The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A 16S rRNA-targeted Probe for Detection of Lactobacilli and Enterococci in Faecal Samples by Fluorescent In Situ Hybridization
Open accessAbstract
A group-specific 16S rRNA-targeted oligonucleotide probe S-G-Lab-0158-a-A20 (Lab158) was designed and validated to quantify species of the phylogenetic group lactobacilli-enterococci. The Lab158 probe detects nearly all species of the genera Lactobacillus, Enterococcus, Pediococcus, Weissella, Vagococcus, Leuconostoc and Oenococcus. The specificity of the probe was tested on various species of the target group and on a range of common intestinal bacteria. For these experiments, procedures to permeabilize these groups of Gram-positive bacteria were optimized and fluorescent in situ hybridization (FISH) conditions for maximum specificity were determined. In addition, we showed that it is possible to distinguish the predominant gut-enterococci i.e. E. faecalis among the Lab158 probe-positive cells with the E. faecalis
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Cites
A medium for the cultivation of lactobacilli
De MAN, J. C., ROGOSA, M., & SHARPE, M. E. (1960) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1960.tb00188.x · Cited by 5,311
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Microbiota and SCFA in lean and overweight healthy subjects
Schwiertz, A., Taras, D., Schäfer, K., Beijer, S., Bos, N. A., Donus, C., & Hardt, P. D. (2010) · Obesity · doi:10.1038/oby.2009.167 · Cited by 2,457
Distinct patterns of neonatal gut microflora in infants in whom atopy was and was not developing
Kalliomäki, M., Kirjavainen, P., Eerola, E., Kero, P., Salminen, S., & Isolauri, E. (2001) · Journal of Allergy and Clinical Immunology · doi:10.1067/mai.2001.111237 · Cited by 1,255
Mueller, S., Saunier, K., Hanisch, C., Norin, E., Alm, L., Midtvedt, T., Cresci, A., Silvi, S., Orpianesi, C., Verdenelli, M. C., et al. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.72.2.1027-1033.2006 · Cited by 1,006
Duncan, S. H., Belenguer, A., Holtrop, G., Johnstone, A. M., Flint, H. J., & Lobley, G. E. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02340-06 · Cited by 941
Swidsinski, A., Weber, J., Loening-Baucke, V., Hale, L. P., & Lochs, H. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.7.3380-3389.2005 · Cited by 894
Metadata
- Authors
- Hermie J. M. Harmsen, P. E.
- Venue
- Microbial Ecology in Health and Disease
- Publisher
- Informa UK Limited
- Published
- 1999-01
- Volume
- 11
- Issue
- 1
- Pages
- 3-12
- ISSN
- 0891-060X (ISSN-L)
- 1651-2235
- OpenAlex
- W2102686780
- Cited by
- 369
- References
- 1
- FWCI
- 3.48
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: A 16S rRNA-targeted Probe for Detection of Lactobacilli and Enterococci in Faecal Samples by Fluorescent In Situ Hybridization. https://app.cochat.ai/papers/10.1080/089106099435862
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- Hermie J. M. Harmsen, P. E. (1999). A 16S rRNA-targeted Probe for Detection of Lactobacilli and Enterococci in Faecal Samples by Fluorescent In Situ Hybridization. Microbial Ecology in Health and Disease. https://doi.org/10.1080/089106099435862
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