Comparison of Cardiomyogenic Potential among Human ESC and iPSC Lines
Open accessAbstract
We recently reported that, following induction of clumps of pluripotent H1 human embryonic stem cells (hESCs) with activin-A and Bmp4 in defined medium for 5 days, widespread differentiation of rhythmically contracting cardiomyocytes occurs within 3–4 weeks. In this study, the same approach was used to assess whether human induced pluripotent stem cells (hiPSCs), which may theoretically provide an unlimited source of patient-matched cells for transplantation therapy, can similarly undergo cardiomyocyte differentiation. Differentiation of four pluripotent cell lines (H1 and H9 hESCs and C2a and C6a hiPSCs) was compared in parallel by monitoring rhythmic contraction, morphologic differentiation, and expression of cardiomyogenic genes. Based on expression of the cardiomyogenic lineage markers MESP1, ISL1, and NKX2-5, all four cell lines were induced into the cardiomyogenic lineage. However, in contrast to the widespread appearance of striations and rhythmic contractility seen in H9 and especially in H1 hESCs, both hiPSC lines exhibited poor terminal differentiation. These findings suggest that refined modes of generating hiPSCs, as well as of inducing cardiomyogenesis in them, may be required to fulfill their potential as agents of cardiac regeneration.
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At present, the 3′ UTR is mainly derived from hemoglobin subunit α (HBA) and subunit β (HBB) genes, but it can also be derived from albumin (ALB), heat-shock protein 70 (Hsp70), tyrosine hydroxylase (TH), and collagen alpha 1 (COL1A1) genes.
“At present, the 3′ UTR is mainly derived from hemoglobin subunit α (HBA) and subunit β (HBB) genes,140 but it can also be derived from albumin (ALB), heat-shock protein 70 (Hsp70), tyrosine hydroxylase (TH), and collagen alpha 1 (COL1A1) genes.141–143”
From Advances in COVID-19 mRNA vaccine development (2022) · cites this paper as [143]
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Fang, E., Liu, X., Li, M., Zhang, Z., Song, L., Zhu, B., Wu, X., Liu, J., Zhao, D., & Li, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00950-y · Cited by 601 · see citing works
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- Authors
- Sepac, A., Si-Tayeb, K., Sedlic, F., Barrett, S., Canfield, S., Duncan, S. A., Bosnjak, Z. J., & Lough, J. W.
- Affiliations
- Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, USA
- Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI, USA
- Medical College of Wisconsin (US)
- Venue
- Cell Transplantation
- Publisher
- SAGE Publications
- Published
- 2012-11
- Volume
- 21
- Issue
- 11
- Pages
- 2523-2530
- ISSN
- 0963-6897 (ISSN-L)
- 1555-3892
- PMC
- PMC3522765
- OpenAlex
- W1975410103
- Cited by
- 36
- References
- 18
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 5 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 7 · 2013: 2
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Cell Transplantation (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Heart, Lung, and Blood Institute (HL089471, HL034708, P01HL089471, R01HL034708)
- National Institute of General Medical Sciences (P01GM066730, GM-066730)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- cardiomyocyte differentiation, human induced pluripotent stem cells, human embryonic stem cells, activin A, BMP4, Mesp1, Isl1, Nkx2.5, cardiac regeneration
- MeSH terms
- Cell Differentiation, Cell Line, Humans, Myocytes, Cardiac, Embryonic Stem Cells, Induced Pluripotent Stem Cells
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