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Cited by 15Gut microbiota and healthGastrointestinal motility and disordersAdipokines, Inflammation, and Metabolic Diseases

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Effect of Oral Administration of Polyethylene Glycol 400 on Gut Microbiota Composition and Diet-Induced Obesity in Mice

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Ishibashi, Riko, Matsuhisa, Rio, Nomoto, Mio, Chudan, Seita, Nishikawa, Miyu, Tabuchi, Yoshiaki, Ikushiro, Shinichi, Nagai, Yoshinori, Furusawa, Yukihiro·2023·Microorganisms
DOI 10.3390/microorganisms11081882PMC PMC10456793
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Abstract

Polyethylene glycol (PEG) is a commonly used dispersant for oral administration of hydrophobic agents. PEG is partly absorbed in the small intestine, and the unabsorbed fraction reaches the large intestine; thus, oral administration of PEG may impact the gut microbial community. However, to the best of our knowledge, no study evaluated the effects of PEG on gut commensal bacteria. Herein, we aimed to determine whether oral administration of PEG modifies the gut microbiota. Administration of PEG400 and PEG4000 altered gut microbial diversity in a concentration-dependent manner. Taxonomic analysis revealed that Akkermansia muciniphila and particularly Parabacteroides goldsteinii were overrepresented in mice administered with 40% PEG. PEG400 administration ameliorated the high-fat diet (HFD)-induced obesity and adipose tissue inflammation. Fecal microbiome transplantation from PEG400-administered donors counteracted the HFD-induced body and epididymal adipose tissue weight gain, indicating that PEG400-associated bacteria are responsible for the anti-obesity effect. Conversely, carboxymethyl cellulose, also used as a dispersant, did not affect the abundance of these two bacterial species or HFD-induced obesity. In conclusion, we demonstrated that oral administration of a high concentration of PEG400 (40%) alters the gut microbiota composition and ameliorates HFD-induced obesity.

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Metadata

Authors
Ishibashi, R., Matsuhisa, R., Nomoto, M., Chudan, S., Nishikawa, M., Tabuchi, Y., Ikushiro, S., Nagai, Y., & Furusawa, Y.
Affiliations
  • Toyama Prefectural University (JP)
  • University of Toyama (JP)
Corresponding author
Furusawa, Yukihiro
ORCID
Venue
Microorganisms
Publisher
MDPI AG
Published
2023-07-26
Volume
11
Issue
8
Pages
1882
ISSN
2076-2607 (ISSN-L)
DOI
10.3390/microorganisms11081882
PMC
PMC10456793
OpenAlex
W4385317911
Cited by
15
References
2
Citations by year
2026: 6 · 2025: 7 · 2024: 2
FWCI
1.34
Type
journal article
Language
en
Open access
Licence
Locations
  • Microorganisms (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • JSPS KAKENHI (20K05929)
  • Takeda Science Foundation (20K05929)
  • Tojuro Iijima Foundation for Food Science and Technology (20K05929)
  • Kanamori Foundation (20K05929)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
Keywords
polyethylene glycol 400, gut microbiota, diet-induced obesity, high-fat diet, Akkermansia muciniphila, Parabacteroides goldsteinii, adipose tissue inflammation, fecal microbiota transplantation, carboxymethyl cellulose, dispersants, oral administration, hydrophobic drug delivery, epididymal adipose tissue

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