Synergistic antifungal effect of chitosan-stabilized selenium nanoparticles synthesized by pulsed laser ablation in liquids against Candida albicans biofilms
Open accessAbstract
Background: Candida albicans is a major opportunistic fungal pathogen. One of the most important virulence factors that contribute to the pathogenesis of candidiasis is its ability to form biofilms. A key characteristic of Candida biofilms is their resistance to antifungal agents. Due to significant morbidity and mortality rates related to biofilm-associated drug resistance, there is an urgency to develop novel nanotechnology-based approaches preventing biofilm-related infections. Methods: In this study, we report, for the first time, the synthesis of selenium nanoparticles by irradiating selenium pellets by nanosecond pulsed laser ablation in liquid chitosan as a capping agent. Synergy of the fungicidal effect of selenium nanoparticles and chitosan was quantified by the combination index theorem of Chou–Talalay. Results: This drug combination resulted in a potent fungicidal effect against a preformed C. albicans biofilm in a dose–response manner. By advanced electron microscopy techniques, we documented the adhesive and permeabilizing properties of chitosan, therefore allowing selenium nanoparticles to enter as the cell wall of the yeast became disrupted and distorted. Most importantly, we demonstrated a potent quantitative synergistic effect when compounds such as selenium and chitosan are combined. Conclusion: These chitosan-stabilized selenium nanoparticles could be used for ex vivo applications such as sterilizers for surfaces and biomedical devices. Keywords: selenium nanoparticles, laser ablation in liquids, Candida albicans , biofilm, chitosan, synergy
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Other types of nanoparticles, including polymeric nanoparticles (i.e. chitosan, curcumin), display inhibitory activity against Candida biofilms.
“Other types of nanoparticles, including different polymeric nanoparticles (i.e. chitosan, curcumin) also display inhibitory activity against Candida biofilms [84,85].”
From Antifungal therapy of Candida biofilms: Past, present and future (2023) · cites this paper as [85]
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- Authors
- Lara, H. H., Guisbiers, G., Mendoza, J., Mimun, L. C., Vincent, B., Lopez-Ribot, J. L., & Nash, K. L.
- Affiliations
- The University of Texas at San Antonio (US)
- United States Army (US)
- Corresponding author
- Lara, Humberto H
- Venue
- International Journal of Nanomedicine
- Publisher
- Informa UK Limited
- Published
- 2018-05
- Volume
- Volume 13
- Pages
- 2697-2708
- ISSN
- 1176-9114 (ISSN-L)
- 1178-2013
- PMC
- PMC5937483
- OpenAlex
- W2800887222
- Cited by
- 104
- References
- 62
- Citations by year
- 2026: 11 · 2025: 7 · 2024: 23 · 2023: 14 · 2022: 16 · 2021: 14 · 2020: 12 · 2019: 6 · 2018: 1
- FWCI
- 3.30
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Nanomedicine (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
- Funders
- National Science Foundation (0934218)
- Welch Foundation (AX-1615, -1615)
- San Antonio Area Foundation (G12MD007591)
- National Institutes of Health (AX-1615)
- National Institute of General Medical Sciences (R25GM060655)
- Division of Materials Research (DMR0934218, 0934218)
- National Institute on Minority Health and Health Disparities (G12MD007591)
- Air Force Office of Scientific Research (FA9550-, FA9550-15-1-0109)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Laser-Ablation Synthesis of Nanoparticles
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Keywords
- selenium nanoparticles, chitosan, Candida albicans, fungal biofilms, antifungal synergy, pulsed laser ablation in liquid, combination index, candidiasis, nanoparticle capping agents, surface sterilization
- MeSH terms
- Antifungal Agents, Candida albicans, Cell Line, Dose-Response Relationship, Drug, Drug Therapy, Combination, Humans, Lasers, Microbial Sensitivity Tests, Selenium, Biofilms, Nanotechnology, Chitosan, Nanoparticles
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