mahapatra, S., Mishra, B., Swain, B., Saha, S., Khanra, S., Kumari, K., Panda, V. K., Malhotra, D., Patil, N. S., et al. (2025) · Molecular Cancer · doi:10.1186/s12943-025-02338-2 · Cited by 127
Brain organoid methodologies to explore mechanisms of disease in progressive multiple sclerosis
Simões-Abade, M. B. C., Patterer, M., Nicaise, A. M., & Pluchino, S. (2024) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2024.1488691 · Cited by 14
The c-Myc/TBX3 Axis Promotes Cellular Transformation of Sarcoma-Initiating Cells
Damerell, V., Ambele, M. A., Salisbury, S., Neumann-Mufweba, A., Durandt, C., Pepper, M. S., & Prince, S. (2022) · Frontiers in Oncology · doi:10.3389/fonc.2021.801691 · Cited by 12
Recreating Human Skin In Vitro: Should the Microbiota Be Taken into Account?
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Metadata
- Authors
- Białkowska, K., Komorowski, P., Bryszewska, M., & Miłowska, K.
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2020-08-28
- Volume
- 21
- Issue
- 17
- Pages
- 6225
- ISSN
- 1422-0067 (ISSN-L)
- OpenAlex
- W3082990138
- Cited by
- 382
- References
- 80
- FWCI
- 11.28
- Type
- journal article
- Language
- en
- Open access
- gold
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- Białkowska, K., Komorowski, P., Bryszewska, M., & Miłowska, K. (2020). Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21176225
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