Cited by 104Laser-Ablation Synthesis of NanoparticlesAntifungal resistance and susceptibilityNanoparticles: synthesis and applications

Synergistic antifungal effect of chitosan-stabilized selenium nanoparticles synthesized by pulsed laser ablation in liquids against Candida albicans biofilms

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Lara, Humberto H, Guisbiers, Gregory, Mendoza, Jonathan, Mimun, Lawrence C, Vincent, Brandy, Lopez-Ribot, Jose L, Nash, Kelly L·2018·International Journal of Nanomedicine

Abstract

Background: Candida albicans is a major opportunistic fungal pathogen. One of the most important virulence factors that contribute to the pathogenesis of candidiasis is its ability to form biofilms. A key characteristic of Candida biofilms is their resistance to antifungal agents. Due to significant morbidity and mortality rates related to biofilm-associated drug resistance, there is an urgency to develop novel nanotechnology-based approaches preventing biofilm-related infections. Methods: In this study, we report, for the first time, the synthesis of selenium nanoparticles by irradiating selenium pellets by nanosecond pulsed laser ablation in liquid chitosan as a capping agent. Synergy of the fungicidal effect of selenium nanoparticles and chitosan was quantified by the combination index theorem of Chou–Talalay. Results: This drug combination resulted in a potent fungicidal effect against a preformed C. albicans biofilm in a dose–response manner. By advanced electron microscopy techniques, we documented the adhesive and permeabilizing properties of chitosan, therefore allowing selenium nanoparticles to enter as the cell wall of the yeast became disrupted and distorted. Most importantly, we demonstrated a potent quantitative synergistic effect when compounds such as selenium and chitosan are combined. Conclusion: These chitosan-stabilized selenium nanoparticles could be used for ex vivo applications such as sterilizers for surfaces and biomedical devices. Keywords: selenium nanoparticles, laser ablation in liquids, Candida albicans , biofilm, chitosan, synergy

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  • Other types of nanoparticles, including polymeric nanoparticles (i.e. chitosan, curcumin), display inhibitory activity against Candida biofilms.

    “Other types of nanoparticles, including different polymeric nanoparticles (i.e. chitosan, curcumin) also display inhibitory activity against Candida biofilms [84,85].”

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  1. [46]Bijnsdorp IV Giovannetti E Peters GJ Analysis of drug interactions Methods Mol Biol 2011 731 421 434 21516426

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Authors
Lara, H. H., Guisbiers, G., Mendoza, J., Mimun, L. C., Vincent, B., Lopez-Ribot, J. L., & Nash, K. L.
Affiliations
  • The University of Texas at San Antonio (US)
  • United States Army (US)
Corresponding author
Lara, Humberto H
Venue
International Journal of Nanomedicine
Published
2018-05
Volume
Volume 13
Pages
2697-2708
ISSN
  • 1176-9114 (ISSN-L)
  • 1178-2013
OpenAlex
W2800887222
Cited by
104
References
62
Citations by year
2026: 11 · 2025: 7 · 2024: 23 · 2023: 14 · 2022: 16 · 2021: 14 · 2020: 12 · 2019: 6 · 2018: 1
FWCI
3.30
Type
journal article
Language
en
Open access
Locations
  • International Journal of Nanomedicine (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
Funders
  • National Science Foundation (0934218)
  • Welch Foundation (AX-1615, -1615)
  • San Antonio Area Foundation (G12MD007591)
  • National Institutes of Health (AX-1615)
  • National Institute of General Medical Sciences (R25GM060655)
  • Division of Materials Research (DMR0934218, 0934218)
  • National Institute on Minority Health and Health Disparities (G12MD007591)
  • Air Force Office of Scientific Research (FA9550-, FA9550-15-1-0109)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Laser-Ablation Synthesis of Nanoparticles
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
Keywords
selenium nanoparticles, chitosan, Candida albicans, fungal biofilms, antifungal synergy, pulsed laser ablation in liquid, combination index, candidiasis, nanoparticle capping agents, surface sterilization
MeSH terms
Antifungal Agents, Candida albicans, Cell Line, Dose-Response Relationship, Drug, Drug Therapy, Combination, Humans, Lasers, Microbial Sensitivity Tests, Selenium, Biofilms, Nanotechnology, Chitosan, Nanoparticles

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