Cited by 3Antifungal resistance and susceptibilityFungal and yeast genetics researchFungal Infections and Studies

Synergetic action of atorvastatin and fluconazole against fluconazole-resistant Candida albicans in vitro and in a murine model for intra-abdominal Candidiasis / Ação sinérgica da atorvastatina e fluconazol contra Candida albicans resistente ao fluconazol in vitro e em um modelo murino contra Candidíase intra-abdominal

Open access
De Oliveira, Michael Éder, De Lima, William Gustavo, Alves, Carla Ferreira Bruno, Herrera, Karina Marjorie Silva, Santos, Patrícia Campi, De Souza, Daniele da Glória, Dos Santos, Maria Emilia Soares Martins, Ribeiro, Rosy Iara de Maciel de Azambuja +5 more·2022·Brazilian Journal of Development

Abstract

Introduction: Candida albicans is the most common causative agent of Intra-abdominal Candidiasis (IAC) and it is resistant to most antifungal drugs currently available. Here we investigated atorvastatin in vitro and in vivo antifungal activities against a fluconazole-resistant C. albicans strain as a potential repurposed drug. The following tests were carried out: antifungal susceptibility tests to determine minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), determination of time-kill curve, biofilm assays, Candida albicans yeast-hyphae transition inhibition assay, murine model of Intra-abdominal candidiasis, survival curve, fungal load quantification, histopathology analysis, quantification of TNF-α and IL-17 cytokines, quantification of N-acetyl-β-D-glucosaminidase. In vitro assays showed the synergetic action of atorvastatin and fluconazole against C. albicans growth and biofilm maturation while the time-kill curve assay revealed their fungicidal effect after 24 h of treatment. When yeast-to-hyphae transition was assessed, the synergetic effect of atorvastatin and fluconazole reduced C. albicans filamentation significantly. In vivo tests showed that one of the most noticeable signs of IAC is the intense systemic inflammation. However, our survival curve test showed that despite being ill, animals exhibited little to no clinical signs of systemic inflammation when treatment included a combination of atorvastatin and fluconazole. Altogether, these findings suggest that atorvastatin could be feasibly used in the treatment fluconazole-resistant C. albicans strains, showing that drug repurposing is an important strategy when considering the limited number of antifungal drugs available for treatment in addition to financial hardship experienced in research and development of new antifungal drugs.

Integrity signals

Registry

Crossref · 10.34117/bjdv8n5-543

Cites

37 of 37 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Fluconazole and ATV combinations show fungicidal activity within 24 h against fluconazole-resistant C. albicans, significantly inhibiting biofilm maturation and filamentation.

    “Likewise, fluconazole and ATV combinations have shown fungicidal activity within 24 h against fluconazole‐resistant C. albicans , significantly inhibiting biofilm maturation and filamentation [126].”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
De Oliveira, M. É., De Lima, W. G., Alves, C. F. B., Herrera, K. M. S., Santos, P. C., De Souza, D. D. G., Dos Santos, M. E. S. M., Ribeiro, R. I. D. M. D. A., Thomé, R. G., Dos Santos, H. B., Soares, A. C., Soares-Martins, J. A. P., & Ferreira, J. M. S.
Affiliations
Universidade Federal de Minas Gerais (BR)
Venue
Brazilian Journal of Development
Published
2022-05-26
Volume
8
Issue
5
Pages
41115-41137
ISSN
2525-8761 (ISSN-L)
OpenAlex
W4281746376
Cited by
3
References
37
Citations by year
2025: 2 · 2023: 1
FWCI
0.37
Type
journal article
Language
en
Open access
Locations
  • Brazilian Journal of Development (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Fundação de Amparo à Pesquisa do Estado de Minas Gerais
  • Universidade Federal de São João del-Rei
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords
atorvastatin, fluconazole, Candida albicans, fluconazole resistance, intra-abdominal candidiasis, drug repurposing, antifungal synergy, biofilm, yeast-to-hypha transition, filamentation, systemic inflammation, TNF-α, IL-17, N-acetyl-beta-D-glucosaminidase, mouse model

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review