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Cited by 117Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyMilk Quality and Mastitis in Dairy Cows

The DOI matches its Crossref record; 76 of the 76 references it cites were found in a registry or an index.

Pasture Feeding Changes the Bovine Rumen and Milk Metabolome

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O’Callaghan, Tom, Vázquez-Fresno, Rosa, Serra-Cayuela, Arnau, Dong, Edison, Mandal, Rupasri, Hennessy, Deirdre, McAuliffe, Stephen, Dillon, Pat, Wishart, David, Stanton, Catherine, Ross, R.·2018·Metabolites
DOI 10.3390/metabo8020027PMC PMC6027121
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Abstract

The purpose of this study was to examine the effects of two pasture feeding systems—perennial ryegrass (GRS) and perennial ryegrass and white clover (CLV)—and an indoor total mixed ration (TMR) system on the (a) rumen microbiome; (b) rumen fluid and milk metabolome; and (c) to assess the potential to distinguish milk from different feeding systems by their respective metabolomes. Rumen fluid was collected from nine rumen cannulated cows under the different feeding systems in early, mid and late lactation, and raw milk samples were collected from ten non-cannulated cows in mid-lactation from each of the feeding systems. The microbiota present in rumen liquid and solid portions were analysed using 16S rRNA gene sequencing, while 1H-NMR untargeted metabolomic analysis was performed on rumen fluid and raw milk samples. The rumen microbiota composition was not found to be significantly altered by any feeding system in this study, likely as a result of a shortened adaptation period (two weeks’ exposure time). In contrast, feeding system had a significant effect on both the rumen and milk metabolome. Increased concentrations of volatile fatty acids including acetic acid, an important source of energy for the cow, were detected in the rumen of TMR and CLV-fed cows. Pasture feeding resulted in significantly higher concentrations of isoacids in the rumen. The ruminal fluids of both CLV and GRS-fed cows were found to have increased concentrations of p-cresol, a product of microbiome metabolism. CLV feeding resulted in increased rumen concentrations of formate, a substrate compound for methanogenesis. The TMR feeding resulted in significantly higher rumen choline content, which contributes to animal health and milk production, and succinate, a product of carbohydrate metabolism. Milk and rumen-fluids were shown to have varying levels of dimethyl sulfone in each feeding system, which was found to be an important compound for distinguishing between the diets. CLV feeding resulted in increased concentrations of milk urea. Milk from pasture-based feeding systems was shown to have significantly higher concentrations of hippuric acid, a potential biomarker of pasture-derived milk. This study has demonstrated that 1H-NMR metabolomics coupled with multivariate analysis is capable of distinguishing both rumen-fluid and milk derived from cows on different feeding systems, specifically between indoor TMR and pasture-based diets used in this study.

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Crossref · 10.3390/metabo8020027

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Figure 1 Bar chart demonstrating breakdown of abundances of different phyla in rumen fluid (A) and rumen solid (B) portions of rumen microbiota.
Figure 1 Bar chart demonstrating breakdown of abundances of different phyla in rumen fluid (A) and rumen solid (B) portions of rumen microbiota.CC BY · PubMed Central
Figure 2 Bar chart demonstrating breakdown of abundances of different genera in rumen fluid (A) and rumen solid (B) portions of rumen microbiota. (Genera present at > 1% abundance in at least 1 sample).
Figure 2 Bar chart demonstrating breakdown of abundances of different genera in rumen fluid (A) and rumen solid (B) portions of rumen microbiota. (Genera present at > 1% abundance in at least 1 sample).CC BY · PubMed Central
Figure 3 Hierarchical clustering analysis (heatmap) of average rumen fluid metabolites from lactating dairy cows fed diets consisting of total mixed ration (TMR), perennial ryegrass (GRS), or perennial ryegrass and white clover (CLV), as determined by 1H-NMR. The degree of positive and negative correlation of metabolite to dietary system is indicated by +1 (red) to −1 (blue).
Figure 3 Hierarchical clustering analysis (heatmap) of average rumen fluid metabolites from lactating dairy cows fed diets consisting of total mixed ration (TMR), perennial ryegrass (GRS), or perennial ryegrass and white clover (CLV), as determined by 1H-NMR. The degree of positive and negative correlation of metabolite to dietary system is indicated by +1 (red) to −1 (blue).CC BY · PubMed Central
Figure 4 Variable importance plot (VIP) which shows the compounds primarily responsible for separation of rumen fluid metabolomes from cows fed diets consisting of total mixed ration (TMR), perennial ryegrass (GRS), or perennial ryegrass and white clover (CLV), as determined by PLS-DA.
Figure 4 Variable importance plot (VIP) which shows the compounds primarily responsible for separation of rumen fluid metabolomes from cows fed diets consisting of total mixed ration (TMR), perennial ryegrass (GRS), or perennial ryegrass and white clover (CLV), as determined by PLS-DA.CC BY · PubMed Central
Figure 5 Hierarchical clustering analysis (heatmap) of average raw milk metabolites from mid-lactation cows fed diets consisting of total mixed ration (TMR), perennial ryegrass (GRS), or perennial ryegrass and white clover (CLV), as determined by 1H-NMR. The degree of positive and negative correlation of metabolite to dietary system is indicated by +1 (red) to −1 (blue).
Figure 5 Hierarchical clustering analysis (heatmap) of average raw milk metabolites from mid-lactation cows fed diets consisting of total mixed ration (TMR), perennial ryegrass (GRS), or perennial ryegrass and white clover (CLV), as determined by 1H-NMR. The degree of positive and negative correlation of metabolite to dietary system is indicated by +1 (red) to −1 (blue).CC BY · PubMed Central
Figure 6 Partial least square discriminant analysis (PLS-DA) score plot of mid-lactation raw milk metabolome of cows fed diets consisting of total mixed ration (TMR), perennial ryegrass (GRS), or perennial ryegrass and white clover (CLV), as determined by 1H-NMR. The shaded ellipses represent the 95% confidence interval estimated from the score.
Figure 6 Partial least square discriminant analysis (PLS-DA) score plot of mid-lactation raw milk metabolome of cows fed diets consisting of total mixed ration (TMR), perennial ryegrass (GRS), or perennial ryegrass and white clover (CLV), as determined by 1H-NMR. The shaded ellipses represent the 95% confidence interval estimated from the score.CC BY · PubMed Central

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Research works printed but not linked: 2

  1. Sundekilde, 2013, NMR-based milk metabolomics, Metabolites, 3, 204, 10.3390/metabo3020204 · DOI
  2. Prache, S., Priolo, A., Tournadre, H., Jailler, R., Dubroeucq, H., Micol, D., Martin, B., Durand, J., Emile, J., and Huyghe, C. (2002, January 27–30). Traceability of Grass-Feeding by Quantifying the Signature of Carotenoid Pigments in Herbivores Meat, Milk and Cheese, Multi-function grasslands: Quality forages, animal products and landscapes. Proceedings of the 19th General Meeting of the European Grassland Federation, La Rochelle, France.

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Authors
O’Callaghan, T., Vázquez-Fresno, R., Serra-Cayuela, A., Dong, E., Mandal, R., Hennessy, D., McAuliffe, S., Dillon, P., Wishart, D., Stanton, C., & Ross, R.
Affiliations
  • Teagasc - The Irish Agriculture and Food Development Authority (IE)
  • The Metabolomics Innovation Centre (TMIC), University of Alberta, Edmonton, AB T6G1C9, Canada
  • University of Alberta (CA)
  • Queen's University Belfast (GB)
Corresponding author
Ross, R.
ORCID
Venue
Metabolites
Publisher
MDPI AG
Published
2018-04-06
Volume
8
Issue
2
Pages
27
ISSN
2218-1989 (ISSN-L)
DOI
10.3390/metabo8020027
PMC
PMC6027121
OpenAlex
W2795936251
Cited by
117
References
76
Citations by year
2026: 8 · 2025: 15 · 2024: 10 · 2023: 8 · 2022: 18 · 2021: 22 · 2020: 20 · 2019: 13 · 2018: 3
FWCI
24.50
Type
journal article
Language
en
Open access
Licence
Locations
  • Metabolites (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Cork Open Research Archive (University College Cork, Ireland) (publishedVersion, other-oa)
  • Arrow@dit (Dublin Institute of Technology) (acceptedVersion)
  • Arrow@dit (Dublin Institute of Technology) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • T-Stór (Teagasc) (submittedVersion, cc-by-nc-sa)
Funders
  • Teagasc
  • Science Foundation Ireland (SFI/12/RC/2273)
  • Dairy Levy (Dairy Research Ireland)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Milk Quality and Mastitis in Dairy Cows
Keywords
pasture grazing, dairy cattle, rumen microbiome, milk metabolome, rumen fluid, total mixed ration, perennial ryegrass, white clover, volatile fatty acids, p-cresol, formate, choline, succinic acid, dimethyl sulfone, hippuric acid, milk urea nitrogen, 1H NMR metabolomics

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