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Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn
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The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn) characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds.
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“The application of metabolomics to foods has allowed the identification of a large variety of novel food constituents that are either naturally present in the food species or formed during food processing (65,173,174).”
From The food metabolome: a window over dietary exposure (2014) · cites this paper as [174]
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Metadata
- Authors
- Mena, P., Calani, L., Dall'Asta, C., Galaverna, G., García-Viguera, C., Bruni, R., Crozier, A., & Del Rio, D.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- University of Parma (IT)
- Department of Food Science, University of Parma, Parma 43125, Italy
- Phytochemistry Laboratory, Department of Food Science and Technology, CEBAS-CSIC, Murcia 30100, Spain
- University of Glasgow (GB)
- The φ2 Laboratory of Phytochemicals in Physiology, Department of Food Science, University of Parma, Parma 43125, Italy
- ORCID
- Venue
- Molecules
- Publisher
- MDPI AG
- Published
- 2012-12-13
- Volume
- 17
- Issue
- 12
- Pages
- 14821-14840
- ISSN
- 1420-3049 (ISSN-L)
- PMC
- PMC6268091
- OpenAlex
- W2036187997
- Cited by
- 321
- References
- 50
- Citations by year
- 2026: 19 · 2025: 18 · 2024: 20 · 2023: 25 · 2022: 33 · 2021: 33 · 2020: 35 · 2019: 32 · 2018: 21 · 2017: 21 · 2016: 24 · 2015: 22 · 2014: 12 · 2013: 6
- FWCI
- 11.91
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Molecules (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (acceptedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Keywords
- pomegranate juice, Punica granatum, polyphenols, phenolic acids, flavonoids, ellagitannins, anthocyanins, hydrolysable tannins, phenolic profile, linear ion trap mass spectrometry, compound identification, fingerprinting
- MeSH terms
- Anthocyanins, Beverages, Chromatography, High Pressure Liquid, Plant Extracts, Mass Spectrometry, Lignans, Lythraceae, Hydrolyzable Tannins, Polyphenols
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