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Lipidomic Profiling of Murine Macrophages Treated with Fatty Acids of Varying Chain Length and Saturation Status
Open accessAbstract
Macrophages are abundant within adipose tissue depots where they are exposed to fatty acids, leading to lipid accumulation. Herein, we have determined the effects of various fatty acids on the macrophage lipidome. Using targeted mass-spectrometry, we were able to detect 641 individual lipid species in primary murine macrophages treated with a variety of saturated fatty acids and an un-saturated fatty acid, either alone or in combination. The most pronounced effects were observed for the long-chain saturated fatty acid palmitate, which increased the total abundance of numerous classes of lipids. While other medium- and long-chain saturated fatty acids, as well as the long-chain unsaturated fatty acid, had less pronounced effects on the total abundance of specific lipid classes, all fatty acids induced marked alterations in the abundance of numerous lipid species within given lipid classes. Fatty acid treatment markedly altered overall phospholipid saturation status; these effects were most pronounced for phosphatidylcholine and ether-phosphatidylcholine lipid species. Finally, treatment of macrophages with either palmitate or stearate in combination with oleate prevented many of the changes that were observed in macrophages treated with palmitate or stearate alone. Collectively, our results reveal substantial and specific remodelling of the macrophage lipidome following treatment with fatty acids.
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Altered lipid metabolism and extrinsic supplementation with various lipid substrates have a major impact on microglial reprogramming.
“Altered lipid metabolism and extrinsic supplementation with various lipid substrates both have a major impact on microglial reprogramming [60,61,62,63,64].”
From Lipoprotein Lipase Regulates Microglial Lipid Droplet Accumulation (2021) · cites this paper as [61]
The Discussion (section 7; page not provided) reports that supplying different fatty acids markedly alters the lipidome of murine bone-marrow-derived macrophages. The Introduction (section 1; page not provided) also links lipid uptake and metabolism to inflammatory signaling in adipose-tissue macrophages. Neither passage establishes an impact on microglial reprogramming, which is the cell type and outcome claimed here. (LLM, conf=0.86)
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Metadata
- Authors
- Huynh, K., Pernes, G., Mellett, N. A., Meikle, P. J., Murphy, A. J., & Lancaster, G. I.
- Affiliations
- Baker Heart and Diabetes Institute (AU)
- Corresponding author
- Lancaster, Graeme I.
- ORCID
- Venue
- Metabolites
- Publisher
- MDPI AG
- Published
- 2018-04-23
- Volume
- 8
- Issue
- 2
- Pages
- 29
- ISSN
- 2218-1989 (ISSN-L)
- PMC
- PMC6027068
- OpenAlex
- W2800410090
- Cited by
- 27
- References
- 29
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 6 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 8 · 2018: 2
- FWCI
- 1.40
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Metabolites (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Minerva Access (University of Melbourne) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Dementia Australia
- Baker Heart and Diabetes Institute
- National Health and Medical Research Council (1042095)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Keywords
- fatty acid chain length, palmitate, stearate, oleate, phosphatidylcholine, lipid remodeling, adipose tissue macrophages, lipid accumulation
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