Design of Liposomes Carrying HelixComplex Snail Mucus: Preliminary Studies
Open accessAbstract
In recent decades liposomes have been used in different field thanks to their ability to act as a vehicle for a wide range of biomolecules, their great versatility and their easy production. The aim of this study was to evaluate liposomes as a vehicle for the actives present in the HelixComplex (HC) snail mucus for topical delivery. Liposomes composed of a mixture of phosphatidylcholine, cholesterol and octadecylamine were prepared with and without HC (empty liposomes) and their biological efficacy was tested by evaluating cell viability and migration. HC-loaded liposomes (LHC) were stable throughout 60 days of observation, and showed interesting effects on wound healing reconstitution. In particular, we observed that 25 µg/mL LHC were already able to induce a higher cell monolayer reconstitution in comparison to the untreated samples and HC treated samples after only 4 h (28% versus 10% and 7%, p = 0.03 and p= 0.003, respectively). The effect was more evident at 24 h in comparison with the untreated control (54% versus 21.2% and 41.6%, p = 0.006 and p = NS, respectively). These results represent a preliminary, but promising, novelty in the delivery strategy of the actives present in the HelixComplex mucus.
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Studies confirm better local bioavailability of snail mucus in liposomes.
“In addition, there are studies that confirm a better local bioavailability of snail mucus in liposomes [101], which can be a strong pharmaceutical factor in the development of investigational medicinal products of snail mucus for topical application.”
From Chemical Variability and Biological Potential of Cornu aspersum Mucus as a Source for the Development of New Cosmetic and Pharmaceutical Products (2025) · cites this paper as [101]
The Results and Discussion (§3.3) report better tissue-repair activity when snail mucus was loaded in liposomes than when it was applied alone. But the Conclusions (§4) describe improved cellular uptake as a proposed explanation and say in vivo tissue distribution still needs confirmation. The study supports improved in vitro activity, not a confirmed improvement in local bioavailability. (LLM, conf=0.86)
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- Authors
- Alogna, A., Gentili, V., Trapella, C., Hallan, S. S., Sguizzato, M., Strazzabosco, G., Fernández, M., Cortesi, R., Rizzo, R., & Bortolotti, D.
- Affiliations
- University of Ferrara (IT)
- Corresponding author
- Alogna, Andrea
- Cortesi, Rita
- ORCID
- Venue
- Molecules
- Publisher
- MDPI AG
- Published
- 2021-08-04
- Volume
- 26
- Issue
- 16
- Pages
- 4709
- ISSN
- 1420-3049 (ISSN-L)
- PMC
- PMC8399803
- OpenAlex
- W3189682058
- Cited by
- 14
- References
- 23
- Citations by year
- 2026: 3 · 2025: 4 · 2023: 3 · 2022: 4
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Update notices
- correction (2021-08-04)
- Open access
- gold
- doi.org
- Licence
- Locations
- Molecules (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Institutional Research Information System University of Ferrara (University of Ferrara) (submittedVersion, cc-by)
- Funders
- PON “Research and Innovation” 2014-2020 (ARS01_01163)
- University of Ferrara (FAR2020, FAR2019)
- Chamber of Commerce of Ferrara (CCIAA2018, CCIAA2019)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- liposomes, snail mucus, topical drug delivery, wound healing, cell migration, cell viability, phosphatidylcholine, cholesterol, octadecylamine, liposome stability
- MeSH terms
- Animals, Cell Line, Fibroblasts, Freeze Fracturing, Humans, Lipids, Liposomes, Mucus, Snails, Spectrophotometry, Infrared, Wound Healing, Cell Death
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