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Rapid and Comprehensive Evaluation of (Poly)phenolic Compounds in Pomegranate (Punica granatum L.) Juice by UHPLC-MSn
Open accessAbstract
The comprehensive identification of phenolic compounds in food and beverages is a crucial starting point for assessing their biological, nutritional, and technological properties. Pomegranate (Punica granatum L.) has been described as a rich source of (poly)phenolic components, with a broad array of different structures (phenolic acids, flavonoids, and hydrolyzable tannins) and a quick, high throughput, and accurate screening of its complete profile is still lacking. In the present work, a method for UHPLC separation and linear ion trap mass spectrometric (MSn) characterization of pomegranate juice phenolic fraction was optimized by comparing several different analytical conditions. The best solutions for phenolic acids, anthocyanins, flavonoids, and ellagitannins have been delineated and more than 70 compounds have been identified and fully characterized in less than one hour total analysis time. Twenty-one compounds were tentatively detected for the first time in pomegranate juice. The proposed fingerprinting approach could be easily translated to other plant derived food extracts and beverages containing a wide array of phytochemical compounds.
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“The application of metabolomics to foods has allowed the identification of a large variety of novel food constituents that are either naturally present in the food species or formed during food processing (65,173,174).”
From The food metabolome: a window over dietary exposure (2014) · cites this paper as [174]
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Del Rio, D., Rodriguez-Mateos, A., Spencer, J. P. E., Tognolini, M., Borges, G., & Crozier, A. (2013) · Antioxidants & Redox Signaling · doi:10.1089/ars.2012.4581 · Cited by 2,601
Gil, M. I., Tomás-Barberán, F. A., Hess-Pierce, B. M., Holcroft, D. M., & Kader, A. A. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf000404a · Cited by 2,365
Fischer, U. A., Carle, R., & Kammerer, D. R. (2011) · Food Chemistry · doi:10.1016/j.foodchem.2010.12.156 · Cited by 876
Quantitative Analysis of Flavan-3-ols in Spanish Foodstuffs and Beverages
de Pascual‐Teresa, S., Santos‐Buelga, C., & Rivas-Gonzalo, J. C. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf000549h · Cited by 435
Sawada, Y., Akiyama, K., Sakata, A., Kuwahara, A., Otsuki, H., Sakurai, T., Saito, K., & Hirai, M. Y. (2008) · Plant and Cell Physiology · doi:10.1093/pcp/pcn183 · Cited by 310
Mena, P. R., García‐Viguera, C., Navarro-Rico, J., Moreno, D. A., Bartual, J., Saura, D., & Martí, N. (2011) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.4411 · Cited by 279
Applications of liquid chromatography–mass spectrometry for food analysis
Stefano, V. A. D., Avellone, G., Bongiorno, D., Cunsolo, V., Muccilli, V., Sforza, S., Dossena, A., Drahos, L., & Vékey, K. (2012) · Journal of Chromatography A · doi:10.1016/j.chroma.2012.04.023 · Cited by 221
Quantitative determination of the polyphenolic content of pomegranate peel
Nasr, C. B., Ayed, N., & Metche, M. (1996) · European Food Research and Technology · doi:10.1007/bf01231077 · Cited by 201
Eklund, P., Backman, M. J., Krönberg, L., Smeds, A. I., & Sjöholm, R. E. (2007) · Journal of Mass Spectrometry · doi:10.1002/jms.1276 · Cited by 170
Chemistry and Biology of Ellagitannins
Quideau, S. (2009) · WORLD SCIENTIFIC eBooks · doi:10.1142/6795 · Cited by 163
Hukkanen, A. T., Kokko, H. I., Buchala, A. J., McDougall, G. J., Stewart, D., Kärenlampi, S. O., & Karjalainen, R. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf063452p · Cited by 163
Changes in pomegranate juice pigmentation during ripening
Gil, M. I., García‐Viguera, C., Artés, F., & Tomás-Barberán, F. A. (1995) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740680113 · Cited by 151
Gasperotti, M., Masuero, D., Vrhovšek, U., Guella, G., & Mattivi, F. (2010) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf904543w · Cited by 146
Li, H., Xu, H., Liu, X., He, W., Yuan, F., Hou, Z., & Gao, Y. (2010) · Food Research International · doi:10.1016/j.foodres.2010.05.023 · Cited by 141
Antioxidant Activity of Isolated Ellagitannins from Red Raspberries and Cloudberries
Kähkönen, M., Kylli, P., Ollilainen, V., Salminen, J., & Heinonen, M. (2012) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf203431g · Cited by 129
Romani, A., Campo, M., & Pinelli, P. (2011) · Food Chemistry · doi:10.1016/j.foodchem.2011.07.009 · Cited by 129
Influence of packaging material on pomegranate juice colour and bioactive compounds, during storage
Pérez-Vicente, A., Serrano, P., Abellán, P., & García‐Viguera, C. (2004) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.1721 · Cited by 118
Phenolic profile of Sercial and Tinta Negra Vitis vinifera L. grape skins by HPLC–DAD–ESI-MSn
Perestrelo, R., Lu, Y., Santos, S. A. O., Silvestre, A. J. D., Neto, C. P., Câmara, J. S., & Rocha, S. M. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.04.102 · Cited by 117
de Rijke, E., Zappey, H. W., Ariese, F., Gooijer, C., & Brinkman, U. A. T. (2002) · Journal of Chromatography A · doi:10.1016/s0021-9673(02)01868-x · Cited by 116
Antioxidant properties of gallocatechin and prodelphinidins from pomegranate peel
Plumb, G. W., de Pascual‐Teresa, S., Santos‐Buelga, C., Rivas-Gonzalo, J. C., & Williamson, G. (2002) · Redox Report · doi:10.1179/135100002125000172 · Cited by 109
Borges, G., Mullen, W., & Crozier, A. (2010) · Food & Function · doi:10.1039/c0fo00008f · Cited by 109
Liu, R., Ye, M., Guo, H., Bi, K., & Guo, D. (2005) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.1936 · Cited by 108
Natural Hypolignification Is Associated with Extensive Oligolignol Accumulation in Flax Stems
Huis, R., Morreel, K., Fliniaux, O., Lucau‐Danila, A., Fénart, S., Grec, S., Neutelings, G., Chabbert, B., Mesnard, F., Boerjan, W., et al. (2012) · PLANT PHYSIOLOGY · doi:10.1104/pp.111.192328 · Cited by 101
Pomegranate varietal wines: Phytochemical composition and quality parameters
Mena, P. R., Gironés‐Vilaplana, A., Martí, N., & García‐Viguera, C. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2011.12.079 · Cited by 98
Barry, K., Davies, N. W., & Mohammed, C. L. (2001) · Phytochemical Analysis · doi:10.1002/pca.548 · Cited by 94
Sun, J., Lin, L., & Chen, P. C. (2012) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.6209 · Cited by 93
Boulekbache‐Makhlouf, L., Meudec, E., Chibane, M., Mazauric, J. P., Slimani, S., Henry, M., Cheynier, V., & Madani, K. (2010) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf1029509 · Cited by 93
Negative ion ESI–MS analysis of natural yellow dye flavonoids—An isotopic labelling study
McNab, H., Ferreira, E. S. B., Hulme, A. N., & Quye, A. (2008) · International Journal of Mass Spectrometry · doi:10.1016/j.ijms.2008.05.039 · Cited by 88
Wang, Y., Yang, L., He, Y., Wang, C., Welbeck, E. W., Bligh, S. W. A., & Wang, Z. (2008) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.3536 · Cited by 87
Fischer, U. A., Dettmann, J., Carle, R., & Kammerer, D. R. (2011) · European Food Research and Technology · doi:10.1007/s00217-011-1560-3 · Cited by 83
Zywicki, B., Reemtsma, T., & Jekel, M. R. (2002) · Journal of Chromatography A · doi:10.1016/s0021-9673(02)00883-x · Cited by 80
Zhang, Y., Krueger, D. A., Durst, R. W., Lee, R., Wang, D., Seeram, N. P., & Heber, D. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf803172e · Cited by 73
Vrhovšek, U., Guella, G., Gasperotti, M., Pojer, E., Zancato, M., & Mattivi, F. (2012) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf2052256 · Cited by 73
Combinatory Effect of Thermal Treatment and Blending on the Quality of Pomegranate Juices
Mena, P. R., Martí, N., Saura, D., Valero, M., & García‐Viguera, C. (2012) · Food and Bioprocess Technology · doi:10.1007/s11947-012-0961-z · Cited by 72
Bonzanini, F., Bruni, R., Palla, G., Serlataite, N., & Caligiani, A. (2009) · Food Chemistry · doi:10.1016/j.foodchem.2009.04.057 · Cited by 68
Sanz, M., de Simón, B. F., Esteruelas, E., Muñoz, A. M., Cadahía, E., Hernández, T., Estrella, I., & Pinto, E. (2011) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf1042932 · Cited by 62
HPLC–PDA–MS fingerprinting to assess the authenticity of pomegranate beverages
Borges, G., & Crozier, A. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.05.108 · Cited by 57
Filigenzi, M. S., Ehrke, N., Aston, L. S., & Poppenga, R. H. (2011) · Food Additives & Contaminants Part A · doi:10.1080/19440049.2011.604796 · Cited by 57
Petroviciu, I., Berghe, I. V., Cretu, I., Albu, F., & Medvedovici, A. V. (2011) · Journal of Cultural Heritage · doi:10.1016/j.culher.2011.05.004 · Cited by 55
Li, H., Qiu, J., Chen, F., Lv, X., Fu, C., Zhao, D., Hua, X., & Zhao, Q. L. (2011) · Molecular Biology Reports · doi:10.1007/s11033-011-1061-2 · Cited by 54
Fruit quality of Italian pomegranate (Punica granatum L.) autochthonous varieties
Cristofori, V., Caruso, D., Latini, G., Dell’Agli, M., Cammilli, C., Rugini, E., Bignami, C., & Muleo, R. (2010) · European Food Research and Technology · doi:10.1007/s00217-010-1390-8 · Cited by 49
Mulabagal, V., & Calderón, Á. I. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.02.123 · Cited by 38
Regos, I., & Treutter, D. (2010) · Journal of Chromatography A · doi:10.1016/j.chroma.2010.07.075 · Cited by 38
Three-step HPLC–ESI-MS/MS procedure for screening and identifying non-target flavonoid derivatives
Rak, G., Fodor, P., & Abrankó, L. (2009) · International Journal of Mass Spectrometry · doi:10.1016/j.ijms.2009.11.008 · Cited by 37
Fischer, U. A., Jaksch, A. V., Carle, R., & Kammerer, D. R. (2011) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf203598m · Cited by 35
Barry, K., Pearce, R. B., & Mohammed, C. (2000) · Forest Pathology · doi:10.1046/j.1439-0329.2000.00206.x · Cited by 30
Sentandreu, E., Navarro, J. L., & Sendra, J. M. (2010) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf101978z · Cited by 28
Sources and Health Effects of Dietary Ellagitannins
Törrönen, R. (2009) · WORLD SCIENTIFIC eBooks · doi:10.1142/9789812797414_0008 · Cited by 27
Electron-impact and glow-discharge ionization LC–MS analysis of green tea tincture
Venzie, J. L., Castro, J., Krishna, M. V. B., Nelson, D. M., & Marcus, R. K. (2006) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-006-0934-z · Cited by 19
Selecting a Suitable LC Column for Pharmaceutical Separations using a Column Characterisation System
Haghedooren, E., Janssens, T., Nijs, R. D., Park, S. K., Farkas, E., Dragović, S., Noszál, B., Hoogmartens, J., & Adams, E. (2009) · Journal of Liquid Chromatography & Related Technologies · doi:10.1080/10826070902766553 · Cited by 4
Flagged first, then by citations
Research works printed but not linked: 1
- Mena, 2011, “Pomegranate fruit for health promotion: Myths and realities” In Antioxidant Properties of Crops III, Funct. Plant Sci. Biotechnol., 5, 33
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Sorted by citations
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Ambigaipalan, P., de Camargo, A. C., & Shahidi, F. (2016) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.6b02950 · Cited by 281
Metadata
- Authors
- Mena, P., Calani, L., Dall'Asta, C., Galaverna, G., García-Viguera, C., Bruni, R., Crozier, A., & Del Rio, D.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- University of Parma (IT)
- Department of Food Science, University of Parma, Parma 43125, Italy
- Phytochemistry Laboratory, Department of Food Science and Technology, CEBAS-CSIC, Murcia 30100, Spain
- University of Glasgow (GB)
- The φ2 Laboratory of Phytochemicals in Physiology, Department of Food Science, University of Parma, Parma 43125, Italy
- ORCID
- Venue
- Molecules
- Publisher
- MDPI AG
- Published
- 2012-12-13
- Volume
- 17
- Issue
- 12
- Pages
- 14821-14840
- ISSN
- 1420-3049 (ISSN-L)
- PMC
- PMC6268091
- OpenAlex
- W2036187997
- Cited by
- 321
- References
- 50
- Citations by year
- 2026: 19 · 2025: 18 · 2024: 20 · 2023: 25 · 2022: 33 · 2021: 33 · 2020: 35 · 2019: 32 · 2018: 21 · 2017: 21 · 2016: 24 · 2015: 22 · 2014: 12 · 2013: 6
- FWCI
- 11.91
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Molecules (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (acceptedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Keywords
- pomegranate juice, Punica granatum, polyphenols, phenolic acids, flavonoids, ellagitannins, anthocyanins, hydrolysable tannins, phenolic profile, linear ion trap mass spectrometry, compound identification, fingerprinting
- MeSH terms
- Anthocyanins, Beverages, Chromatography, High Pressure Liquid, Plant Extracts, Mass Spectrometry, Lignans, Lythraceae, Hydrolyzable Tannins, Polyphenols
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