The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Three-Dimensional Cell Culture: A Breakthrough in Vivo
Open accessAbstract
Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their surroundings in all three dimensions thanks to an artificial environment. Cells grown in a 3D model have proven to be more physiologically relevant and showed improvements in several studies of biological mechanisms like: cell number monitoring, viability, morphology, proliferation, differentiation, response to stimuli, migration and invasion of tumor cells into surrounding tissues, angiogenesis stimulation and immune system evasion, drug metabolism, gene expression and protein synthesis, general cell function and in vivo relevance. 3D culture models succeed thanks to technological advances, including materials science, cell biology and bioreactor design.
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Citations
Cites
Designing materials for biology and medicine
Langer, R., & Tirrell, D. A. (2004) · Nature · doi:10.1038/nature02388 · Cited by 3,198
The third dimension bridges the gap between cell culture and live tissue
Pampaloni, F., Reynaud, E. G., & Stelzer, E. H. K. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2236 · Cited by 2,817
Capturing complex 3D tissue physiology in vitro
Griffith, L. G., & Swartz, M. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1858 · Cited by 2,290
Recent advances in three‐dimensional multicellular spheroid culture for biomedical research
Lin, R., & Chang, H. (2008) · Biotechnology Journal · doi:10.1002/biot.200700228 · Cited by 1,264
Abbott, A. (2003) · Nature · doi:10.1038/424870a · Cited by 1,234
The role of bioreactors in tissue engineering
Martin, I., Wendt, D., & Heberer, M. (2004) · Trends in Biotechnology · doi:10.1016/j.tibtech.2003.12.001 · Cited by 1,215
Progress and opportunities for tissue-engineered skin
MacNeil, S. (2007) · Nature · doi:10.1038/nature05664 · Cited by 1,112
Three-Dimensional Cell Culture Matrices: State of the Art
Lee, J., Cuddihy, M. J., & Kotov, N. A. (2008) · Tissue Engineering Part B: Reviews · doi:10.1089/teb.2007.0150 · Cited by 1,047
High drug attrition rates—where are we going wrong?
Hutchinson, L., & Kirk, R. (2011) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2011.34 · Cited by 517
Molecular pathways mediating mechanical signaling in bone
Rubin, J., Rubin, C., & Jacobs, C. R. (2006) · Gene · doi:10.1016/j.gene.2005.10.028 · Cited by 474
Cited by
Is it time to start transitioning from 2D to 3D cell culture?
Jensen, C., & Teng, Y. (2020) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2020.00033 · Cited by 1,640
Nicolas, J., Magli, S., Rabbachin, L., Sampaolesi, S., Nicotra, F., & Russo, L. (2020) · Biomacromolecules · doi:10.1021/acs.biomac.0c00045 · Cited by 509
Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research
Pinto, B., Henriques, A. C., Silva, P. M. A., & Bousbaa, H. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12121186 · Cited by 440
The Curious Case of the HepG2 Cell Line: 40 Years of Expertise
Arzumanian, V. A., Kiseleva, O. I., & Poverennaya, E. V. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222313135 · Cited by 387
Barbosa, M. A. G., Xavier, C. P. R., Pereira, R. F., Petrikaitė, V., & Vasconcelos, M. H. (2021) · Cancers · doi:10.3390/cancers14010190 · Cited by 285
Metadata
- Authors
- Antoni, D., Burckel, H., Josset, E., & Noel, G.
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2015-03-11
- Volume
- 16
- Issue
- 3
- Pages
- 5517-5527
- ISSN
- 1422-0067 (ISSN-L)
- OpenAlex
- W2024229755
- Cited by
- 990
- References
- 27
- FWCI
- 29.57
- Type
- journal article
- Language
- en
- Open access
- gold
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
