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Cited by 16Celiac Disease Research and ManagementEosinophilic EsophagitisWheat and Barley Genetics and Pathology

Intraepithelial Lymphocyte Cytometric Pattern Is a Useful Diagnostic Tool for Coeliac Disease Diagnosis Irrespective of Degree of Mucosal Damage and Age—A Validation Cohort

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Ruiz-Ramírez, Pablo, Carreras, Gerard, Fajardo, Ingrid, Tristán, Eva, Carrasco, Anna, Salvador, Isabel, Zabana, Yamile, Andújar, Xavier +6 more·2021·Nutrients
DOI 10.3390/nu13051684PMC PMC8155844
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Abstract

Introduction: The study of intraepithelial lymphocytes (IEL) by flow cytometry is a useful tool in the diagnosis of coeliac disease (CD). Previous data showed that an increase in %TCRγδ+ and decrease of %CD3− IEL constitute a typical CD cytometric pattern with a specificity of 100%. However, there are no data regarding whether there are differences in the %TCRγδ+ related to sex, age, titers of serology, and degree of histological lesion. Study aims: To confirm the high diagnostic accuracy of the coeliac cytometric patterns. To determine if there are differences between sex, age, serology titers, and histological lesion grade. Results: We selected all patients who fulfilled “4 of 5” rule for CD diagnosis (n = 169). There were no differences in %TCRγδ+ between sexes (p = 0.909), age groups (p = 0.986), serology titers (p = 0.53) and histological lesion grades (p = 0.41). The diagnostic accuracy of complete CD cytometric pattern was: specificity 100%, sensitivity 82%, PPV 100%, NPV 47%. Conclusion: We confirmed, in a validation cohort, the high diagnostic accuracy of complete CD pattern irrespective of sex, age, serology titers, and grade of mucosal lesion.

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  • The diagnostic efficacy of iIEL assessment by flow cytometry has been confirmed in the diagnosis and follow-up of the different forms of Refractory celiac disease.

    “and in the diagnosis and follow-up of the different forms of Refractory celiac disease [310, 319].”

    From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [319]

    The Introduction (page unavailable) says IEL flow cytometry has been shown to be valuable for diagnosing refractory coeliac disease. It does not address follow-up or distinguish different forms of refractory disease; the diagnostic accuracy reported in the Results concerns the broader coeliac disease cohort, not refractory disease specifically. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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Authors
Ruiz-Ramírez, P., Carreras, G., Fajardo, I., Tristán, E., Carrasco, A., Salvador, I., Zabana, Y., Andújar, X., Ferrer, C., Horta, D., Loras, C., García-Puig, R., Fernández-Bañares, F., & Esteve, M.
Affiliations
  • University Hospital Mútua de Terrassa (ES)
  • Instituto de Salud Carlos III (ES)
  • Department of Gastroenterology, Hospital Universitari Mútua Terrassa, Universitat de Barcelona, 08221 Barcelona, Spain
ORCID
Venue
Nutrients
Publisher
MDPI AG
Published
2021-05-15
Volume
13
Issue
5
Pages
1684
ISSN
2072-6643 (ISSN-L)
DOI
10.3390/nu13051684
PMC
PMC8155844
OpenAlex
W3161825070
Cited by
16
References
29
Citations by year
2026: 2 · 2025: 2 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 4
FWCI
2.74
Type
journal article
Language
en
Open access
Licence
Locations
  • Nutrients (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Gastroenterology › Celiac Disease Research and Management
  • Health Sciences › Medicine › Surgery › Eosinophilic Esophagitis
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
Keywords
celiac disease, intraepithelial lymphocytes, diagnostic accuracy, mucosal injury, sex differences, age differences
MeSH terms
Intraepithelial Lymphocytes, Adolescent, Adult, Aged, Aged, 80 and over, Biopsy, Celiac Disease, Child, Child, Preschool, Female, Flow Cytometry, Humans, Immunoglobulin A, Male, Middle Aged, Cohort Studies, Receptors, Antigen, T-Cell, gamma-delta, Young Adult

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