Inhibition of mixed biofilms of Candida albicans and methicillin-resistant Staphylococcus aureus by positively charged silver nanoparticles and functionalized silicone elastomers
Open accessAbstract
Both bacterial and fungal organisms display the ability to form biofilms; however, mixed bacterial/fungal biofilms are particularly difficult to control and eradicate. The opportunistic microbial pathogens Candida albicans and Staphylococcus aureus are among the most frequent causative agents of healthcare-acquired infections, and are often co-isolated forming mixed biofilms, especially from contaminated catheters. These mixed species biofilms display a high level of antibiotic resistance; thus, these infections are challenging to treat resulting in excess morbidity and mortality. In the absence of effective conventional antibiotic treatments, nanotechnology-based approaches represent a promising alternative for the treatment of highly recalcitrant polymicrobial biofilm infections. Our group has previously reported on the activity of pure positively charged silver nanoparticles synthesized by a novel microwave technique against single-species biofilms of C. albicans and S. aureus. Here, we have expanded our observations to demonstrate that that silver nanoparticles display dose-dependent activity against dual-species C. albicans/S. aureus biofilms. Moreover, the same nanoparticles were used to functionalize catheter materials, leading to the effective inhibition of the mixed fungal/bacterial biofilms. Overall, our results indicate the potent activity of silver nanoparticles against these cross-kingdom biofilms. More studies are warranted to examine the ability of functionalized catheters in the prevention of catheter-related bloodstream infections.
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Nanoparticles display potent activity against mixed Candida/bacterial biofilms, which normally exhibit high levels of resistance against both antibacterial and antifungal antibiotics.
“Importantly, nanoparticles have been described to display potent activity against mixed Candida/bacterial biofilms which normally exhibit high levels of resistance against both antibacterial and antifungal antibiotics [86].”
From Antifungal therapy of Candida biofilms: Past, present and future (2023) · cites this paper as [86]
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Cites
The bactericidal effect of silver nanoparticles
Morones‐Ramírez, J. R., Elechiguerra, J. L., Camacho, A., Holt, K. B., Kouri, J. B., Tapia-Ramı́rez, J., & Yacamán, M. J. (2005) · Nanotechnology · doi:10.1088/0957-4484/16/10/059 · Cited by 6,690 · see citing works
Targeting microbial biofilms: current and prospective therapeutic strategies
Koo, H., Allan, R. N., Howlin, R. P., Stoodley, P., & Hall‐Stoodley, L. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2017.99 · Cited by 1,833 · see citing works
Interaction of silver nanoparticles with HIV-1
Elechiguerra, J. L., Burt, J. L., Morones‐Ramírez, J. R., Camacho-Bragado, A., Gao, X., Lara, H. H., & Yacamán, M. J. (2005) · Journal of Nanobiotechnology · doi:10.1186/1477-3155-3-6 · Cited by 1,713 · see citing works
Self-citationHealth care-associated infections – an overview
Haque, M., Sartelli, M., McKimm, J., & Bakar, M. A. (2018) · Infection and Drug Resistance · doi:10.2147/idr.s177247 · Cited by 1,207 · see citing works
Zaoutis, T. E., Argon, J., Chu, J., Berlin, J. A., Walsh, T. J., & Feudtner, C. (2005) · Clinical Infectious Diseases · doi:10.1086/496922 · Cited by 784 · see citing works
Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds
Lara, H. H., Garza-Treviño, E. N., Ixtepan-Turrent, L., et al. (2011) · Journal of Nanobiotechnology · doi:10.1186/1477-3155-9-30 · Cited by 743 · see citing works
Self-citationEnergy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis
Scimeca, M., Bischetti, S., Lamsira, H. K., et al. (2018) · European Journal of Histochemistry · doi:10.4081/ejh.2018.2841 · Cited by 527 · see citing works
Harriott, M. M., & Noverr, M. C. (2009) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00657-09 · Cited by 526 · see citing works
Prevention and control of biofilm-based medical-device-related infections
Francolini, I., & Donelli, G. (2010) · FEMS Immunology & Medical Microbiology · doi:10.1111/j.1574-695x.2010.00665.x · Cited by 473 · see citing works
A review of the recent advances in antimicrobial coatings for urinary catheters
Singha, P., Locklin, J., & Handa, H. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.11.070 · Cited by 454 · see citing works
Effect of silver nanoparticles on Candida albicans biofilms: an ultrastructural study
Lara, H. H., Romero-Urbina, D. G., Pierce, C., Lopez-Ribot, J. L., Arellano-Jiménez, M. J., & Jose-Yacaman, M. (2015) · Journal of Nanobiotechnology · doi:10.1186/s12951-015-0147-8 · Cited by 323 · see citing works
Self-citationImportance of Candida–bacterial polymicrobial biofilms in disease
Harriott, M. M., & Noverr, M. C. (2011) · Trends in Microbiology · doi:10.1016/j.tim.2011.07.004 · Cited by 315 · see citing works
Kong, E. F., Tsui, C., Kucharíková, S., Andes, D. R., Dijck, P. V., & Jabra‐Rizk, M. A. (2016) · mBio · doi:10.1128/mbio.01365-16 · Cited by 298 · see citing works
Peters, B. M., Jabra‐Rizk, M. A., Scheper, M. A., Leid, J. G., Costerton, J. W., & Shirtliff, M. E. (2010) · FEMS Immunology & Medical Microbiology · doi:10.1111/j.1574-695x.2010.00710.x · Cited by 288 · see citing works
Ramasamy, M., & Lee, J. (2016) · BioMed Research International · doi:10.1155/2016/1851242 · Cited by 285 · see citing works
Schlecht, L. M., Peters, B. M., Krom, B. P., Freiberg, J. A., Hänsch, G. M., Filler, S. G., Jabra‐Rizk, M. A., & Shirtliff, M. E. (2014) · Microbiology · doi:10.1099/mic.0.083485-0 · Cited by 282 · see citing works
Prevention and treatment of Staphylococcus aureus biofilms
Bhattacharya, M., Wozniak, D. J., Stoodley, P., & Hall‐Stoodley, L. (2015) · Expert Review of Anti-infective Therapy · doi:10.1586/14787210.2015.1100533 · Cited by 271 · see citing works
Uppuluri, P., Nett, J., Heitman, J., & Andes, D. (2008) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01397-07 · Cited by 228 · see citing works
Kean, R., Rajendran, R., Haggarty, J. H., Townsend, E. R., Short, B., Burgess, K. E., Lang, S., Millington, O. R., Mackay, W. G., Williams, C., et al. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00258 · Cited by 206 · see citing works
Current Antimycotics, New Prospects, and Future Approaches to Antifungal Therapy
Wall, G., & Lopez-Ribot, J. L. (2020) · Antibiotics · doi:10.3390/antibiotics9080445 · Cited by 176 · see citing works
Self-citationThe Candida albicans Biofilm Matrix: Composition, Structure and Function
Pierce, C. G., Vila, T. V. M., Romo, J. A., Montelongo‐Jauregui, D., Wall, G., Ramasubramanian, A. K., & Lopez-Ribot, J. L. (2017) · Journal of Fungi · doi:10.3390/jof3010014 · Cited by 160 · see citing works
Self-citationThe Interface between Fungal Biofilms and Innate Immunity
Kernien, J. F., Snarr, B. D., Sheppard, D. C., & Nett, J. E. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2017.01968 · Cited by 151 · see citing works
Antimicrobial strategies for urinary catheters
Zhu, Z., Wang, Z., Li, S., & Yuan, X. F. (2018) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36561 · Cited by 149 · see citing works
Candida spp./Bacteria Mixed Biofilms
Rodrigues, M. E., Gomes, F., & Rodrigues, C. F. (2019) · Journal of Fungi · doi:10.3390/jof6010005 · Cited by 149 · see citing works
Antimicrobial Treatment of Polymeric Medical Devices by Silver Nanomaterials and Related Technology
Polívková, M., Hubáček, T., Staszek, M., Švorčı́k, V., & Siegel, J. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18020419 · Cited by 129 · see citing works
Ma, S., Moser, D., Han, F., et al. (2020) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2020.116254 · Cited by 127 · see citing works
Pierce, C. G., Chaturvedi, A. K., Lazzell, A. L., Powell, A. T., Saville, S. P., McHardy, S. F., & Lopez-Ribot, J. L. (2015) · npj Biofilms and Microbiomes · doi:10.1038/npjbiofilms.2015.12 · Cited by 124 · see citing works
Self-citationLara, H. H., Ixtepan-Turrent, L., Jose Yacaman, M., & Lopez-Ribot, J. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.9b20708 · Cited by 116 · see citing works
Self-citationLara, H. H., Guisbiers, G., Mendoza, J., Mimun, L. C., Vincent, B., Lopez-Ribot, J. L., & Nash, K. L. (2018) · International Journal of Nanomedicine · doi:10.2147/ijn.s151285 · Cited by 104 · see citing works
Self-citationHealthcare‐acquired infections: prevention strategies
Fernando, S. A., Gray, T. J., & Gottlieb, T. (2017) · Internal Medicine Journal · doi:10.1111/imj.13642 · Cited by 98 · see citing works
Akers, K. S., Mende, K., Cheatle, K. A., et al. (2014) · BMC Infectious Diseases · doi:10.1186/1471-2334-14-190 · Cited by 97 · see citing works
Optical and Digital Microscopic Imaging Techniques and Applications in Pathology
Chen, X., Zheng, B., & Liu, H. (2011) · Analytical Cellular Pathology · doi:10.1155/2011/150563 · Cited by 91 · see citing works
Wall, G., Chaturvedi, A. K., Wormley, F. L., Wiederhold, N. P., Patterson, H. P., Patterson, T. F., & Lopez-Ribot, J. L. (2018) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01084-18 · Cited by 89 · see citing works
Self-citationWang, H., Tong, H., Liu, H., Wang, Y., Wang, R., Gao, H., Yu, P., Lv, Y., Chen, S., Wang, G., et al. (2018) · Annals of Intensive Care · doi:10.1186/s13613-018-0416-4 · Cited by 86 · see citing works
Todd, O. A., & Peters, B. M. (2019) · Journal of Fungi · doi:10.3390/jof5030081 · Cited by 83 · see citing works
Montelongo‐Jauregui, D., Srinivasan, A., Ramasubramanian, A. K., & Lopez-Ribot, J. L. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00686 · Cited by 77 · see citing works
Self-citationRomero-Urbina, D., Lara, H. H., Velázquez‐Salazar, J. J., Arellano-Jiménez, M. J., Larios, E., Srinivasan, A., Lopez-Ribot, J. L., & Yacamán, M. J. (2015) · Beilstein Journal of Nanotechnology · doi:10.3762/bjnano.6.246 · Cited by 68 · see citing works
Self-citationCatheter removal and outcomes of multidrug-resistant central-line-associated bloodstream infection
Burnham, J. P., Rojek, R. P., & Kollef, M. H. (2018) · Medicine · doi:10.1097/md.0000000000012782 · Cited by 67 · see citing works
Prevention of Device-Related Healthcare-Associated Infections
Septimus, E. J., & Moody, J. A. (2016) · F1000Research · doi:10.12688/f1000research.7493.1 · Cited by 56 · see citing works
Optical and digital microscopic imaging techniques and applications in pathology.
Chen, X., Zheng, B., & Liu, H. (2011) · PubMed · doi:10.3233/acp-2011-0006 · Cited by 55 · see citing works
Vila, T. V. M., Kong, E. F., Ibrahim, A., Piepenbrink, K. H., Shetty, A. C., McCracken, C. L., Bruno, V. M., & Jabra‐Rizk, M. A. (2019) · Virulence · doi:10.1080/21505594.2019.1635418 · Cited by 50 · see citing works
Efficacy of silver nanoparticles against the adults and eggs of monogenean parasites of fish
Pimentel-Acosta, C. A., Morales-Serna, F. N., Chávez‐Sánchez, M. C., et al. (2019) · Parasitology Research · doi:10.1007/s00436-019-06315-9 · Cited by 44 · see citing works
From Biology to Drug Development: New Approaches to Combat the Threat of Fungal Biofilms
Pierce, C. G., Srinivasan, A., Ramasubramanian, A. K., & Lopez-Ribot, J. L. (2015) · Microbiology Spectrum · doi:10.1128/microbiolspec.mb-0007-2014 · Cited by 35 · see citing works
Self-citationFuentes-Valencia, M. A., Fajer-Avila, E. J., Chávez‐Sánchez, M. C., et al. (2019) · Experimental Parasitology · doi:10.1016/j.exppara.2019.107825 · Cited by 14 · see citing works
Comparison between digital and optical microscopy: Analysis in a mouse gut inflammation model
Silva, A. P. E., Campos, S. M. N., Guimarães, I. M., et al. (2017) · Biomedical Reports · doi:10.3892/br.2017.950 · Cited by 8 · see citing works
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Antifungal therapy of Candida biofilms: Past, present and future
Ajetunmobi, O. H., Badali, H., Romo, J. A., Ramage, G., & Lopez-Ribot, J. L. (2023) · Biofilm · doi:10.1016/j.bioflm.2023.100126 · Cited by 52 · see citing works
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- Authors
- Lara, H. H., & Lopez-Ribot, J. L.
- Affiliations
- The University of Texas at San Antonio (US)
- Corresponding author
- Lara, Humberto H.
- Lopez-Ribot, Jose L.
- Venue
- Pathogens
- Publisher
- MDPI AG
- Published
- 2020-09-25
- Volume
- 9
- Issue
- 10
- Pages
- 784
- ISSN
- 2076-0817 (ISSN-L)
- PMC
- PMC7600790
- OpenAlex
- W3087852848
- Cited by
- 36
- References
- 45
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 10 · 2023: 5 · 2022: 6 · 2021: 7
- FWCI
- 1.46
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Pathogens (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Margaret Batts Tobin Foundation (no number)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Keywords
- Candida albicans, methicillin-resistant Staphylococcus aureus, polymicrobial biofilms, silver nanoparticles, positively charged nanoparticles, silicone rubber, catheter-related bloodstream infection, nosocomial infections, antibiotic resistance, microwave-assisted synthesis
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