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Cited by 41Metabolomics and Mass Spectrometry StudiesMollusks and Parasites StudiesAdvanced NMR Techniques and Applications

An 1H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail Helix aspersa Mucus

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Vassilev, Nikolay G., Simova, Svetlana D., Dangalov, Miroslav, Velkova, Lyudmila, Atanasov, Venceslav, Dolashki, Aleksandar, Dolashka, Pavlinka·2020·Metabolites
DOI 10.3390/metabo10090360PMC PMC7570211
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Abstract

Metabolic profiling based on 1H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail Helix aspersa. Twenty metabolites were unambiguously identified by 1H, 1D TOCSY, 2D J-resolved, 2D COSY, and 2D HSQC NMR spectra with water suppression. The metabolic profiles of two fractions with low molecular weight (Mw < 1 kDa and Mw < 3 kDa) are very similar. Metabolites with known antioxidant, antibacterial, and antimicrobial activity were detected by NMR metabolic analysis of mucus samples from Helix aspersa. Some of them were confirmed by mass spectrometric analysis. The primary structure of several peptides was identified in low molecular weight fractions (Mw < 1 kDa) by tandem mass spectrometry.

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  • NMR spectroscopy identified free primary amino acids, sugars (glucose and sucrose), fumarate, osmolytes (betaine and choline), organic acids including isovaleric, lactic, tartaric, and acetic, as well as allantoin, glutathione, and antimicrobial peptides in low molecular weight fractions of lyophilized C. aspersum mucus.

    “NMR spectroscopy was used to study metabolic profiles of low molecular weight fractions (<1 kDa and <3 kDa) of lyophilized C. aspersum mucus. Free primary amino acids were identified in all the fractions; sugars (glucose and sucrose); intermediate metabolites (fumarate); osmolytes (betaine and choline); and several organic acids, including isovaleric, lactic, tartaric, and acetic, as well as allan”

    From Chemical Variability and Biological Potential of Cornu aspersum Mucus as a Source for the Development of New Cosmetic and Pharmaceutical Products (2025) · cites this paper as [38]

    The Conclusions list the claimed compounds in the low-molecular-weight mucus fractions. However, Results §2.2 attributes detection of glutathione and identification of the peptides to mass spectrometry, not NMR spectroscopy; the claim overstates what NMR alone identified. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Sorted by citations

Authors
Vassilev, N. G., Simova, S. D., Dangalov, M., Velkova, L., Atanasov, V., Dolashki, A., & Dolashka, P.
Affiliations
Bulgarian Academy of Sciences (BG)
Corresponding author
  • Vassilev, Nikolay G.
  • Dolashka, Pavlinka
ORCID
Venue
Metabolites
Publisher
MDPI AG
Published
2020-09-02
Volume
10
Issue
9
Pages
360
ISSN
2218-1989 (ISSN-L)
DOI
10.3390/metabo10090360
PMC
PMC7570211
OpenAlex
W3082205943
Cited by
41
References
0
Citations by year
2026: 4 · 2025: 8 · 2024: 9 · 2023: 6 · 2022: 11 · 2021: 2 · 2020: 1
FWCI
1.60
Type
journal article
Language
en
Open access
Licence
Locations
  • Metabolites (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Bulgarian National Science Fund (DН01/14/19.12.2016)
  • Bulgarian Ministry of Education and Science (DO1-217/30.11.2018)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Mollusks and Parasites Studies
  • Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
Keywords
Helix aspersa, mucus, metabolite profiling, small peptides, antioxidant activity, antibacterial activity, antimicrobial activity, peptide primary structure

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