The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection
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Lactobacillus fermentum UCO-979C (Lf979C) beneficially modulates the cytokine response of gastric epithelial cells and macrophages after Helicobacter pylori infection in vitro. Nevertheless, no in vivo studies were performed with this strain to confirm its beneficial immunomodulatory effects. This work evaluated whether Lf979C improves protection against H. pylori infection in mice by modulating the innate immune response. In addition, we evaluated whether its exopolysaccharide (EPS) was involved in its beneficial effects. Lf979C significantly reduced TNF-α, IL-8, and MCP-1 and augmented IFN-γ and IL-10 in the gastric mucosa of H. pylori-infected mice. The differential cytokine profile induced by Lf979C in H. pylori-infected mice correlated with an improved reduction in the pathogen gastric colonization and protection against inflammatory damage. The purified EPS of Lf979C reduced IL-8 and enhanced IL-10 levels in the gastric mucosa of infected mice, while no effect was observed for IFN-γ. This work demonstrates for the first time the in vivo ability of Lf979C to increase resistance against H. pylori infection by modulating the gastric innate immune response. In addition, we advanced knowledge of the mechanisms involved in the beneficial effects of Lf979C by demonstrating that its EPS is partially responsible for its immunomodulatory effect.
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EPS secreted by Lim. fermentum UCO-979C was able to reduce H. pylori adhesion (~30%) in both in vitro and in vivo studies, and was also involved in ameliorating immune response in H. pylori infections.
“EPS secreted by Lim. fermentum UCO-979C was able to reduce H. pylori adhesion (~30%) in both in vitro and in vivo studies, being also involved in ameliorating the immune response in H. pylori infections [154].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [154]
The Discussion reports an approximately 30% reduction in H. pylori adhesion to AGS cells in vitro, and Results §3.5 reports altered inflammatory cytokine levels in infected mice treated with UCO-979C EPS. The claim extends the adhesion reduction to in vivo studies, but the Discussion says the EPS was unable to reduce H. pylori counts in vivo. Page numbers are unavailable in our copy. (LLM, conf=0.90)
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Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
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Self-citation
- Authors
- Garcia-Castillo, V., Marcial, G., Albarracín, L., Tomokiyo, M., Clua, P., Takahashi, H., Kitazawa, H., Garcia-Cancino, A., & Villena, J.
- Affiliations
- Laboratory of Bacterial Pathogenicity, Faculty of Biological Sciences, University of Concepcion, Concepcion Bio Bio 4030000, Chile
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- National University of Tucumán (AR)
- Tohoku University (JP)
- Corresponding author
- Kitazawa, Haruki
- Villena, Julio
- ORCID
- Venue
- Microorganisms
- Publisher
- MDPI AG
- Published
- 2020-03-27
- Volume
- 8
- Issue
- 4
- Pages
- 479
- ISSN
- 2076-2607 (ISSN-L)
- PMC
- PMC7232353
- OpenAlex
- W3013072219
- Cited by
- 34
- References
- 65
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 6 · 2023: 5 · 2022: 6 · 2021: 8
- FWCI
- 3.08
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Microorganisms (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-sa)
- Funders
- Agencia Nacional de Promoción Científica y Tecnológica (PICT-2016-0410)
- Japan Society for the Promotion of Science (19H00965, 16H05019, 16K15028, JSPS Core-to-Core Program Advanced Research Networks)
- NARO Bio-oriented Technology Research Advancement Institution (No. 01002A)
- Comisión Nacional de Investigación Científica y Tecnológica (National Doctorate Grant 21150603)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Medicine › Surgery › Helicobacter pylori-related gastroenterology studies
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus fermentum, exopolysaccharides, Helicobacter pylori infection, immunomodulation, innate immunity, gastric mucosa, cytokine profile, IL-10, interleukin-8, interferon-gamma, TNF-α, MCP-1, gastric colonization, inflammatory damage, probiotic protection
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