The DOI matches its Crossref record; 67 of the 67 references it cites were found in a registry or an index.
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Open accessAbstract
Exosomes are small vesicles that mediate cell–cell communication. They contain proteins, lipids and RNA, and evidence is accumulating that these molecules are specifically sorted for release via exosomes. We recently showed that endothelial‐cell‐produced exosomes promote angiogenesis in vivo in a small RNA‐dependent manner. Recent deep sequencing studies in exosomes from lymphocytic origin revealed a broad spectrum of small RNAs. However, selective depletion or incorporation of small RNA species into endothelial exosomes has not been studied extensively. With next generation sequencing, we identified all known non‐coding RNA classes, including microRNAs (miRNAs), small nucleolar RNAs, yRNAs, vault RNAs, 5p and 3p fragments of miRNAs and miRNA‐like fragments. In addition, we mapped many fragments of messenger RNAs (mRNAs) and mitochondrial RNAs (mtRNAs). The distribution of small RNAs in exosomes revealed a considerable overlap with the distribution in the producing cells. However, we identified a remarkable enrichment of yRNA fragments and mRNA degradation products in exosomes consistent with yRNAs having a role in degradation of structured and misfolded RNAs in close proximity to endosomes. We propose that endothelial endosomes selectively sequester cytoplasmic RNA‐degrading machineries taking part in gene regulation. The release of these regulatory RNAs via exosomes may have implications for endothelial cell–cell communication.
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Cited by
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099
Self-citationThe biology , function , and biomedical applications of exosomes
Kalluri, R., & LeBleu, V. S. (2020) · Science · doi:10.1126/science.aau6977 · Cited by 10,770
RNA delivery by extracellular vesicles in mammalian cells and its applications
O’Brien, K., Breyne, K., Ughetto, S., Laurent, L. C., & Breakefield, X. O. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0251-y · Cited by 1,811
Extracellular Vesicles: Unique Intercellular Delivery Vehicles
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Methodological Guidelines to Study Extracellular Vesicles
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Metadata
- Authors
- van Balkom, B. W. M., Eisele, A. S., Pegtel, D. M., Bervoets, S., & Verhaar, M. C.
- Venue
- Journal of Extracellular Vesicles
- Publisher
- Wiley
- Published
- 2015-01
- Volume
- 4
- Issue
- 1
- Pages
- 26760-26760
- ISSN
- 2001-3078 (ISSN-L)
- OpenAlex
- W1769060942
- Cited by
- 298
- References
- 67
- FWCI
- 11.06
- Type
- journal article
- Language
- en
- Open access
- gold
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- van Balkom, B. W. M., Eisele, A. S., Pegtel, D. M., Bervoets, S., & Verhaar, M. C. (2015). Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting. Journal of Extracellular Vesicles. https://doi.org/10.3402/jev.v4.26760
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