Storage Stability of Blood Samples for miRNAs in Glycosylated Extracellular Vesicles
Open accessAbstract
Extracellular vesicle (EV) miRNAs are promising biomarkers for clinical diagnosis. However, their stability is a crucial concern affecting reliability and accuracy. Factors such as sample collection, processing, storage conditions, and experimental procedures impact EV miRNA stability. Studying EV miRNA stability aims to find optimal handling and storage methods, ensuring integrity and functionality throughout research. In this study, we used RT-qPCR and GlyExo-Capture technology, which can specifically capture glycosylated EVs by lectin, to assess the stability of glycosylated EV miRNAs. We found that slow acceleration centrifugation and two-step centrifugation methods were suitable for subsequent experiments. To ensure uniformity, we recommend using the two-step centrifugation method. We also studied blood storage before serum separation and recommend separation within 2 h at 4 °C or 25 °C. For separated serum samples, higher temperatures accelerated miRNA degradation, and the storage duration should be adjusted based on laboratory conditions. Short-term storage at -20 °C is acceptable for up to 3 months while avoiding repeated freeze-thaw cycles. We developed protective agents to extend the storage time at 25 °C, meeting clinical requirements. Additionally, Lakebio's cfRNA storage tubes effectively preserved the stability of miRNAs in plasma glycosylated EVs. Understanding EV miRNA stability provides insights into optimizing sample handling, storage strategies, and enhancing reliability in clinical applications.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
EVs cannot be stored for a long time since they lose their structure and bioactivity due to destruction of the lipid layers.
“However, we cannot store the EVs for a long time since they lose their structure and bioactivity due to the destruction of the lipid layers [327–329].”
From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [328]
The Discussion (page not provided) recommends freezing EVs at −80 °C or below for long-term storage spanning days to months, contrary to the claim that they cannot be stored for a long time. It says repeated freeze–thaw cycles may damage EV membranes, but does not present lipid-layer destruction and loss of bioactivity as inevitable consequences of storage. (LLM, conf=0.86)
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles
Colombo, M., Raposo, G., & Théry, C. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101512-122326 · Cited by 6,186 · see citing works
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766 · see citing works
Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation
O'Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018) · Frontiers in Endocrinology · doi:10.3389/fendo.2018.00402 · Cited by 5,287 · see citing works
The MicroRNA Spectrum in 12 Body Fluids
Weber, J. A., Baxter, D. H., Zhang, S., Huang, D. Y. C., Huang, K., Lee, M., Galas, D. J., & Wang, K. (2010) · Clinical Chemistry · doi:10.1373/clinchem.2010.147405 · Cited by 2,754 · see citing works
Witwer, K. W., Buzás, E. I., Bemis, L., Bora, A., Lässer, C., Lötvall, J. O., Hoen, E. N. M. N., Piper, M. G., Sivaraman, S., Skog, J., et al. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.20360 · Cited by 2,424 · see citing works
Exosomes: biogenesis, biologic function and clinical potential
Zhang, Y., Liu, Y., Liu, H., & Tang, W. H. (2019) · Cell & Bioscience · doi:10.1186/s13578-019-0282-2 · Cited by 2,119 · see citing works
Exosomal-like vesicles are present in human blood plasma
Caby, M.-P., Lankar, D., Vincendeau-Scherrer, C., Raposo, G., & Bonnerot, C. (2005) · International Immunology · doi:10.1093/intimm/dxh267 · Cited by 1,348 · see citing works
Plasma microRNAs are promising novel biomarkers for early detection of colorectal cancer
Huang, Z., Huang, D., Ni, S., Peng, Z., Sheng, W., & Du, X. (2009) · International Journal of Cancer · doi:10.1002/ijc.25007 · Cited by 957 · see citing works
Malignant effusions and immunogenic tumour-derived exosomes
André, F., Schartz, N. E. C., Movassagh, M., Flament, C., Pautier, P., Morice, P., Pomel, C., Lhommé, C., Escudier, B. J., Chevalier, T. L., et al. (2002) · The Lancet · doi:10.1016/s0140-6736(02)09552-1 · Cited by 953 · see citing works
The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling
Deun, J. V., Mestdagh, P., Sormunen, R. T., Cocquyt, V., Vermaelen, K., Vandesompele, J., Bracke, M., Wever, O. D., & Hendrix, A. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24858 · Cited by 941 · see citing works
Gatti, S., Bruno, S., Deregibus, M. C., Sordi, A., Cantaluppi, V., Tetta, C., & Camussi, G. (2011) · Nephrology Dialysis Transplantation · doi:10.1093/ndt/gfr015 · Cited by 814 · see citing works
Body fluid derived exosomes as a novel template for clinical diagnostics
Keller, S., Ridinger, J., Rupp, A.-K., Janssen, J. W. G., & Altevogt, P. (2011) · Journal of Translational Medicine · doi:10.1186/1479-5876-9-86 · Cited by 752 · see citing works
Tominaga, N., Kosaka, N., Ono, M., Katsuda, T., Yoshioka, Y., Tamura, K., Lötvall, J., Nakagama, H., & Ochiya, T. (2015) · Nature Communications · doi:10.1038/ncomms7716 · Cited by 724 · see citing works
Vojtech, L. N., Woo, S., Hughes, S. M., Levy, C., Ballweber, L., Sauteraud, R., Strobl, J., Westerberg, K., Gottardo, R., Tewari, M., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku347 · Cited by 572 · see citing works
Extracellular vesicles in physiological and pathological conditions
Yuana, Y., Sturk, A., & Nieuwland, R. (2013) · Blood Reviews · doi:10.1016/j.blre.2012.12.002 · Cited by 569 · see citing works
miRNA in Plasma Exosome is Stable under Different Storage Conditions
Ge, Q., Zhou, Y., Lu, J., Bai, Y., Xie, X., & Lu, Z. (2014) · Molecules · doi:10.3390/molecules19021568 · Cited by 475 · see citing works
The tumour glyco-code as a novel immune checkpoint for immunotherapy
RodrÍguez, E., Schetters, S. T. T., & van Kooyk, Y. (2018) · Nature Reviews Immunology · doi:10.1038/nri.2018.3 · Cited by 473 · see citing works
Preservation and Storage Stability of Extracellular Vesicles for Therapeutic Applications
Jeyaram, A., & Jay, S. M. (2017) · The AAPS Journal · doi:10.1208/s12248-017-0160-y · Cited by 461 · see citing works
Lacroix, R., Judicone, C., PONCELET, P., Robert, S., ARNAUD, L., Sampol, J., & Dignat‐George, F. (2011) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2011.04610.x · Cited by 401 · see citing works
Trehalose prevents aggregation of exosomes and cryodamage
Bösch, S., de Beaurepaire, L., Allard, M., Mosser, M., Heichette, C., Chrétien, D., Jégou, D., & Bach, J. (2016) · Scientific Reports · doi:10.1038/srep36162 · Cited by 399 · see citing works
Kim, S., Lechman, E. R., Bianco, N. R., Menon, R., Keravala, A., Nash, J. A., Mi, Z., Watkins, S. C., Gambotto, A. A., & Robbins, P. D. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.10.6440 · Cited by 380 · see citing works
The impact of storage on extracellular vesicles: A systematic study
Gelibter, S., Marostica, G., Mandelli, A., Siciliani, S., Podini, P., Finardi, A., & Furlan, R. (2022) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12162 · Cited by 373 · see citing works
Block, T. M., Comunale, M. A., Lowman, M., Steel, L. F., Romano, P. R., Fimmel, C. J., Tennant, B. C., London, W. T., Evans, A. A., Blumberg, B. S., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408928102 · Cited by 373 · see citing works
Wu, Y., Deng, W., & Klinke, D. J. (2015) · The Analyst · doi:10.1039/c5an00688k · Cited by 368 · see citing works
Lacroix, R., Judicone, C., Mooberry, M., Boucékine, M., Key, N. S., & Dignat‐George, F. (2013) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.12207 · Cited by 366 · see citing works
Exosome biogenesis, bioactivities and functions as new delivery systems of natural compounds
Farooqi, A. A., Desai, N. N., Qureshi, M. Z., Librelotto, D. R. N., Gasparri, M. L., Bishayee, A., Nabavi, S. M., Curti, V., & Daglia, M. (2018) · Biotechnology Advances · doi:10.1016/j.biotechadv.2017.12.010 · Cited by 359 · see citing works
Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes
Cheng, Y., Zeng, Q., Han, Q., & Xia, W. (2019) · Protein & Cell · doi:10.1007/s13238-018-0529-4 · Cited by 336 · see citing works
The Roles of Tumor-Derived Exosomes in Cancer Pathogenesis
Yang, C., & Robbins, P. D. (2011) · Clinical and Developmental Immunology · doi:10.1155/2011/842849 · Cited by 318 · see citing works
Isolation of extracellular vesicles: Determining the correct approach (Review)
Szatanek, R., Baran, J., Siedlar, M., & Baj‐Krzyworzeka, M. (2015) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2015.2194 · Cited by 281 · see citing works
Zonneveld, M. I., Brisson, A. R., van Herwijnen, M. J. C., Tan, S., van de Lest, C. H. A., Redegeld, F. A., Garssen, J., Wauben, M. H. M., & Hoen, E. N. M. N. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24215 · Cited by 274 · see citing works
Chandler, W. L., Yeung, W. T., & Tait, J. F. (2011) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2011.04283.x · Cited by 272 · see citing works
Glycosylation of extracellular vesicles: current knowledge, tools and clinical perspectives
Williams, C., Royo, F., Aizpurua‐Olaizola, O., Pazos, R., Boons, G., Reichardt, N., & Falcón‐Pérez, J. M. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1442985 · Cited by 260 · see citing works
Methodology for isolation, identification and characterization of microvesicles in peripheral blood
Jayachandran, M., Miller, V. M., Heit, J. A., & Owen, W. G. (2011) · Journal of Immunological Methods · doi:10.1016/j.jim.2011.10.012 · Cited by 229 · see citing works
The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo
Schageman, J. J., Zeringer, E., Li, M., Barta, T. J., Lea, K., Gu, J. H., Magdaleno, S. M., Setterquist, R. A., & Vlassov, A. V. (2013) · BioMed Research International · doi:10.1155/2013/253957 · Cited by 179 · see citing works
Clayton, A., Boilard, É., Buzás, E. I., Cheng, L., Falcón‐Pérez, J. M., Gardiner, C., Gustafson, D., Gualerzi, A., Hendrix, A., Hoffman, A. M., et al. (2019) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1647027 · Cited by 143 · see citing works
Prospective Therapeutic Applications of Platelet Extracellular Vesicles
Johnson, J., Wu, Y., Blyth, C., Lichtfuss, G. F., Goubran, H. A., & Burnouf, T. P. R. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.10.004 · Cited by 143 · see citing works
DNA in serum extracellular vesicles is stable under different storage conditions
Yang, J., Chen, K., Wang, Z., Wang, Y., Liu, J., Lin, L., Shao, Y., Gao, L., Yin, H., Cui, C., et al. (2016) · BMC Cancer · doi:10.1186/s12885-016-2783-2 · Cited by 139 · see citing works
Global analyses of the dynamics of mammalian microRNA metabolism
Kingston, E. R., & Bartel, D. P. (2019) · Genome Research · doi:10.1101/gr.251421.119 · Cited by 133 · see citing works
Martins, Á. M., Ramos, C. C., Freitas, D. P., & Reis, C. A. (2021) · Cells · doi:10.3390/cells10010109 · Cited by 127 · see citing works
A comprehensive joint analysis of the long and short RNA transcriptomes of human erythrocytes
Doss, J. F., Corcoran, D. L., Jima, D. D., Telen, M. J., Davé, S. S., & Chi, J. (2015) · BMC Genomics · doi:10.1186/s12864-015-2156-2 · Cited by 117 · see citing works
The role of microRNAs in skin fibrosis
Babalola, O., Mamalis, A., Lev‐Tov, H. A., & Jagdeo, J. R. (2013) · Archives of Dermatological Research · doi:10.1007/s00403-013-1410-1 · Cited by 98 · see citing works
MiR-150 promotes cellular metastasis in non-small cell lung cancer by targeting FOXO4
Li, H., Ouyang, R., Wang, Z., Zhou, W., Chen, H., Jiang, Y., Zhang, Y., Li, H., Liao, M., Wang, W., et al. (2016) · Scientific Reports · doi:10.1038/srep39001 · Cited by 92 · see citing works
Differential profiling of human red blood cells during storage for 52 selected microRNAs
Kannan, M., & Atreya, C. D. (2010) · Transfusion · doi:10.1111/j.1537-2995.2010.02585.x · Cited by 79 · see citing works
Wong, K. H. K., Tessier, S. N., Miyamoto, D. T., Miller, K. L., Bookstaver, L. D., Carey, T. R., Stannard, C. J., Thapar, V., Tai, E. C., Vo, K. D., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-01705-y · Cited by 66 · see citing works
Tegegn, T. Z., Paoli, S. H. D., Orečná, M., Elhelu, O. K., Woodle, S. A., Tarandovskiy, I. D., Ovanesov, M. V., & Šimák, J. (2016) · Journal of Extracellular Vesicles · doi:10.3402/jev.v5.30422 · Cited by 63 · see citing works
Centrifugation affects the purity of liquid biopsy‐based tumor biomarkers
Rikkert, L. G., van der Pol, E., van Leeuwen, T. G. J. M., Nieuwland, R., & Coumans, F. A. W. (2018) · Cytometry Part A · doi:10.1002/cyto.a.23641 · Cited by 51 · see citing works
Cloos, A., Ghodsi, M., Stommen, A., Vanderroost, J., Dauguet, N., Pollet, H., D’Auria, L., Mignolet, É., Larondelle, Y., Terrasi, R., et al. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.00712 · Cited by 46 · see citing works
The Role of Physical Stabilization in Whole Blood Preservation
Wong, K. H. K., Sandlin, R. D., Carey, T. R., Miller, K. L., Shank, A. T., Öklü, R., Maheswaran, S., Haber, D. A., Irimia, D., Stott, S. L., et al. (2016) · Scientific Reports · doi:10.1038/srep21023 · Cited by 46 · see citing works
Buntsma, N. C., Gąsecka, A., Roos, Y. B. W. E. M., van Leeuwen, T. G. J. M., van der Pol, E., & Nieuwland, R. (2021) · Platelets · doi:10.1080/09537104.2021.1991569 · Cited by 42 · see citing works
Novel Approach to Fecal Occult Blood Testing by Assay of Erythrocyte-Specific microRNA Markers
Wu, C. W., Cao, X., Berger, C. K., Foote, P. H., Mahoney, D. W., Simonson, J. A., Anderson, B. W., Yab, T. C., Taylor, W. R., Boardman, L. A., et al. (2017) · Digestive Diseases and Sciences · doi:10.1007/s10620-017-4627-6 · Cited by 37 · see citing works
Effects of Preanalytic Variables on Circulating MicroRNAs in Whole Blood
Zhao, H., Shen, J., Hu, Q., Davis, W., Medico, L., Wang, D., Yan, L., Guo, Y., Liu, B., Qin, M., et al. (2014) · Cancer Epidemiology Biomarkers & Prevention · doi:10.1158/1055-9965.epi-14-0550 · Cited by 36 · see citing works
Sarachana, T., Kulkarni, S., & Atreya, C. D. (2015) · Transfusion · doi:10.1111/trf.13235 · Cited by 30 · see citing works
Kim, H. J., Rames, M. J., Yunga, S. T., Armstrong, R. J., Morita, M., Ngo, A. T. P., McCarty, O. J. T., Çivitçi, F., Morgan, T. K., & Ngo, T. T. M. (2022) · Scientific Reports · doi:10.1038/s41598-022-06088-9 · Cited by 21 · see citing works
Exosomal protein interactors as emerging therapeutic targets in urothelial bladder cancer
Kumari, N., Saxena, S., & Agrawal, U. (2015) · Journal of the Egyptian National Cancer Institute · doi:10.1016/j.jnci.2015.02.002 · Cited by 19 · see citing works
Expression of potassium channels in epithelial cells depends on calcium-activated cell-cell contacts
Talavera‐Adame, D., Ponce, A., Fiorentino, R., Gonz�lez-Mariscal, L., Contreras, R. G., S�nchez, S., Garcı́a-Villegas, R., Vald�s, J., & Cereijido, M. (1995) · The Journal of Membrane Biology · doi:10.1007/bf00233450 · Cited by 17 · see citing works
Degradation of serum microRNAs during transient storage of serum samples at 4℃
Aiso, T., Takigami, S., Yamaki, A., & Ohnishi, H. (2017) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/0004563217704233 · Cited by 16 · see citing works
Faraldi, M., Gomarasca, M., Perego, S., Sansoni, V., Banfi, G., & Lombardi, G. (2020) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2020-1296 · Cited by 12 · see citing works
Gavioli, G., Razzoli, A., Bedolla, D. E., Bartolomeo, E. D., Quartieri, E., Iotti, B., Berni, P., Birarda, G., Vaccari, L., Schiroli, D., et al. (2023) · Platelets · doi:10.1080/09537104.2023.2281943 · Cited by 4 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Different storage and freezing protocols for extracellular vesicles: a systematic review
Ahmadian, S., Jafari, N., Tamadon, A., Ghaffarzadeh, A., Rahbarghazi, R., & Mahdipour, M. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-04005-7 · Cited by 197 · see citing works
Ljungström, M., & Oltra, E. (2025) · Genes · doi:10.3390/genes16030330 · Cited by 48 · see citing works
Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions
Choi, H. K., Chen, M., Goldston, L. L., & Lee, K.-B. (2024) · Nano Convergence · doi:10.1186/s40580-024-00426-5 · Cited by 26 · see citing works
Guo, S., Wang, X., Shan, D., Xiao, Y., Ju, L., Zhang, Y., Wang, G., & Qian, K. (2024) · Biomarker Research · doi:10.1186/s40364-024-00661-2 · Cited by 21 · see citing works
Barathan, M., Ng, S. L., Lokanathan, Y., Ng, M. H., & Law, J. X. (2024) · Pharmaceutics · doi:10.3390/pharmaceutics16050654 · Cited by 20 · see citing works
Exosomes in Diabetic Wound Healing: Mechanisms, Applications, and Perspectives
Yi, J., Tang, Q., Sun, S., Xie, H., Wang, L., & Yin, X. (2025) · Diabetes Metabolic Syndrome and Obesity · doi:10.2147/dmso.s532885 · Cited by 16 · see citing works
MARATOVICH, B. A., Al-Yozbaki, M., Ashirbekov, Y., Satken, K., Abaildayev, A., Yeleussizov, A., Jainakbayev, N. T., Sharipov, K. O., & Wilson, C. M. (2025) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2025.1676530 · Cited by 12 · see citing works
Challenges of using microRNAs as potential disease biomarkers. A review
Zinovkin, R. A., & Sakharov, I. Y. (2024) · Microchemical Journal · doi:10.1016/j.microc.2024.111379 · Cited by 7 · see citing works
Cook‐Calvete, A., Delgado-Marín, M., Fernandez-Rodriguez, B. M., Zaragoza, C., & Saura, M. (2025) · Biomolecules · doi:10.3390/biom15111548 · Cited by 6 · see citing works
Huang, H., Xu, W., Hao, X., Sun, P., Guo, M., Li, M., Liu, X., Peng, Y., Han, R., Tang, T., et al. (2026) · Journal of Controlled Release · doi:10.1016/j.jconrel.2026.114706 · Cited by 6 · see citing works
Hu, Y., Sun, Z., Zeng, W., Qiu, Y., Xu, Z., Zhang, J., & Feng, G. (2025) · Molecular Diversity · doi:10.1007/s11030-024-11098-y · Cited by 6 · see citing works
Clinical Significance of MicroRNA-299-3p in Coronary Artery Disease Based on Bioinformatics Analysis
Wu, J., Wu, S., Liu, D., & Chen, L. (2024) · Cell Biochemistry and Biophysics · doi:10.1007/s12013-024-01431-5 · Cited by 4 · see citing works
Quantification of EV-associated miRNA in liquid biopsies for biomarker signature development
Yu, M. S. C., Grätz, C., Chiang, D. M., Loudig, O. D., Zheng, Y., Hladik, C., Hannafon, B. N., Dogra, N., Kirchner, B., & Pfaffl, M. W. (2026) · Extracellular Vesicles and Circulating Nucleic Acids · doi:10.20517/evcna.2026.19 · Cited by 2 · see citing works
Role of N -Glycosylation in Gastrointestinal Cancers
Xu, R., Balmer, L. A., Chen, G., & Song, M. (2024) · OMICS A Journal of Integrative Biology · doi:10.1089/omi.2024.0174 · Cited by 1 · see citing works
MicroRNAs as Potential Biomarkers for Alzheimer’s Disease in Women
Huang, S., Zhong, L., Zheng, L. S., & Shi, J. (2025) · Genes · doi:10.3390/genes16080943
He, J., Wang, L., Yu, B., Huang, W., Ouyang, C., Zhou, M., Hu, R., & Chen, Z. (2025) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2025.1694257
Wang, N., Hu, M., Zhao, W., Zhang, Y., Wu, H., Wang, J., & Yang, C. (2025) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2025.117190
MicroRNAs in oral squamous cell carcinoma progression
Edwin, M. A., Salaman, S. D., Gokul, S., & Ammapalli, G. (2026) · Cell Death · doi:10.1016/j.celld.2026.100013
Li, A., Yuan, Z., Zhai, J., Zhang, X., Zhang, T., Duan, S., & Yu, W. (2026) · Journal of Dairy Science · doi:10.3168/jds.2026-28275
Sun, X.-Y., Fu, N., Zhao, D.-D., Wang, Z.-L., Cao, L., Qiu, J.-L., Li, Y., Gao, W., Duan, J.-L., Li, L., et al. (2026) · World Journal of Gastrointestinal Oncology · doi:10.4251/wjgo.118948
Exosome-Based Drug Delivery Systems in Colorectal Cancer: Current Evidence and Future Perspectives
da Silva, L. H. D., Floriano, J. F., & Chorilli, M. (2026) · The AAPS Journal · doi:10.1208/s12248-026-01301-3
Systematic evaluation of microRNA stability during long term storage of urine
Satomura, A., Ando, Y., Mikami, M., & Ichikawa, Y. (2026) · Discover Applied Sciences · doi:10.1007/s42452-026-09526-9
Traditional methods for microRNA extraction
Gupta, P. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-45118-8.00004-7
- Authors
- Ma, C., Ding, R., Hao, K., Du, W., Xu, L., Gao, Q., & Yu, C.
- Affiliations
- Beijing University of Chemical Technology (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Beijing Hotgen Biotech Co., Ltd., Beijing 102600, China
- Corresponding author
- Gao, Qi
- Yu, Changyuan
- ORCID
- Gao, Qi
- Venue
- Molecules
- Publisher
- MDPI AG
- Published
- 2023-12-23
- Volume
- 29
- Issue
- 1
- Pages
- 103
- ISSN
- 1420-3049 (ISSN-L)
- PMC
- PMC10780163
- OpenAlex
- W4390175377
- Cited by
- 22
- References
- 58
- Citations by year
- 2026: 7 · 2025: 8 · 2024: 7
- FWCI
- 1.96
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Molecules (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (82174531, XK2023-13, 2022AB022)
- National High-Level Hospital Clinical Research Funding (82174531, XK2023-13, 2022AB022)
- Scientific and Technological Research Project of the Xinjiang Production and Construction Corps (82174531, XK2023-13, 2022AB022)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- extracellular vesicles, miRNA stability, blood sample storage, centrifugation, serum separation, storage temperature, freeze-thaw cycles, biomarkers
- MeSH terms
- Acceleration, Extracellular Vesicles, Glycosylation, Reproducibility of Results, MicroRNAs
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
