The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Construction of a Portal Implantable Functional Tissue-Engineered Liver Using Perfusion-Decellularized Matrix and Hepatocytes in Rats
Open accessAbstract
Innovative cell-based therapies, including hepatic tissue engineering following hepatocyte transplantation, are considered as theoretical alternatives to liver transplant or for partial replacement of liver function in patients. However, recent progress in hepatic tissue engineering has been hampered by low initial hepatocyte engraftment and insufficient blood supply in vivo. We developed an intact 3D scaffold of an extracellular matrix (ECM) derived from a decellularized liver lobe, with layer-by-layer (LbL) heparin deposition to avoid thrombosis, which we repopulated with hepatocytes and successfully implanted as a tissue-engineered liver (TEL) into the portal system. The TEL provided sufficient volume for transplantation of cell numbers representing up to 10% of whole-liver equivalents and was perfused by portal vein blood. Treatment of extended hepatectomized rats with a TEL improved liver function and prolonged survival; mean lifespan was extended from 16 to 72 h. At 72 h postoperation, the TEL sustained functional and viable hepatocytes. In conclusion, we propose the TEL as a state-of-the-art substitute for whole-liver transplantation and as a proof of concept for the technology that will eventually allow for the transplantation of a reconstituted liver.
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In the citing paper
“Transplanted grafts supported hepatic function for 72 h, preserving hepatocyte morphology and prolonging animal survival.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [218]
In the citing paper
“Bao et al. were the first to describe heparin immobilization within rat liver scaffold, primarily for reducing coagulation in vivo rather than improving reendothelialization efficiency [218].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [218]
In the citing paper
“The transplanted scaffold improved liver function, decreased blood ammonia levels, and prolonged survival compared to a 90 % hepatectomy.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [218]
Citations
Cites
1 retracted
RETRACTED: Clinical transplantation of a tissue-engineered airway
Macchiarini, P., Jungebluth, P., Go, T., Asnaghi, M. A., Rees, L., Cogan, T. A., Dodson, A., Martorell, J., Bellini, S., Parnigotto, P. P., et al. (2008) · The Lancet · doi:10.1016/s0140-6736(08)61598-6 · Cited by 1,431
RetractedPerfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Ott, H. C., Matthiesen, T. S., Goh, S.-K., Black, L. D., Kren, S. M., Netoff, T. I., & Taylor, D. A. (2008) · Nature Medicine · doi:10.1038/nm1684 · Cited by 2,650
Derivation of embryonic germ cells and male gametes from embryonic stem cells
Geijsen, N., Horoschak, M., Kim, K., Gribnau, J. H., Eggan, K. C., & Daley, G. Q. (2003) · Nature · doi:10.1038/nature02247 · Cited by 860
Engineering functional two- and three-dimensional liver systems in vivo using hepatic tissue sheets
Ohashi, K., Yokoyama, T., Yamato, M., Kuge, H., Kanehiro, H., Tsutsumi, M., Amanuma, T., Iwata, H., Yang, J. Y., Okano, T., et al. (2007) · Nature Medicine · doi:10.1038/nm1576 · Cited by 509
Decellularized Native and Engineered Arterial Scaffolds for Transplantation
Dahl, S. L. M., Koh, J., Prabhakar, V., & Niklason, L. E. (2003) · Cell Transplantation · doi:10.3727/000000003108747136 · Cited by 406
Replacement of Liver Function in Rats by Transplantation of Microcarrier-Attached Hepatocytes
Demetriou, A. A., Whiting, J. F., Feldman, D., Levenson, S. Μ., Chowdhury, N. R., Moscioni, A. D., Kram, M. T., & Chowdhury, J. R. (1986) · Science · doi:10.1126/science.2426782 · Cited by 294
A Bioartificial Liver--State of the Art
Strain, A. J., & Neuberger, J. (2002) · Science · doi:10.1126/science.1068660 · Cited by 285
Wang, B., Liu, P., Jiang, W., Pan, H., Xu, X., & Tang, R. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200704718 · Cited by 245
Schlosser, S. F., Burgstahler, A. D., & Nathanson, M. H. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.18.9948 · Cited by 225
Soto–Gutiérrez, A., Kobayashi, N., Rivas‐Carrillo, J. D., Navarro–Álvarez, N., Zhao, D., Okitsu, T., Noguchi, H., Basma, H., Tabata, Y., Chen, Y., et al. (2006) · Nature Biotechnology · doi:10.1038/nbt1257 · Cited by 218
Research works printed but not linked: 2
- Kang Y. Z., 2009, Zhonghua Yi Xue Za Zhi, 89, 1135
- Uygun B. E., 2008, Making of a new liver using decellularized livers as scaffolds (Abstract).
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Chandra, P. K., & Atala, A. A. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-801238-3.11078-5 · Cited by 6
Nelson, V. L., Stumbras, A. R., Palumbo, R. N., Riesgraf, S. A., Balboa, M. S., Hannah, Z. A., Bergstrom, I. J., Fecteau, C., Lake, J. R., Barry, J. J., et al. (2023) · Bioengineering · doi:10.3390/bioengineering10101201 · Cited by 4
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Bobrova, M. M., Safonova, L. A., Efimov, А. Е., Iljinsky, I. M., Агапова, О. И., & Agapov, I. I. (2021) · Biomedical Materials · doi:10.1088/1748-605x/ac058b · Cited by 4
Kwon, T., & Moon, K. H. (2017) · Springer eBooks · doi:10.1007/978-981-10-2723-9_6 · Cited by 3
Sakamoto, H., Shirakigawa, N., Bual, R. P., Fukuda, Y., Nakamura, S., Miyata, T., Yamao, T., Yamashita, Y., Baba, H., & Ijima, H. (2019) · Journal of Artificial Organs · doi:10.1007/s10047-019-01106-6 · Cited by 2
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Cho, C. H. (2014) · Hanyang Medical Reviews · doi:10.7599/hmr.2014.34.4.153 · Cited by 1
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Kim, M. J., Chi, B. H., Cho, M. J., Whang, Y. M., & Chang, I. H. (2017) · Journal of Urologic Oncology · doi:10.22465/kjuo.2017.15.2.51 · Cited by 1
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Franco, D., & Si‐Tayeb, K. (2011) · Bulletin de l Académie Nationale de Médecine · doi:10.1016/s0001-4079(19)31960-0 · Cited by 1
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Wang, A., Jank, I., Wei, W., Schindler, C., & Dahmen, U. (2018) · Journal of Visualized Experiments · doi:10.3791/57991 · Cited by 1
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Sauer, I. M., Raschzok, N., & Neuhaus, P. (2014) · doi:10.1002/9781118873434.ch47 · Cited by 1
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Metadata
- Authors
- Bao, J., Shi, Y., Sun, H., Yin, X., Yang, R., Li, L., Chen, X., & Bu, H.
- Affiliations
- Sichuan University (CN)
- Venue
- Cell Transplantation
- Publisher
- SAGE Publications
- Published
- 2011-06
- Volume
- 20
- Issue
- 5
- Pages
- 753-766
- ISSN
- 0963-6897 (ISSN-L)
- 1555-3892
- OpenAlex
- W1965557044
- Cited by
- 133
- References
- 29
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 8 · 2022: 3 · 2021: 12 · 2020: 6 · 2019: 13 · 2018: 9 · 2017: 15 · 2016: 14 · 2015: 14 · 2014: 12 · 2013: 12 · 2012: 5
- FWCI
- 7.09
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cell Transplantation (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- hepatocyte transplantation, extracellular matrix scaffolds, heparin coating, thromboprophylaxis, portal vein perfusion, extended hepatectomy, liver transplantation alternatives, hepatocyte engraftment, rat model
- MeSH terms
- Animals, Extracellular Matrix, Liver Diseases, Male, Portal Vein, Rats, Inbred Lew, Liver Transplantation, Liver, Artificial, Hepatocytes, Tissue Engineering, Rats
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