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An Electrochemical Cholesterol Biosensor Based on A CdTe/CdSe/ZnSe Quantum Dots—Poly (Propylene Imine) Dendrimer Nanocomposite Immobilisation Layer

Mokwebo, Kefilwe Vanessa, Oluwafemi, Oluwatobi Samuel, Arotiba, Omotayo Ademola·2018·Sensors
DOI 10.3390/s18103368
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Abstract

We report the preparation of poly (propylene imine) dendrimer (PPI) and CdTe/CdSe/ZnSe quantum dots (QDs) as a suitable platform for the development of an enzyme-based electrochemical cholesterol biosensor with enhanced analytical performance. The mercaptopropionic acid (MPA)-capped CdTe/CdSe/ZnSe QDs was synthesized in an aqueous phase and characterized using photoluminescence (PL) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), X-ray power diffraction (XRD), energy dispersive X-ray (EDX) spectroscopy. The absorption and emission maxima of the QDs red shifted as the reaction time and shell growth increased, indicating the formation of CdTe/CdSe/ZnSe QDs. PPI was electrodeposited on a glassy carbon electrode followed by the deposition (by deep coating) attachment of the QDs onto the PPI dendrimer modified electrode using 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) as a coupling agent. The biosensor was prepared by incubating the PPI/QDs modified electrode into a solution of cholesterol oxidase (ChOx) for 6 h. The modified electrodes were characterized by voltammetry and impedance spectroscopy. Since efficient electron transfer process between the enzyme cholesterol oxidase (ChOx) and the PPI/QDs-modified electrode was achieved, the cholesterol biosensor (GCE/PPI/QDs/ChOx) was able to detect cholesterol in the range 0.1–10 mM with a detection limit (LOD) of 0.075 mM and sensitivity of 111.16 μA mM−1 cm−2. The biosensor was stable for over a month and had greater selectivity towards the cholesterol molecule.

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Metadata

Authors
Mokwebo, K. V., Oluwafemi, O. S., & Arotiba, O. A.
Venue
Sensors
Publisher
MDPI AG
Published
2018-10-09
Volume
18
Issue
10
Pages
3368
ISSN
1424-8220
DOI
10.3390/s18103368
Cited by
50
References
1
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)
Funders
Department of Science and Technology and National Nanoscience Postgraduate Teaching and Training Programme (NNPTTP) (Grant)

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CoChat. (2026). Citation check: An Electrochemical Cholesterol Biosensor Based on A CdTe/CdSe/ZnSe Quantum Dots—Poly (Propylene Imine) Dendrimer Nanocomposite Immobilisation Layer. https://app.cochat.ai/papers/10.3390/s18103368
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Mokwebo, K. V., Oluwafemi, O. S., & Arotiba, O. A. (2018). An Electrochemical Cholesterol Biosensor Based on A CdTe/CdSe/ZnSe Quantum Dots—Poly (Propylene Imine) Dendrimer Nanocomposite Immobilisation Layer. Sensors. https://doi.org/10.3390/s18103368

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