Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine
Abstract
The field of gene therapy has evolved over the past two decades after the first introduction of nucleic acid drugs, such as plasmid DNA (pDNA). With the development of in vitro transcription (IVT) methods, synthetic mRNA has become an emerging class of gene therapy. IVT mRNA has several advantages over conventional pDNA for the expression of target proteins. mRNA does not require nuclear localization to mediate protein translation. The intracellular process for protein expression is much simpler and there is no potential risk of insertion mutagenesis. Having these advantages, the level of protein expression is far enhanced as comparable to that of viral expression systems. This makes IVT mRNA a powerful alternative gene expression system for various applications in regenerative medicine. In this review, we highlight the synthesis and preparation of IVT mRNA and its therapeutic applications. The article includes the design and preparation of IVT mRNA, chemical modification of IVT mRNA, and therapeutic applications of IVT mRNA in cellular reprogramming, stem cell engineering, and protein replacement therapy. Finally, future perspectives and challenges of IVT mRNA are discussed.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The 5′-cap structure prevents mRNA from degradation by exonucleases, thereby maintaining mRNA stability and enabling translation initiation.
“The 5′-cap structure prevents mRNA from degradation by exonucleases, thereby maintaining mRNA stability and enabling translation initiation.115”
From Advances in COVID-19 mRNA vaccine development (2022) · cites this paper as [115]
Co-transcriptional capping is the most commonly used method in IVT for mRNA.
“Co-transcriptional capping is the most commonly used method in IVT for mRNA,115”
From Advances in COVID-19 mRNA vaccine development (2022) · cites this paper as [115]
Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673 · see citing works
Global quantification of mammalian gene expression control
Schwanhäusser, B., Busse, D., Li, N., Dittmar, G., Schuchhardt, J., Wolf, J., Chen, W., & Selbach, M. (2011) · Nature · doi:10.1038/nature10098 · Cited by 6,566 · see citing works
Direct Gene Transfer into Mouse Muscle in Vivo
Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L. (1990) · Science · doi:10.1126/science.1690918 · Cited by 4,173 · see citing works
Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8
Heil, F., Hemmi, H., Hochrein, H., Ampenberger, F., Kirschning, C., Akira, S., Lipford, G., Wagner, H., & Bauer, S. (2004) · Science · doi:10.1126/science.1093620 · Cited by 3,947 · see citing works
Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA
Diebold, S. S., Kaisho, T., Hemmi, H., Akira, S., & Reis e Sousa, C. (2004) · Science · doi:10.1126/science.1093616 · Cited by 3,444 · see citing works
Non-viral vectors for gene-based therapy
Yin, H., Kanasty, R. L., Eltoukhy, A. A., Vegas, A. J., Dorkin, J. R., & Anderson, D. G. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3763 · Cited by 3,123 · see citing works
Karikó, K., Buckstein, M., Ni, H., & Weissman, D. (2005) · Immunity · doi:10.1016/j.immuni.2005.06.008 · Cited by 2,728 · see citing works
Warren, L., Manos, P. D., Ahfeldt, T., Loh, Y.-H., Li, H., Lau, F., Ebina, W., Mandal, P. K., Smith, Z. D., Meissner, A., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.08.012 · Cited by 2,662 · see citing works
mRNA-based therapeutics--developing a new class of drugs
Sahin, U., Karikó, K., & Türeci, Ö. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4278 · Cited by 2,474 · see citing works
Production of recombinant protein therapeutics in cultivated mammalian cells
Wurm, F. M. (2004) · Nature Biotechnology · doi:10.1038/nbt1026 · Cited by 2,032 · see citing works
Karikó, K., Muramatsu, H., Welsh, F. A., Ludwig, J., Kato, H., Akira, S., & Weissman, D. (2008) · Molecular Therapy · doi:10.1038/mt.2008.200 · Cited by 1,930 · see citing works
Protein Therapeutics: A Summary and Pharmacological Classification
Leader, B., Baca, Q. J., & Golan, D. E. (2008) · Nature Reviews Drug Discovery · doi:10.1038/nrd2399 · Cited by 1,913 · see citing works
Generation of Induced Pluripotent Stem Cells Using Recombinant Proteins
Zhou, H., Wu, S., Joo, J. Y., Zhu, S., Han, D. W., Lin, T., Trauger, S., Bien, G., Yao, S., Zhu, Y., et al. (2009) · Cell stem cell · doi:10.1016/j.stem.2009.04.005 · Cited by 1,788 · see citing works
Induced pluripotent stem cells generated without viral integration
Stadtfeld, M., Nagaya, M., Utikal, J., Weir, G., & Hochedlinger, K. (2008) · Science · doi:10.1126/science.1162494 · Cited by 1,683 · see citing works
Synonymous but not the same: the causes and consequences of codon bias
Plotkin, J. B., & Kudla, G. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2899 · Cited by 1,677 · see citing works
Lee, K., Silva, E. A., & Mooney, D. J. (2010) · Journal of The Royal Society Interface · doi:10.1098/rsif.2010.0223 · Cited by 1,393 · see citing works
Self-citationIn vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes
Li, Q., Huang, Y., Spencer, C. I., Foley, A., Vedantham, V., Liu, L., Conway, S. J., Fu, J., & Srivastava, D. (2012) · Nature · doi:10.1038/nature11044 · Cited by 1,353 · see citing works
At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells
Kozak, M. (1987) · Journal of Molecular Biology · doi:10.1016/0022-2836(87)90418-9 · Cited by 1,182 · see citing works
Leukocyte Migration into Inflamed Tissues
Nourshargh, S., & Alon, R. (2014) · Immunity · doi:10.1016/j.immuni.2014.10.008 · Cited by 1,172 · see citing works
Heart repair by reprogramming non-myocytes with cardiac transcription factors
Song, K., Nam, Y., Luo, X., Qi, X., Tan, W. H., Huang, G. N., Acharya, A., Smith, C. L., Tallquist, M. D., Neilson, E. G., et al. (2012) · Nature · doi:10.1038/nature11139 · Cited by 1,141 · see citing works
Protein drug stability: a formulation challenge
Frøkjær, S., & Otzen, D. E. (2005) · Nature Reviews Drug Discovery · doi:10.1038/nrd1695 · Cited by 1,102 · see citing works
mRNA is an endogenous ligand for toll-like receptor 3
Karikó, K., Ni, H., Capodici, J., Lamphier, M., & Weissman, D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310175200 · Cited by 1,062 · see citing works
Type 1 Diabetes: Etiology, Immunology, and Therapeutic Strategies
Van Belle, T. L., Coppieters, K. T., & Von Herrath, M. G. (2011) · Physiological Reviews · doi:10.1152/physrev.00003.2010 · Cited by 1,022 · see citing works
Untranslated regions of mRNAs.
(2002) · Genome biology · doi:10.1186/gb-2002-3-3-reviews0004 · Cited by 987 · see citing works
Regenerative medicine: Current therapies and future directions
Mao, A. S., & Mooney, D. J. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1508520112 · Cited by 960 · see citing works
Modified mRNA vaccines protect against zika virus infection
Richner, J. M., Himansu, S., Dowd, K. A., Butler, S. L., Salazar, V., Fox, J. M., Julander, J. G., Tang, W. W., Shresta, S., Pierson, T. C., et al. (2017) · Cell · doi:10.1016/j.cell.2017.02.017 · Cited by 909 · see citing works
Karikó, K., Muramatsu, H., Ludwig, J., & Weissman, D. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr695 · Cited by 898 · see citing works
mRNA capping: biological functions and applications
Ramanathan, A., Robb, G. B., & Chan, S. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw551 · Cited by 892 · see citing works
Therapeutic antibodies for autoimmunity and inflammation
Chan, A. C., & Carter, P. J. (2010) · Nature reviews. Immunology · doi:10.1038/nri2761 · Cited by 840 · see citing works
A decade of transcription factor-mediated reprogramming to pluripotency
Takahashi, K., & Yamanaka, S. (2016) · Nature reviews. Molecular cell biology · doi:10.1038/nrm.2016.8 · Cited by 815 · see citing works
Basics and Recent Advances in Peptide and Protein Drug Delivery
Bruno, B. J., Miller, G. D., & Lim, C. S. (2013) · Therapeutic Delivery · doi:10.4155/tde.13.104 · Cited by 760 · see citing works
Expression of therapeutic proteins after delivery of chemically modified mRNA in mice
Kormann, M. S. D., Hasenpusch, G., Aneja, M. K., Nica, G., Flemmer, A. W., Herber-Jonat, S., Huppmann, M., Mays, L. E., Illenyi, M., Schams, A., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1733 · Cited by 733 · see citing works
Improvement in outcomes of clinical islet transplantation: 1999–2010
Barton, F. B., Rickels, M. R., Alejandro, R., Hering, B. J., Wease, S., Naziruddin, B., Oberholzer, J., Odorico, J. S., Garfinkel, M. R., Levy, M., et al. (2012) · Diabetes Care · doi:10.2337/dc12-0063 · Cited by 725 · see citing works
Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration
Jopling, C., Boué, S., & Belmonte, J. C. I. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3043 · Cited by 724 · see citing works
Lipid Nanoparticle Assisted mRNA Delivery for Potent Cancer Immunotherapy
Oberli, M. A., Reichmuth, A. M., Dorkin, J. R., Mitchell, M. J., Fenton, O. S., Jaklenec, A., Anderson, D. G., Langer, R., & Blankschtein, D. (2017) · Nano Letters · doi:10.1021/acs.nanolett.6b03329 · Cited by 719 · see citing works
Andries, O., Mc Cafferty, S., De Smedt, S. C., Weiss, R., Sanders, N. N., & Kitada, T. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.08.051 · Cited by 701 · see citing works
Masuda, N., Ohnishi, T., Kawamoto, S., Monden, M., & Okubo, K. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.22.4436 · Cited by 683 · see citing works
Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation
Anderson, B. R., Muramatsu, H., Nallagatla, S. R., Bevilacqua, P. C., Sansing, L. H., Weissman, D., & Karikó, K. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq347 · Cited by 682 · see citing works
The mRNA 5′ cap-binding protein eIF4E and control of cell growth
Sonenberg, N., & Gingras, A. (1998) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(98)80150-6 · Cited by 673 · see citing works
Zangi, L., Lui, K. O., von Gise, A., Ma, Q., Ebina, W., Ptaszek, L. M., Später, D., Xu, H., Tabebordbar, M., Gorbatov, R., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2682 · Cited by 646 · see citing works
Holtkamp, S., Kreiter, S., Selmi, A., Simon, P., Koslowski, M., Huber, C., Türeci, O., & Sahin, U. (2006) · Blood · doi:10.1182/blood-2006-04-015024 · Cited by 625 · see citing works
Antitumor effect of genetically engineered mesenchymal stem cells in a rat glioma model
Nakamura, K., Ito, Y., Kawano, Y., Kurozumi, K., Kobune, M., Tsuda, H., Bizen, A., Honmou, O., Niitsu, Y., & Hamada, H. (2004) · Gene Therapy · doi:10.1038/sj.gt.3302276 · Cited by 597 · see citing works
Delivery technologies for genome editing
Yin, H., Kauffman, K. J., & Anderson, D. G. (2017) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.280 · Cited by 597 · see citing works
Recognition of cap structure in splicing in vitro of mRNA precursors
Konarska, M. M., Padgett, R. A., & Sharp, P. A. (1984) · Cell · doi:10.1016/0092-8674(84)90268-x · Cited by 588 · see citing works
Rezania, A., Bruin, J. E., Riedel, M. J., Mojibian, M., Asadi, A., Xu, J., Gauvin, R., Narayan, K., Karanu, F., O’Neil, J. J., et al. (2012) · Diabetes · doi:10.2337/db11-1711 · Cited by 582 · see citing works
Mesenchymal stem cells display coordinated rolling and adhesion behavior on endothelial cells
Rüster, B., Göttig, S., Ludwig, R. J., Bistrian, R., Müller, S., Seifried, E., Gille, J., & Henschler, R. (2006) · Blood · doi:10.1182/blood-2006-05-025098 · Cited by 562 · see citing works
Nucleic-acid therapeutics: basic principles and recent applications
Opalinska, J., & Gewirtz, A. M. (2002) · Nature Reviews Drug Discovery · doi:10.1038/nrd837 · Cited by 535 · see citing works
Essential Role for the dsRNA-Dependent Protein Kinase PKR in Innate Immunity to Viral Infection
Balachandran, S., Roberts, P. C., Brown, L. E., Truong, H., Pattnaik, A. K., Archer, D. R., & Barber, G. N. (2000) · Immunity · doi:10.1016/s1074-7613(00)00014-5 · Cited by 527 · see citing works
Characterization and Functionality of Cell Surface Molecules on Human Mesenchymal Stem Cells
Majumdar, M. K., Keane-Moore, M., Buyaner, D., Hardy, W., Moorman, M. A., McIntosh, K. R., & Mosca, J. D. (2003) · Journal of Biomedical Science · doi:10.1007/bf02256058 · Cited by 527 · see citing works
Kahvejian, A., Svitkin, Y. V., Sukarieh, R., M'Boutchou, M.-N., & Sonenberg, N. (2005) · Genes & Development · doi:10.1101/gad.1262905 · Cited by 491 · see citing works
Ryter, J. M., & Schultz, S. C. (1998) · The EMBO Journal · doi:10.1093/emboj/17.24.7505 · Cited by 479 · see citing works
5'-terminal cap structure in eucaryotic messenger ribonucleic acids
Banerjee, A. K. (1980) · Microbiological Reviews · doi:10.1128/mr.44.2.175-205.1980 · Cited by 477 · see citing works
Zhao, Y., Moon, E. K., Carpenito, C., Paulos, C. M., Liu, X., Brennan, A. L., Chew, A., Carroll, R. G., Scholler, J., Levine, B. L., et al. (2010) · Cancer Research · doi:10.1158/0008-5472.can-10-2880 · Cited by 448 · see citing works
Cheng, Z., Ou, L., Zhou, X., Li, F., Jia, X., Zhang, Y., Liu, X., Li, Y., Ward, C. A., Melo, L. G., et al. (2008) · Molecular Therapy · doi:10.1038/sj.mt.6300374 · Cited by 440 · see citing works
Alton, E. W. F. W., Stern, M., Farley, R., Jaffé, A., Chadwick, S., Phillips, J., Davies, J. C., Smith, S. N., Browning, J. A., Davies, M., et al. (1999) · The Lancet · doi:10.1016/s0140-6736(98)06532-5 · Cited by 434 · see citing works
Cellular IRES-mediated translation
Komar, A. A., & Hatzoglou, M. (2011) · Cell Cycle · doi:10.4161/cc.10.2.14472 · Cited by 418 · see citing works
Elroy‐Stein, O., Fuerst, T. R., & Moss, B. A. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.16.6126 · Cited by 405 · see citing works
Getting Leukocytes to the Site of Inflammation
Müller, W. A. (2013) · Veterinary Pathology · doi:10.1177/0300985812469883 · Cited by 401 · see citing works
Gene therapy progress and prospects: non-viral gene therapy by systemic delivery
Li, S.-D., & Huang, L. (2006) · Gene Therapy · doi:10.1038/sj.gt.3302838 · Cited by 392 · see citing works
Nanotechnologies in delivery of mRNA therapeutics using nonviral vector-based delivery systems
Guan, S., & Rosenecker, J. (2017) · Gene Therapy · doi:10.1038/gt.2017.5 · Cited by 389 · see citing works
Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor
Wood, A. J. J., Lieschke, G. J., & Burgess, A. W. (1992) · New England Journal of Medicine · doi:10.1056/nejm199207093270207 · Cited by 373 · see citing works
XRN 5′→3′ exoribonucleases: Structure, mechanisms and functions
Nagarajan, V. K., Jones, C. I., Newbury, S. F., & Green, P. J. (2013) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2013.03.005 · Cited by 366 · see citing works
Matoulkova, E., Michalova, E., Vojtesek, B., & Hrstka, R. (2012) · RNA Biology · doi:10.4161/rna.20231 · Cited by 357 · see citing works
(2001) · PubMed Central · Cited by 342 · see citing works
Chamary, J., & Hurst, L. D. (2005) · Genome biology · doi:10.1186/gb-2005-6-9-r75 · Cited by 332 · see citing works
Advances in Non-Viral DNA Vectors for Gene Therapy
Hardee, C. L., Arévalo-Soliz, L., Hornstein, B. D., & Zechiedrich, L. (2017) · Genes · doi:10.3390/genes8020065 · Cited by 329 · see citing works
Carey, B. W., Markoulaki, S., Hanna, J. H., Faddah, D. A., Buganim, Y., Kim, J., Ganz, K., Steine, E. J., Cassady, J. P., Creyghton, M. P., et al. (2011) · Cell stem cell · doi:10.1016/j.stem.2011.11.003 · Cited by 326 · see citing works
Efficient generation of human iPSCs by a synthetic self-replicative RNA
Yoshioka, N., Gros, E., Li, H.-R., Kumar, S., Deacon, D. C., Maron, C., Muotri, A. R., Chi, N. C., Fu, X.-D., Yu, B. D., et al. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.06.001 · Cited by 312 · see citing works
Kreiter, S., Selmi, A., Diken, M., Koslowski, M., Britten, C. M., Huber, C., Türeci, Ö., & Sahin, U. (2010) · Cancer Research · doi:10.1158/0008-5472.can-10-0699 · Cited by 309 · see citing works
Karikó, K., Muramatsu, H., Keller, J. M., & Weissman, D. (2012) · Molecular Therapy · doi:10.1038/mt.2012.7 · Cited by 282 · see citing works
Diebold, S. S., Massacrier, C., Akira, S., Paturel, C., Morel, Y., & Sousa, C. R. E. (2006) · European Journal of Immunology · doi:10.1002/eji.200636617 · Cited by 281 · see citing works
Genetic Disorders of Surfactant Dysfunction
Wert, S. E., Whitsett, J. A., & Nogee, L. M. (2009) · Pediatric and Developmental Pathology · doi:10.2350/09-01-0586.1 · Cited by 280 · see citing works
Regulation of liver regeneration by growth factors and cytokines
Böhm, F., Köhler, U. A., Speicher, T., & Werner, S. (2010) · EMBO Molecular Medicine · doi:10.1002/emmm.201000085 · Cited by 275 · see citing works
Poole, E. S., Brown, C. M., & Tate, W. P. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb06985.x · Cited by 267 · see citing works
The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases
Liu, H., Rodgers, N. D., Jiao, X., & Kiledjian, M. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf448 · Cited by 267 · see citing works
Wang, Y., Deng, Y., & Zhou, G. (2008) · Brain Research · doi:10.1016/j.brainres.2007.11.068 · Cited by 266 · see citing works
Novel “anti-reverse” cap analogs with superior translational properties
Jemielity, J., Fowler, T. E., Zuberek, J., Stȩpiński, J., Lewdorowicz, M., Niedźwiecka-Kornaś, A., Stolarski, R., Darżynkiewicz, E., & Rhoads, R. E. (2003) · RNA · doi:10.1261/rna.5430403 · Cited by 263 · see citing works
An mRNA Stability Complex Functions with Poly(A)-Binding Protein To Stabilize mRNA In Vitro
Wang, Z., Day, N., Trifillis, P., & Kiledjian, M. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.7.4552 · Cited by 262 · see citing works
Engineering mesenchymal stem cells for regenerative medicine and drug delivery
Park, J. S., Suryaprakash, S., Lao, Y., & Leong, K. W. (2015) · Methods · doi:10.1016/j.ymeth.2015.03.002 · Cited by 242 · see citing works
Yakubov, E., Rechavi, G., Rozenblatt, S., & Givol, D. (2010) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.02.150 · Cited by 240 · see citing works
Non-viral gene therapy: Gains and challenges of non-invasive administration methods
Földvári, M., Chen, D.-W., Nafissi, N., Calderon-Nieva, D., Narsineni, L., & Rafiee, A. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.12.012 · Cited by 240 · see citing works
Characterization of CD4+ T Cell Responses During Natural Infection with Salmonella typhimurium
McSorley, S. J., Cookson, B. T., & Jenkins, M. K. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.164.2.986 · Cited by 239 · see citing works
Characterization of key mechanisms in transmigration and invasion of mesenchymal stem cells
Steingen, C., Brenig, F., Baumgartner, L., Schmidt, J., Schmidt, A., & Bloch, W. (2008) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2008.03.010 · Cited by 233 · see citing works
Chang, C.-W., Lai, Y.-S., Pawlik, K. M., Liu, K.-M., Sun, C.-W., Li, C., Schoeb, T., & Townes, T. (2009) · Stem Cells · doi:10.1002/stem.39 · Cited by 229 · see citing works
Inefficient Reprogramming of Fibroblasts into Cardiomyocytes Using Gata4, Mef2c, and Tbx5
Chen, J., Krane, M., Deutsch, M.-A., Wang, L., Rav-Acha, M., Grégoire, S., Engels, M. C., Rajarajan, K., Karra, R., Abel, E. D., et al. (2012) · Circulation Research · doi:10.1161/circresaha.112.270264 · Cited by 228 · see citing works
Kuhn, A. N., Diken, M., Kreiter, S., Selmi, A., Kowalska, J., Jemielity, J., Darzynkiewicz, E., Huber, C., Türeci, Ö., & Sahin, U. (2010) · Gene Therapy · doi:10.1038/gt.2010.52 · Cited by 226 · see citing works
Increased Antigen Presentation Efficiency by Coupling Antigens to MHC Class I Trafficking Signals
Kreiter, S., Selmi, A., Diken, M., Sebastian, M., Osterloh, P., Schild, H., Huber, C., Türeci, Ö., & Şahin, U. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.180.1.309 · Cited by 221 · see citing works
Modified mRNA Vaccines Protect against Zika Virus Infection
Richner, J. M., Himansu, S., Dowd, K. A., Butler, S. L., Salazar, V., Fox, J. M., Julander, J. G., Tang, W. W., Shresta, S., Pierson, T. C., et al. (2017) · Cell · doi:10.1016/j.cell.2017.03.016 · Cited by 221 · see citing works
Induced Pluripotent Stem Cells: Problems and Advantages when Applying them in Regenerative Medicine
Medvedev, S., Shevchenko, A. I., & Zakian, S. (2010) · Acta Naturae · doi:10.32607/20758251-2010-2-2-18-27 · Cited by 220 · see citing works
Levy, O., Zhao, W., Mortensen, L. J., LeBlanc, S., Tsang, K., Fu, M., Phillips, J. A., Sagar, V., Anandakumaran, P. N., Ngai, J., et al. (2013) · Blood · doi:10.1182/blood-2013-04-495119 · Cited by 212 · see citing works
Functions of the cytoplasmic RNA sensors RIG-I and MDA-5: Key regulators of innate immunity
Barral, P. M., Sarkar, D., Su, Z.-Z., Barber, G. N., DeSalle, R., Racaniello, V. R., & Fisher, P. B. (2009) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2009.06.012 · Cited by 200 · see citing works
Xia, L., McDaniel, J. M., Yago, T., Doeden, A., & McEver, R. P. (2004) · Blood · doi:10.1182/blood-2004-02-0650 · Cited by 199 · see citing works
Comprehensive Analysis of Chemotactic Factors for Bone Marrow Mesenchymal Stem Cells
Ozaki, Y., Nishimura, M., Sekiya, K., Suehiro, F., Kanawa, M., Nikawa, H., Hamada, T., & Kato, Y. (2007) · Stem Cells and Development · doi:10.1089/scd.2006.0032 · Cited by 198 · see citing works
Kauffman, K. J., Mir, F. F., Jhunjhunwala, S., Kaczmarek, J. C., Hurtado, J. E., Yang, J. H., Webber, M. J., Kowalski, P. S., Heartlein, M. W., DeRosa, F., et al. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.09.006 · Cited by 191 · see citing works
Systematic discovery of structural elements governing stability of mammalian messenger RNAs
Goodarzi, H., Najafabadi, H. S., Oikonomou, P., Greco, T. M., Fish, L., Salavati, R., Cristea, I. M., & Tavazoie, S. (2012) · Nature · doi:10.1038/nature11013 · Cited by 187 · see citing works
Engineering of Therapeutic Proteins Production in Escherichia coli
Kamionka, M. (2011) · Current Pharmaceutical Biotechnology · doi:10.2174/138920111794295693 · Cited by 177 · see citing works
Zhang, M., Lin, Y.-H., Sun, Y. J., Zhu, S., Zheng, J., Liu, K., Cao, N., Li, K., Huang, Y., & Ding, S. (2016) · Cell stem cell · doi:10.1016/j.stem.2016.03.020 · Cited by 176 · see citing works
Klemke, M., Weschenfelder, T., Konstandin, M. H., & Samstag, Y. (2007) · Journal of Cellular Physiology · doi:10.1002/jcp.21029 · Cited by 167 · see citing works
Mockey, M., Gonçalves, C., Dupuy, F. P., Lemoine, F. M., Pichon, C., & Midoux, P. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.12.105 · Cited by 165 · see citing works
Colasante, G., Lignani, G., Rubio, A., Medrihan, L., Yekhlef, L., Sessa, A., Massimino, L., Giannelli, S. G., Sacchetti, S., Caiazzo, M., et al. (2015) · Cell stem cell · doi:10.1016/j.stem.2015.09.002 · Cited by 165 · see citing works
Scholten, K. B. J., Kramer, D., Taanman-Kueter, E. W., Graf, M., Schoedl, T., Meijer, C. J. L. M., Schreurs, M. W. J., & Hooijberg, E. (2006) · Clinical Immunology · doi:10.1016/j.clim.2005.12.009 · Cited by 164 · see citing works
Recombinant therapeutic proteins: Production platforms and challenges
Dingermann, T. (2007) · Biotechnology Journal · doi:10.1002/biot.200700214 · Cited by 164 · see citing works
Clinical efficacy of becaplermin (rhPDGF-BB) gel
Wieman, T. J. (1998) · The American Journal of Surgery · doi:10.1016/s0002-9610(98)00185-8 · Cited by 162 · see citing works
Embil, J. M., Papp, K. A., Sibbald, G. R., Tousignant, J., Smiell, J. M., Wong, B. Y. L., Lau, C., & THE CANADIAN BECAPLERMIN STUDY GROUP (2000) · Wound Repair and Regeneration · doi:10.1046/j.1524-475x.2000.00162.x · Cited by 161 · see citing works
Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system
DeRosa, F., Guild, B., Karve, S., Smith, L., Love, K., Dorkin, J. R., Kauffman, K. J., Zhang, J., Yahalom, B., Anderson, D. G., et al. (2016) · Gene Therapy · doi:10.1038/gt.2016.46 · Cited by 159 · see citing works
[4] Synthesis of long, capped transcripts in Vitro by SP6 and T7 RNA polymerases
Yisraeli, J. K., & Melton, D. A. (1989) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(89)80090-4 · Cited by 156 · see citing works
Effects of Chemically Modified Messenger RNA on Protein Expression
Li, B., Luo, X. L., & Dong, Y. (2016) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.6b00090 · Cited by 154 · see citing works
Phosphorothioate cap analogs stabilize mRNA and increase translational efficiency in mammalian cells
Grudzien‐Nogalska, E., Jemielity, J., Kowalska, J., Darżynkiewicz, E., & Rhoads, R. E. (2007) · RNA · doi:10.1261/rna.701307 · Cited by 152 · see citing works
Ryu, C. H., Park, S., Park, S. A., Kim, S. M., Lim, J. Y., Jeong, C. H., Yoon, W., Oh, W., Sung, Y. C., & Jeun, S. (2011) · Human Gene Therapy · doi:10.1089/hum.2010.187 · Cited by 151 · see citing works
Reverse 5' caps in RNAs made in vitro by phage RNA polymerases.
Pasquinelli, A. E., Dahlberg, J. E., & Lund, E. (1995) · PubMed · Cited by 148 · see citing works
Nakanishi, M., & Otsu, M. (2012) · Current Gene Therapy · doi:10.2174/156652312802762518 · Cited by 147 · see citing works
Li, Y., & Kiledjian, M. (2010) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.15 · Cited by 145 · see citing works
Easton, L. E., Shibata, Y., & Lukavsky, P. J. (2010) · RNA · doi:10.1261/rna.1862210 · Cited by 143 · see citing works
Nabhan, J. F., Wood, K. M., Rao, V. P., Morin, J., Bhamidipaty, S., LaBranche, T. P., Gooch, R. L., Bozal, F., Bulawa, C. E., & Guild, B. C. (2016) · Scientific Reports · doi:10.1038/srep20019 · Cited by 140 · see citing works
Function and Regulation of Retinoic Acid-Inducible Gene-I
Matsumiya, T., & Stafforini, D. M. (2010) · Critical Reviews in Immunology · doi:10.1615/critrevimmunol.v30.i6.10 · Cited by 139 · see citing works
Gallie, D. (2001) · Journal of Virology · doi:10.1128/jvi.75.24.12141-12152.2001 · Cited by 134 · see citing works
Kyriakou, C. A., Rabin, N. K., Pizzey, A. R., Nathwani, A. C., & Yong, K. (2008) · Haematologica · doi:10.3324/haematol.12553 · Cited by 130 · see citing works
Masison, D. C., Blanc, A., Ribas, J. C., Carroll, K., Sonenberg, N., & Wickner, R. B. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.5.2763 · Cited by 128 · see citing works
Ling, X., Marini, F. C., Konopleva, M. Y., Schober, W. D., Shi, Y., Burks, J. K., Clise-Dwyer, K., Wang, R., Zhang, W., Yuan, X., et al. (2010) · Cancer Microenvironment · doi:10.1007/s12307-010-0041-8 · Cited by 122 · see citing works
Protein Therapeutics for Cardiac Regeneration after Myocardial Infarction
Segers, V. F. M., & Lee, R. T. (2010) · Journal of Cardiovascular Translational Research · doi:10.1007/s12265-010-9207-5 · Cited by 121 · see citing works
Kallen, K., & Theß, A. (2013) · Therapeutic Advances in Vaccines · doi:10.1177/2051013613508729 · Cited by 120 · see citing works
Role of the SDF-1-CXCR4 axis in stem cell-based therapies for ischemic cardiomyopathy
Zaruba, M., & Franz, W. (2010) · Expert Opinion on Biological Therapy · doi:10.1517/14712590903460286 · Cited by 119 · see citing works
Akıncı, E., Banga, A., Greder, L. V., Dutton, J. R., & Slack, J. M. W. (2011) · Biochemical Journal · doi:10.1042/bj20111678 · Cited by 115 · see citing works
Youn, H., & Chung, J.-K. (2015) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2015.1057563 · Cited by 114 · see citing works
Choi, H. Y., Lee, T., Yang, G., Oh, J., Won, J., Han, J., Jeong, G., Kim, J., Kim, J., Kim, B., et al. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.06.007 · Cited by 114 · see citing works
Modified Foxp3 mRNA protects against asthma through an IL-10-dependent mechanism
Mays, L. E., Ammon-Treiber, S., Mothes, B., Alkhaled, M., Rottenberger, J., Müller-Hermelink, E. S., Grimm, M., Mezger, M., Beer-Hammer, S., von Stebut, E., et al. (2013) · Journal of Clinical Investigation · doi:10.1172/jci65351 · Cited by 113 · see citing works
RNA Mediated toll-like receptor stimulation in health and disease
Dalpke, A. H., & Helm, M. (2012) · RNA Biology · doi:10.4161/rna.20206 · Cited by 113 · see citing works
Structural and biochemical studies of the 5′→3′ exoribonuclease Xrn1
Chang, J. H., Xiang, S., Xiang, K., Manley, J. L., & Tong, L. (2011) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1984 · Cited by 113 · see citing works
Wang, Z., & Kiledjian, M. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.17.6334-6341.2000 · Cited by 113 · see citing works
Lin, C., Perche, F., Ikegami, M., Uchida, S., Kataoka, K., & Itaka, K. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.06.001 · Cited by 113 · see citing works
Cardiac regeneration: current therapies-future concepts.
Doppler, S. A., Deutsch, M.-A., Lange, R., & Krane, M. (2013) · Europe PMC (PubMed Central) · doi:10.3978/j.issn.2072-1439.2013.08.71 · Cited by 110 · see citing works
Andres, R. H., Choi, R., Pendharkar, A. V., Gaeta, X., Wang, N. N., Nathan, J. K., Chua, J. Y., Lee, S. W., Palmer, T. D., Steinberg, G. K., et al. (2011) · Stroke · doi:10.1161/strokeaha.110.606368 · Cited by 106 · see citing works
Xu, G., Jiang, X., Xu, Y., Zhang, J., Huang, F., Chen, Z., Zhou, D., Shang, J., Zou, Y., Cai, Y., et al. (2008) · Cell Biology International · doi:10.1016/j.cellbi.2008.07.023 · Cited by 105 · see citing works
A Nucleolin-Binding 3′ Untranslated Region Element Stabilizes β-Globin mRNA In Vivo
Jiang, Y., Xu, X.-S., & Russell, J. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.26.6.2419-2429.2006 · Cited by 103 · see citing works
Functions of the 5′‐terminal m7G cap in eukaryotic mRNA
Filipowicz, W. (1978) · FEBS Letters · doi:10.1016/0014-5793(78)81049-7 · Cited by 102 · see citing works
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
Summer, H., Grämer, R., & Dröge, P. (2009) · Journal of Visualized Experiments · doi:10.3791/1485 · Cited by 102 · see citing works
Liao, W., Pham, V., Liu, L., Riazifar, M., Pone, E. J., Zhang, S. X., Ma, F., Lu, M., Walsh, C. M., & Zhao, W. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.11.005 · Cited by 102 · see citing works
Lien, C.-Y., Ho, K., Lee, O. K., Blunn, G. W., & Su, Y. (2008) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.081257 · Cited by 100 · see citing works
Mueller, L. P., Luetzkendorf, J., Widder, M., Nerger, K., Caysa, H., & Mueller, T. (2010) · Cancer Gene Therapy · doi:10.1038/cgt.2010.68 · Cited by 99 · see citing works
mRNA-based gene transfer as a tool for gene and cell therapy.
Tendeloo, V. F. V., Ponsaerts, P., & Berneman, Z. N. (2007) · PubMed · Cited by 98 · see citing works
Purification and characterization of transcribed RNAs using gel filtration chromatography
McKenna, S. A., Kim, I., Puglisi, E. V., Lindhout, D. A., Aitken, C. E., Marshall, R. A., & Puglisi, J. D. (2007) · Nature Protocols · doi:10.1038/nprot.2007.480 · Cited by 98 · see citing works
Stem Cell Technology in Cardiac Regeneration: A Pluripotent Stem Cell Promise
Duelen, R., & Sampaolesi, M. (2017) · EBioMedicine · doi:10.1016/j.ebiom.2017.01.029 · Cited by 96 · see citing works
Differential Inhibition of mRNA Degradation Pathways by Novel Cap Analogs
Grudzien, E., Kalek, M., Jemielity, J., Darzynkiewicz, E., & Rhoads, R. E. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m509121200 · Cited by 90 · see citing works
Yu, J., & Russell, J. E. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.17.5879-5888.2001 · Cited by 90 · see citing works
Oligodeoxynucleotides Differentially Modulate Activation of TLR7 and TLR8 by Imidazoquinolines
Gorden, K. B., Qiu, X., Battiste, J. L., Wightman, P. D., Vasilakos, J. P., & Alkan, Ş. Ş. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.177.11.8164 · Cited by 90 · see citing works
Screening of mRNA Chemical Modification to Maximize Protein Expression with Reduced Immunogenicity
Uchida, S., Kataoka, K., & Itaka, K. (2015) · Pharmaceutics · doi:10.3390/pharmaceutics7030137 · Cited by 88 · see citing works
Moon, H.-H., Joo, M. K., Mok, H., Lee, M., Hwang, K.-C., Kim, S. W., Jeong, J. H., Choi, D., & Kim, S. H. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.019 · Cited by 84 · see citing works
Eguizábal, C., Montserrat, N., Veiga, A., & Belmonte, J. C. I. (2013) · Seminars in Reproductive Medicine · doi:10.1055/s-0032-1331802 · Cited by 83 · see citing works
Apoptosis and the cardiac action of insulin-like growth factor I.
Suleiman, M., Singh, R., & Stewart, C. (2007) · Pharmacology and Therapeutics · doi:10.1016/j.pharmthera.2007.03.001 · Cited by 82 · see citing works
Khorsand, B., Elangovan, S., Hong, L., Dewerth, A., Kormann, M., & Salem, A. K. (2017) · The AAPS Journal · doi:10.1208/s12248-016-0034-8 · Cited by 82 · see citing works
Myotonic Dystrophy: The Role of RNA CUG Triplet Repeats
Timchenko, L. (1999) · The American Journal of Human Genetics · doi:10.1086/302268 · Cited by 81 · see citing works
Khorsand, B., Nicholson, N., Do, A., Femino, J. E., Martin, J. A., Petersen, E. B., Guetschow, B., Fredericks, D. C., & Salem, A. K. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.01.008 · Cited by 81 · see citing works
Edwards, N. C., Hing, Z. A., Perry, A., Blaisdell, A., Kopelman, D., Fathke, R. L., Plum, W. G., Newell, J. M., Allen, C. E., Geetha, S., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0038864 · Cited by 80 · see citing works
Synthesis and Application of a Chain-Terminating Dinucleotide mRNA Cap Analog
Peng, Z., Sharma, V., Singleton, S. F., & Gershon, P. D. (2001) · Organic Letters · doi:10.1021/ol0167715 · Cited by 80 · see citing works
Targeted delivery of progenitor cells for cartilage repair
Dennis, J. E., Cohen, N., Goldberg, V. M., & Caplan, A. I. (2004) · Journal of Orthopaedic Research® · doi:10.1016/j.orthres.2003.12.002 · Cited by 80 · see citing works
Bontkes, H. J., Kramer, D., Ruizendaal, J. J., Taanman-Kueter, E. W., Tendeloo, V. F. V., Meijer, C. J. L. M., & Hooijberg, E. (2006) · Gene Therapy · doi:10.1038/sj.gt.3302874 · Cited by 80 · see citing works
Characterization of T‐cell immune responses of Echinococcus multilocularis‐infected C57BL/6J mice
Emery, I., Liance, M., Dériaud, E., Vuitton, D. A., Houin, R., & Leclerc, C. (1996) · Parasite Immunology · doi:10.1111/j.1365-3024.1996.tb01030.x · Cited by 80 · see citing works
Transcript-activated collagen matrix as sustained mRNA delivery system for bone regeneration
Badieyan, Z. S., Berezhanskyy, T., Utzinger, M., Aneja, M. K., Emrich, D., Erben, R. G., Schüler, C., Altpeter, P., Ferizi, M., Hasenpusch, G., et al. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.08.037 · Cited by 80 · see citing works
Virus-inspired nucleic acid delivery system: Linking virus and viral mimicry
Ni, R., Zhou, J., Hossain, N., & Chau, Y. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2016.07.005 · Cited by 78 · see citing works
Steinle, H., Behring, A., Schlensak, C., Wendel, H. P., & Avci‐Adali, M. (2016) · Stem Cells · doi:10.1002/stem.2402 · Cited by 78 · see citing works
Expression of human nephrin mRNA in diabetic nephropathy
Toyoda, M. (2004) · Nephrology Dialysis Transplantation · doi:10.1093/ndt/gfg545 · Cited by 77 · see citing works
Analysis of oligonucleotide AUG start codon context in eukariotic mRNAs
Pesole, G., Gissi, C., Grillo, G., Licciulli, F., Liuni, S., & Saccone, C. (2000) · Gene · doi:10.1016/s0378-1119(00)00471-6 · Cited by 74 · see citing works
Recent Advances in Developing Small Molecules Targeting Nucleic Acid
Wang, M., Yu, Y., Liang, C., Lü, A., & Zhang, G. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17060779 · Cited by 74 · see citing works
Vallazza, B., Petri, S., Poleganov, M. A., Eberle, F., Kuhn, A. N., & Şahin, U. (2015) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.1288 · Cited by 73 · see citing works
Synthetic Messenger RNA as a Tool for Gene Therapy
Rabinovich, P. M., Komarovskaya, M., Ye, Z.-J., Imai, C., Campana, D., Bahceci, E., & Weissman, S. (2006) · Human Gene Therapy · doi:10.1089/hum.2006.17.1027 · Cited by 73 · see citing works
Zohra, F. T., Chowdhury, E. H., Tada, S., Hoshiba, T., & Akaike, T. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.04.059 · Cited by 71 · see citing works
Al‐Saif, M., & Khabar, K. S. A. (2012) · Molecular Therapy · doi:10.1038/mt.2012.29 · Cited by 69 · see citing works
Non-viral nucleic acid delivery methods
Slivac, I., Guay, D. R. P., Mangion, M., Champeil, J., & Gaillet, B. (2016) · Expert Opinion on Biological Therapy · doi:10.1080/14712598.2017.1248941 · Cited by 68 · see citing works
Bangel‐Ruland, N., Tomczak, K., Fernández, E. F., Leier, G., Leciejewski, B., Rudolph, C., Rosenecker, J., & Weber, W. (2013) · The Journal of Gene Medicine · doi:10.1002/jgm.2748 · Cited by 67 · see citing works
Huang, C., Leblond, A., Turner, E. C., Kumar, A. H. S., Martin, K., Whelan, D., O’Sullivan, D. M., & Caplice, N. M. (2015) · Molecular Pharmaceutics · doi:10.1021/mp5006239 · Cited by 66 · see citing works
Gulck, E. R. A. V. (2005) · Blood · doi:10.1182/blood-2005-01-0339 · Cited by 63 · see citing works
Non-Viral Methods For Generating Integration-Free, Induced Pluripotent Stem Cells
Deng, X., Wang, H., Wang, T., Fang, X.-T., Zou, L., Li, Z., & Liu, C.-B. (2015) · Current Stem Cell Research & Therapy · doi:10.2174/1574888x09666140923101914 · Cited by 62 · see citing works
Balmayor, E. R., Geiger, J. P., Koch, C., Aneja, M. K., van Griensven, M., Rudolph, C., & Plank, C. (2016) · Stem Cells and Development · doi:10.1089/scd.2016.0171 · Cited by 62 · see citing works
Neural Stem Cells Differentiated From iPS Cells Spontaneously Regain Pluripotency
Choi, H. W., Kim, J. S., Choi, S., Hong, Y. J., Kim, M. J., Seo, H. G., & Tae, J. (2014) · Stem Cells · doi:10.1002/stem.1757 · Cited by 61 · see citing works
Self-citationZhi, Z., Liu, B., Jones, P. M., & Pickup, J. C. (2010) · Biomacromolecules · doi:10.1021/bm901152k · Cited by 61 · see citing works
The Pharmacology of Regenerative Medicine
Christ, G. J., Saul, J. M., Furth, M. E., & Andersson, K. (2013) · Pharmacological Reviews · doi:10.1124/pr.112.007393 · Cited by 61 · see citing works
Larouche, D., Cantin-Warren, L., Desgagné, M., Guignard, R., Martel, I., Ayoub, A., Lavoie, A., Gauvin, R., Auger, F. A., Moulin, V. J., et al. (2016) · BioResearch open access · doi:10.1089/biores.2016.0036 · Cited by 61 · see citing works
Tcherepanova, I. Y., Adams, M. D., Feng, X., Hinohara, A., Horvatinovich, J. M., Calderhead, D. M., Healey, D. G., & Nicolette, C. A. (2008) · BMC Molecular Biology · doi:10.1186/1471-2199-9-90 · Cited by 60 · see citing works
Rydzik, A. M., Kulis, M., Łukaszewicz, M., Kowalska, J., Zuberek, J., Darżynkiewicz, Z. M., Darżynkiewicz, E., & Jemielity, J. (2012) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2012.01.013 · Cited by 59 · see citing works
Preparation of Chemically Modified RNA Origami Nanostructures
Endo, M., Takeuchi, Y., Emura, T., Hidaka, K., & Sugiyama, H. (2014) · Chemistry - A European Journal · doi:10.1002/chem.201404084 · Cited by 58 · see citing works
Applications of Phosphate Modification and Labeling to Study (m)RNA Caps
Warmiński, M., Sikorski, P. J., Kowalska, J., & Jemielity, J. (2017) · Topics in Current Chemistry · doi:10.1007/s41061-017-0106-y · Cited by 57 · see citing works
Characterization of T Cell Responses to Fragrances
Sieben, S., Hertl, M., Masaoudi, T. A., Merk, H. F., & Blömeke, B. (2001) · Toxicology and Applied Pharmacology · doi:10.1006/taap.2001.9125 · Cited by 55 · see citing works
Rydzik, A. M., Łukaszewicz, M., Zuberek, J., Kowalska, J., Darżynkiewicz, Z. M., Darżynkiewicz, E., & Jemielity, J. (2009) · Organic & Biomolecular Chemistry · doi:10.1039/b911347a · Cited by 55 · see citing works
Murthy, N., Lee, K., Yu, P., Lingampalli, N., Kim, H. J., & Tang, R. (2015) · International Journal of Nanomedicine · doi:10.2147/ijn.s75124 · Cited by 53 · see citing works
Self-citationCell Replacement Strategies Aimed at Reconstitution of the β-Cell Compartment in Type 1 Diabetes
Orlando, G., Gianello, P., Salvatori, M., Stratta, R., Soker, S., Ricordi, C., & Domínguez-Bendala, J. (2014) · Diabetes · doi:10.2337/db13-1742 · Cited by 53 · see citing works
Yago, T., Leppänen, A., Carlyon, J. A., Akkoyunlu, M., Karmakar, S., Fikrig, E., Cummings, R. D., & McEver, R. P. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m305778200 · Cited by 53 · see citing works
mRNA: A Versatile Molecule for Cancer Vaccines
Diken, M., Kranz, L. M., Kreiter, S., & Şahin, U. (2016) · Current Issues in Molecular Biology · doi:10.21775/cimb.022.113 · Cited by 52 · see citing works
Kałek, M., Jemielity, J., Darżynkiewicz, Z. M., Bojarska, E., Stȩpiński, J., Stolarski, R., Davis, R. E., & Darżynkiewicz, E. (2006) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2005.12.045 · Cited by 50 · see citing works
Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression
Kim, H., Park, Y., & Lee, J. B. (2015) · Scientific Reports · doi:10.1038/srep12737 · Cited by 50 · see citing works
Antisense ribosomes: rRNA as a vehicle for antisense RNAs.
Sweeney, R., Fan, Q., & Yao, M. C. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.16.8518 · Cited by 49 · see citing works
Recent Trends in Drug Delivery System Using Protein Nanoparticles
Sripriyalakshmi, S., Jose, P., Ravindran, A., & Anjali, C. (2014) · Cell Biochemistry and Biophysics · doi:10.1007/s12013-014-9896-5 · Cited by 46 · see citing works
New strategies for improving stem cell therapy in ischemic heart disease
Huang, P., Tian, X., Li, Q., & Yang, Y. (2016) · Heart Failure Reviews · doi:10.1007/s10741-016-9576-1 · Cited by 46 · see citing works
Benteyn, D., Anguille, S., Lint, S. V., Heirman, C., Nuffel, A. M. T. V., Corthals, J., Ochsenreither, S., Waelput, W., Beneden, K. V., Breckpot, K., et al. (2013) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2013.54 · Cited by 44 · see citing works
Bohle, B., Wagner, B., Vollmann, U., Buck, D., Niggemann, B., Szépfalusi, Z., Fischer, G., Scheiner, O., Breiteneder, H., & Ebner, C. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.164.8.4393 · Cited by 43 · see citing works
Therapeutic Modulation of Growth Factors and Cytokines in Regenerative Medicine
Ioannidou, E. (2006) · Current Pharmaceutical Design · doi:10.2174/138161206777699007 · Cited by 43 · see citing works
Koubek, J., Lin, K. F., Chen, Y., Cheng, R. P., & Huang, J. J. (2013) · RNA · doi:10.1261/rna.038117.113 · Cited by 43 · see citing works
Strenkowska, M., Kowalska, J., Łukaszewicz, M., Zuberek, J., Su, W., Rhoads, R. E., Darżynkiewicz, E., & Jemielity, J. (2010) · New Journal of Chemistry · doi:10.1039/b9nj00644c · Cited by 42 · see citing works
Sridhar, A., Ohlemacher, S. K., Langer, K. B., & Meyer, J. S. (2016) · Stem Cells Translational Medicine · doi:10.5966/sctm.2015-0093 · Cited by 42 · see citing works
Cao, X., Deng, W., Qu, R., Yu, Q., Li, J., Yang, Y., Cao, Y., Gao, X., Xu, X., & Yu, J. (2013) · Advanced Functional Materials · doi:10.1002/adfm.201203646 · Cited by 40 · see citing works
Ho, Y. K., Zhou, L. H., Tam, K. C., & Too, H. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw1143 · Cited by 40 · see citing works
Flagged first, then by citations
3 of the 257 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
mRNA-based therapeutics: powerful and versatile tools to combat diseases
Qin, S., Tang, X., Chen, Y., Chen, K., Fan, N., Xiao, W., Zheng, Q., Li, G., Teng, Y., Wu, M., et al. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01007-w · Cited by 801 · see citing works
RNA-based therapeutics: an overview and prospectus
Zhu, Y., Zhu, L., Wang, X., & Jin, H. (2022) · Cell Death and Disease · doi:10.1038/s41419-022-05075-2 · Cited by 658 · see citing works
Advances in COVID-19 mRNA vaccine development
Fang, E., Liu, X., Li, M., Zhang, Z., Song, L., Zhu, B., Wu, X., Liu, J., Zhao, D., & Li, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00950-y · Cited by 601 · see citing works
mRNA vaccines manufacturing: challenges and bottlenecks
Rosa, S. S., Prazeres, D. M. F., Azevedo, A. M., & Marques, M. P. C. (2021) · Vaccine · doi:10.1016/j.vaccine.2021.03.038 · Cited by 545 · see citing works
Genetic programming of macrophages to perform anti-tumor functions using targeted mRNA nanocarriers
Zhang, F., Parayath, N. N., Ene, C., Stephan, S. B., Koehne, A. L., Coon, M. E., Holland, E. C., & Stephan, M. T. (2019) · Nature Communications · doi:10.1038/s41467-019-11911-5 · Cited by 526 · see citing works
A Comprehensive Review of mRNA Vaccines
Gote, V., Bolla, P. K., Kommineni, N., Butreddy, A., Nukala, P. K., Palakurthi, S. S., & Khan, W. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24032700 · Cited by 457 · see citing works
An Update on Self-Amplifying mRNA Vaccine Development
Blakney, A. K., Ip, S., & Geall, A. J. (2021) · Vaccines · doi:10.3390/vaccines9020097 · Cited by 288 · see citing works
Genome Editing with mRNA Encoding ZFN, TALEN, and Cas9
Zhang, H., Zhang, Y., & Yin, H. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.01.014 · Cited by 248 · see citing works
Nanomedicines to Deliver mRNA: State of the Art and Future Perspectives
Gómez-Aguado, I., Rodríguez-Castejón, J., Vicente-Pascual, M., Rodríguez‐Gascón, A., Solinís, M. Á., & del Pozo‐Rodríguez, A. (2020) · Nanomaterials · doi:10.3390/nano10020364 · Cited by 224 · see citing works
Jeong, M., Lee, Y., Park, J., Jung, H., & Lee, H. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114990 · Cited by 227 · see citing works
Self-citationImidazole‐Based Synthetic Lipidoids for In Vivo mRNA Delivery into Primary T Lymphocytes
Zhao, X., Chen, J., Qiu, M., Li, Y., Glass, Z., & Xu, Q. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.202008082 · Cited by 179 · see citing works
Recent Advancement in mRNA Vaccine Development and Applications
Fayez, N. A., Nassar, M. S., Alshehri, A. A., Alnefaie, M. K., Almughem, F. A., Alshehri, B. Y., Alawad, A. O., & Tawfik, E. A. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15071972 · Cited by 177 · see citing works
Zheng, Y., Han, Y., Sun, Q., & Li, Z. (2022) · Exploration · doi:10.1002/exp.20210166 · Cited by 151 · see citing works
mRNA vaccine in cancer therapy: Current advance and future outlook
Li, Y., Wang, M., Peng, X., Yang, Y., Chen, Q., Liu, J., She, Q., Tan, J., Lou, C., Liao, Z., et al. (2023) · Clinical and Translational Medicine · doi:10.1002/ctm2.1384 · Cited by 152 · see citing works
RNA-based scaffolds for bone regeneration: application and mechanisms of mRNA, miRNA and siRNA
Leng, Q., Chen, L., & Lv, Y. (2020) · Theranostics · doi:10.7150/thno.42640 · Cited by 146 · see citing works
Non-viral delivery of the CRISPR/Cas system: DNA versus RNA versus RNP
Lin, Y., Wagner, E., & Lächelt, U. (2022) · Biomaterials Science · doi:10.1039/d1bm01658j · Cited by 141 · see citing works
mRNA nanomedicine: Design and recent applications
Kubiatowicz, L. J., Mohapatra, A., Krishnan, N., Fang, R. H., & Zhang, L. (2022) · Exploration · doi:10.1002/exp.20210217 · Cited by 130 · see citing works
Biomedical applications of mRNA nanomedicine
Xiong, Q., Lee, G. Y., Ding, J., Li, W., & Shi, J. (2018) · Nano Research · doi:10.1007/s12274-018-2146-1 · Cited by 125 · see citing works
Revolutionizing immunization: a comprehensive review of mRNA vaccine technology and applications
Leong, K. Y., Tham, S. K., & Poh, C. L. (2025) · Virology Journal · doi:10.1186/s12985-025-02645-6 · Cited by 128 · see citing works
Machine Learning for Designing Next-Generation mRNA Therapeutics
Castillo-Hair, S. M., & Seelig, G. (2021) · Accounts of Chemical Research · doi:10.1021/acs.accounts.1c00621 · Cited by 119 · see citing works
Jeong, D., Seo, J. W., Lee, H., Jung, W. K., Park, Y. H., & Bae, H. (2022) · Advanced Science · doi:10.1002/advs.202202877 · Cited by 111 · see citing works
Self-citationOptimizing 5’UTRs for mRNA-delivered gene editing using deep learning
Castillo-Hair, S., Fedak, S., Wang, B., Linder, J., Havens, K., Certo, M., & Seelig, G. (2024) · Nature Communications · doi:10.1038/s41467-024-49508-2 · Cited by 98 · see citing works
Messenger RNA Delivery for Tissue Engineering and Regenerative Medicine Applications
Patel, S., Athirasala, A., Menezes, P. D. P., Ashwanikumar, N., Zou, T., Sahay, G., & Bertassoni, L. E. (2018) · Tissue Engineering Part A · doi:10.1089/ten.tea.2017.0444 · Cited by 99 · see citing works
Chen, Q., Zhang, Y., & Yin, H. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.10.014 · Cited by 96 · see citing works
Synthetic modified messenger RNA for therapeutic applications
Gao, M., Zhang, Q., Feng, X.-H., & Liu, J. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.06.020 · Cited by 89 · see citing works
Treatment of Hemophilia A Using Factor VIII Messenger RNA Lipid Nanoparticles
Chen, C.-Y., Tran, D. M., Cavedon, A., Cai, X., Rajendran, R., Lyle, M. J., Martini, P. G. V., & Miao, C. H. (2020) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2020.03.015 · Cited by 89 · see citing works
Future regenerative medicine developments and their therapeutic applications
Altyar, A. E., El-Sayed, A., Abdeen, A., Piscopo, M., Mousa, S. A., Najda, A., & Abdel‐Daim, M. M. (2022) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2022.114131 · Cited by 92 · see citing works
Bakhshandeh, B., Ranjbar, N., Abbasi, A., Amiri, E., Abedi, A., Mehrabi, M., Dehghani, Z., & Pennisi, C. P. (2022) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10383 · Cited by 84 · see citing works
Tumor Associated Macrophages and TAMs‐Based Anti‐Tumor Nanomedicines
Cheng, Y., Song, S., Wu, P., Lyu, B., Qin, M., Sun, Y., Sun, A., Mu, L.-M., Xu, F., Zhang, L., et al. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202100590 · Cited by 80 · see citing works
Huang, W., Guo, Q., Wu, H., Zheng, Y., Xiang, T., & Zhou, S. (2025) · ACS Nano · doi:10.1021/acsnano.5c02896 · Cited by 80 · see citing works
Developing Small Activating RNA As a Therapeutic: Current Challenges and Promises
Kwok, A., Raulf, N., & Habib, N. A. (2019) · Therapeutic Delivery · doi:10.4155/tde-2018-0061 · Cited by 78 · see citing works
mRNA-Loaded Lipid Nanoparticles Targeting Dendritic Cells for Cancer Immunotherapy
Sasaki, K., Sato, Y., Okuda, K., Iwakawa, K., & Harashima, H. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14081572 · Cited by 78 · see citing works
Zha, W., Wang, J., Guo, Z., Zhang, Y., Wang, Y., Dong, S., Liu, C., Xing, H., & Li, X. (2022) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2022.122565 · Cited by 74 · see citing works
mRNA – A game changer in regenerative medicine, cell-based therapy and reprogramming strategies
Chabanovska, O., Galow, A.-M., David, R., & Lemcke, H. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.114002 · Cited by 76 · see citing works
mRNA Therapeutic Modalities Design, Formulation and Manufacturing under Pharma 4.0 Principles
Ouranidis, A., Vavilis, T., Mandala, E., Davidopoulou, C., Stamoula, Ε., Markopoulou, C. K., Karagianni, A., & Kachrimanis, K. (2021) · Biomedicines · doi:10.3390/biomedicines10010050 · Cited by 72 · see citing works
Hydrogel Drug Delivery Systems for Bone Regeneration
Bai, L., Tao, G., Feng, M., Xie, Y., Cai, S., Peng, S., & Xiao, J. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15051334 · Cited by 70 · see citing works
Hashiba, A., Toyooka, M., Sato, Y., Maeki, M., Tokeshi, M., & Harashima, H. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.08.031 · Cited by 66 · see citing works
Adjuvant incorporated lipid nanoparticles for enhanced mRNA-mediated cancer immunotherapy
Lee, K., Kim, S. Y., Seo, Y., Kim, M. H., Chang, J., & Lee, H. (2019) · Biomaterials Science · doi:10.1039/c9bm01564g · Cited by 65 · see citing works
Self-citationEnabling mRNA Therapeutics: Current Landscape and Challenges in Manufacturing
Youssef, M., Hitti, C., Fulber, J. P. C., & Kamen, A. (2023) · Biomolecules · doi:10.3390/biom13101497 · Cited by 62 · see citing works
mRNA therapies: Pioneering a new era in rare genetic disease treatment
Shen, G., Liu, J., Yang, H., Xie, N., & Yang, Y. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2024.03.056 · Cited by 61 · see citing works
Li, C. Y., Liang, Z., Hu, Y., Zhang, H., Setiasabda, K. D., Li, J., Ma, S., Xia, X., & Kuang, Y. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.10.003 · Cited by 58 · see citing works
Messenger RNA vaccines for cancer immunotherapy: progress promotes promise
Huff, A. L., Jaffee, E. M., & Zaidi, N. (2022) · Journal of Clinical Investigation · doi:10.1172/jci156211 · Cited by 56 · see citing works
Self-amplifying mRNA vaccines: Mode of action, design, development and optimization
Pourseif, M. M., Masoudi‐Sobhanzadeh, Y., Azari, E., Parvizpour, S., Barar, J., Ansari, R. A., & Omidi, Y. (2022) · Drug Discovery Today · doi:10.1016/j.drudis.2022.103341 · Cited by 54 · see citing works
Tanaka, H., Sakurai, Y., Anindita, J., & Akita, H. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.07.001 · Cited by 56 · see citing works
Advances in mRNA therapeutics for cancer immunotherapy: From modification to delivery
Han, G., Noh, D., Lee, H., Lee, S., Kim, S., Yoon, H. Y., & Hyeon, L. S. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114973 · Cited by 52 · see citing works
Self-citationAn Update on mRNA-Based Viral Vaccines
Jeeva, S., Kim, K., Shin, C. H., Wang, B., & Kang, S. (2021) · Vaccines · doi:10.3390/vaccines9090965 · Cited by 49 · see citing works
Characterization of mRNA therapeutics
Guimaraes, G. J., Kim, J., & Bartlett, M. G. (2023) · Mass Spectrometry Reviews · doi:10.1002/mas.21856 · Cited by 48 · see citing works
Metanat, Y., Viktor, P., Amajd, A., Kaur, I., Hamed, A. M., Al-Abadi, N. K. A., Alwan, N. H., Chaitanya, M., Natrayan, L., Ghildiyal, P., et al. (2024) · Life Sciences · doi:10.1016/j.lfs.2024.122683 · Cited by 48 · see citing works
The versatile applications of polydopamine in regenerative medicine: Progress and challenges
Cai, S., Cheng, Y., Qiu, C., Liu, G., & Chu, C. (2022) · Smart Materials in Medicine · doi:10.1016/j.smaim.2022.11.005 · Cited by 46 · see citing works
Therapy Development by Genome Editing of Hematopoietic Stem Cells
Koniali, L., Lederer, C. W., & Kleanthous, M. (2021) · Cells · doi:10.3390/cells10061492 · Cited by 46 · see citing works
Engineered mRNA Delivery Systems for Biomedical Applications
Wang, J., Zhu, H., Gan, J., Liang, G., Li, L., & Zhao, Y. (2023) · Advanced Materials · doi:10.1002/adma.202308029 · Cited by 44 · see citing works
Scope and challenges of nanoparticle-based mRNA delivery in cancer treatment
Karim, M. E., Haque, S. T., Al-Busaidi, H., Bakhtiar, A., Tha, K. K., Holl, M. M. B., & Chowdhury, E. H. (2022) · Archives of Pharmacal Research · doi:10.1007/s12272-022-01418-x · Cited by 42 · see citing works
Delivery of synthetic mRNAs for tissue regeneration
Steinle, H., Weber, M., Stoppelkamp, S., Große‐Berkenbusch, K., Golombek, S., Weber, M., Çanak-Ipek, T., Trenz, S.-M., Schlensak, C., & Avci‐Adali, M. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.114007 · Cited by 41 · see citing works
In Vitro Transcribed RNA-Based Platform Vaccines: Past, Present, and Future
Perenkov, A. D., Sergeeva, A. D., Vedunova, M. V., & Krysko, D. V. (2023) · Vaccines · doi:10.3390/vaccines11101600 · Cited by 40 · see citing works
Ullah, A., Khan, M., Zhang, Y., Shafiq, M., Ullah, M., Abbas, A., Xianxiang, X., Chen, G., & Diao, Y. (2025) · International Journal of Nanomedicine · doi:10.2147/ijn.s429279 · Cited by 37 · see citing works
Cafferty, S. M., Temmerman, J. D., Kitada, T., Becraft, J. R., Weiss, R., Irvine, D. J., Devreese, M., Baere, S. D., Combes, F., & Sanders, N. N. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2020.11.010 · Cited by 37 · see citing works
Digital Twins for Continuous mRNA Production
Helgers, H., Hengelbrock, A., Schmidt, A., & Strube, J. (2021) · Processes · doi:10.3390/pr9111967 · Cited by 37 · see citing works
Microneedle systems for delivering nucleic acid drugs
Noh, I., Lee, K., & Rhee, Y.-S. (2022) · Journal of Pharmaceutical Investigation · doi:10.1007/s40005-021-00558-4 · Cited by 37 · see citing works
Self-citationZhang, J., Liu, Y., Li, C., Xiao, Q., Zhang, D., Chen, Y., Rosenecker, J., Ding, X., & Guan, S. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15092182 · Cited by 39 · see citing works
mRNA delivery via non-viral carriers for biomedical applications
Shuai, Q., Zhu, F., Zhao, M., & Yan, Y. (2021) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2021.121020 · Cited by 35 · see citing works
Amistadi, S., Maule, G., Ciciani, M., Ensinck, M. M., Keersmaecker, L. D., Ramalho, A. S., Guidone, D., Buccirossi, M., Galietta, L. J. V., Carlon, M. S., et al. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2023.03.004 · Cited by 35 · see citing works
Recent development of gene therapy for pancreatic cancer using non-viral nanovectors
Liu, Y., Wu, W., Wang, Y., Han, S., Yuan, Y., Huang, J., Shuai, X., & Peng, Z. M. (2021) · Biomaterials Science · doi:10.1039/d1bm00748c · Cited by 33 · see citing works
Opportunities and Challenges for mRNA Delivery Nanoplatforms
Zhang, Y., Hu, Y., Tian, H., & Chen, X. (2022) · The Journal of Physical Chemistry Letters · doi:10.1021/acs.jpclett.1c03898 · Cited by 33 · see citing works
Warmiński, M., Kowalska, J., Nowak, E., Kubacka, D., Tibble, R. W., Kasprzyk, R., Sikorski, P. J., Gross, J. D., Nowotny, M., & Jemielity, J. (2021) · ACS Chemical Biology · doi:10.1021/acschembio.0c00864 · Cited by 31 · see citing works
Development of mRNA Vaccines/Therapeutics and Their Delivery System
Son, S., & Lee, K. (2023) · Molecules and Cells · doi:10.14348/molcells.2023.2165 · Cited by 31 · see citing works
Self-citationPath towards mRNA delivery for cancer immunotherapy from bench to bedside
Chen, W., Zhu, Y., He, J., & Sun, X. (2023) · Theranostics · doi:10.7150/thno.89247 · Cited by 31 · see citing works
mRNA vaccines: A novel weapon to control infectious diseases
Tian, Y., Deng, Z., & Yang, P. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1008684 · Cited by 28 · see citing works
Membrane technology for the purification of RNA and DNA therapeutics
Javidanbardan, A., Messerian, K. O., & Zydney, A. L. (2024) · Trends in biotechnology · doi:10.1016/j.tibtech.2023.11.016 · Cited by 28 · see citing works
Nucleic acid delivery and nanoparticle design for COVID vaccines
Andresen, J. L., & Fenton, O. S. (2021) · MRS Bulletin · doi:10.1557/s43577-021-00169-2 · Cited by 29 · see citing works
Advances in Nonviral mRNA Delivery Materials and Their Application as Vaccines for Melanoma Therapy
Neill, B., Romero, A. R., & Fenton, O. S. (2023) · ACS Applied Bio Materials · doi:10.1021/acsabm.3c00721 · Cited by 30 · see citing works
Ige, M. A., Ren, X., Yang, Y., Zhang, H., Shen, C., Jiang, Y., Li, J., & Wan, X. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102721 · Cited by 29 · see citing works
Sato, Y. (2021) · Chemical and Pharmaceutical Bulletin · doi:10.1248/cpb.c21-00705 · Cited by 28 · see citing works
Shoop, W. K., Bacman, S. R., Barrera-Paez, J. D., & Moraes, C. T. (2023) · Nature Reviews Methods Primers · doi:10.1038/s43586-023-00200-7 · Cited by 33 · see citing works
Targeted LNPs deliver IL-15 superagonists mRNA for precision cancer therapy
Yu, J., Li, Q., Zhang, C., Wang, Q., Luo, S., Wang, X., Hu, R., & Cheng, Q. (2024) · Biomaterials · doi:10.1016/j.biomaterials.2024.123047 · Cited by 26 · see citing works
The Novelty of mRNA Viral Vaccines and Potential Harms: A Scoping Review
Halma, M. T. J., Rose, J., Jenks, A., & Lawrie, T. A. (2023) · J — Multidisciplinary Scientific Journal · doi:10.3390/j6020017 · Cited by 25 · see citing works
Advancing Cancer Immunotherapy: The Potential of mRNA Vaccines As a Promising Therapeutic Approach
Goyal, F., Chattopadhyay, A., Navik, U., Jain, A., Reddy, P. H., Bhatti, G. K., & Bhatti, J. S. (2023) · Advanced Therapeutics · doi:10.1002/adtp.202300255 · Cited by 24 · see citing works
Pandey, M., Ojha, D., Bansal, S., Rode, A. B., & Chawla, G. (2021) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2021.101003 · Cited by 23 · see citing works
Dey, C., Raina, K., Haridhasapavalan, K. K., Thool, M., Sundaravadivelu, P. K., Adhikari, P., Gogoi, R., & Thummer, R. P. (2021) · Recent Advances in iPSC Technology · doi:10.1016/b978-0-12-822231-7.00011-4 · Cited by 23 · see citing works
Puhl, D. L., Funnell, J. L., Fink, T. D., Swaminathan, A., Oudega, M., Zha, R. H., & Gilbert, R. J. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.11.025 · Cited by 23 · see citing works
mRNA delivery technologies: Toward clinical translation
Gómez-Aguado, I., Rodríguez-Castejón, J., Beraza-Millor, M., Rodríguez‐Gascón, A., del Pozo‐Rodríguez, A., & Solinís, M. Á. (2022) · International review of cytology · doi:10.1016/bs.ircmb.2022.04.010 · Cited by 23 · see citing works
Advancing mRNA vaccines for infectious diseases: key components, innovations, and clinical progress
Li, S., Zheng, L., Zhong, J., & Gao, X. (2025) · Essays in Biochemistry · doi:10.1042/ebc20253009 · Cited by 21 · see citing works
The clinical progress and challenges of mRNA vaccines
Yu, M., Wang, N., Zhu, J., & Lin, Y. (2023) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.1894 · Cited by 22 · see citing works
mRNA Vaccine Platform: mRNA Production and Delivery
Litvinova, V. R., Рудометов, А. П., Карпенко, Л. И., & Ilyichev, A. A. (2023) · Russian Journal of Bioorganic Chemistry · doi:10.1134/s1068162023020152 · Cited by 21 · see citing works
Development of mRNA vaccines and their prophylactic and therapeutic applications
Lee, K., Kim, M., Seo, Y., & Lee, H. (2018) · Nano Research · doi:10.1007/s12274-018-2095-8 · Cited by 21 · see citing works
Self-citationMichel, T., Golombek, S., Steinle, H., Hann, L., Velić, A., Maček, B., Krajewski, S., Schlensak, C., Wendel, H. P., & Avci‐Adali, M. (2019) · Journal of Biological Engineering · doi:10.1186/s13036-019-0172-5 · Cited by 20 · see citing works
The Potential of Nanomedicine to Unlock the Limitless Applications of mRNA
Taina-González, L., & de la Fuente, M. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14020460 · Cited by 21 · see citing works
RNA nanomedicine in liver diseases
Bakrania, A. K., Mo, Y., Zheng, G., & Bhat, M. (2023) · Hepatology · doi:10.1097/hep.0000000000000606 · Cited by 20 · see citing works
Leng, Q., Liang, Z., & Lv, Y. (2020) · Materials Science and Engineering C · doi:10.1016/j.msec.2020.111601 · Cited by 20 · see citing works
Unleashing the potential of mRNA therapeutics for inherited neurological diseases
Monfrini, E., Baso, G., Ronchi, D., Meneri, M., Gagliardi, D., Quetti, L., Verde, F., Ticozzi, N., Ratti, A., Fonzo, A. D., et al. (2024) · Brain · doi:10.1093/brain/awae135 · Cited by 19 · see citing works
Current Trends in Messenger RNA Technology for Cancer Therapeutics
Afzal, A., Abbasi, M. H., Ahmad, S., Sheikh, N., & Khawar, M. B. (2025) · Biomaterials Research · doi:10.34133/bmr.0178 · Cited by 21 · see citing works
Park, J. S., Jeon, H., Lee, Y., Cheon, S. Y., Lee, D., Lim, S. G., & Koo, H. (2024) · ACS Nano · doi:10.1021/acsnano.4c08939 · Cited by 19 · see citing works
Improving the Repeatability and Efficacy of Intradermal Electroporated Self-Replicating mRNA
Huysmans, H., Temmerman, J. D., Zhong, Z., Cafferty, S. M., Combes, F., Haesebrouck, F., & Sanders, N. N. (2019) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2019.06.011 · Cited by 19 · see citing works
Vibration-assisted ultrafiltration dramatically improves mRNA purification
Behboudi, A., Javidanbardan, A., Wang, Z., Zhou, L., & Zydney, A. L. (2025) · Separation and Purification Technology · doi:10.1016/j.seppur.2025.132179 · Cited by 18 · see citing works
Non-Viral RNA Therapies for Non-Small Cell Lung Cancer and Their Corresponding Clinical Trials
Tiwade, P. B., Fung, V., VanKeulen‐Miller, R., Narasipura, E. A., Ma, Y., & Fenton, O. S. (2025) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.4c00871 · Cited by 18 · see citing works
Chávez, J. C. P., McGrath, M., Kearney, C. J., Browne, S., & O’Brien, F. J. (2025) · Cell Biomaterials · doi:10.1016/j.celbio.2025.100073 · Cited by 18 · see citing works
Emerging mRNA Technology for Liver Disease Therapy
Wang, J., Fang, Y., Luo, Z., Wang, J., & Zhao, Y. (2024) · ACS Nano · doi:10.1021/acsnano.4c02987 · Cited by 19 · see citing works
Włodarczyk, J., Leng, A., Abadchi, S. N., Shababi, N., Mokhtari‐Esbuie, F., Gheshlaghi, S., Ravari, M. R., Pippenger, E. K., Afrasiabi, A., Ha, J. S., et al. (2024) · Scientific Reports · doi:10.1038/s41598-024-54941-w · Cited by 17 · see citing works
Alameh, M., & Weissman, D. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-821595-1.00014-2 · Cited by 17 · see citing works
Chen, H., Liu, Z., Yue, K., Ding, J., & He, Y. (2023) · Cell and Tissue Research · doi:10.1007/s00441-023-03743-z · Cited by 17 · see citing works
Delivery of mRNA for cancer therapy: progress and prospects
Su, L.-J., Xu, M.-X., Ji, Z.-H., Zhu, J.-Q., Yu, M., Wang, Y., & Lin, Y. (2023) · Nano Today · doi:10.1016/j.nantod.2023.102013 · Cited by 17 · see citing works
- Authors
- Kwon, H., Kim, M., Seo, Y., Moon, Y. S., Lee, H. J., Lee, K., & Lee, H.
- Affiliations
- Ewha Womans University (KR)
- Corresponding author
- Lee, Kyuri
- Lee, Hyukjin
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2018-02
- Volume
- 156
- Pages
- 172-193
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2769785887
- Cited by
- 208
- References
- 257
- Citations by year
- 2026: 17 · 2025: 40 · 2024: 32 · 2023: 43 · 2022: 30 · 2021: 19 · 2020: 14 · 2019: 9 · 2018: 4
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea
- Ministry of Science, ICT and Future Planning (2014M3C1A3054153)
- GiRC Program (2012K1A1A2A01056092)
- Basic Science Research Program (2015R1A1A1A05027352)
- BioMedical Technology Development Program (2015M3A9D7031026)
- Ministry of Education (2017R1D1A1B03028278)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- synthetic mRNA, in vitro transcription, regenerative medicine, mRNA design, cellular reprogramming, stem cell engineering, protein replacement therapy, nucleic acid therapeutics
- MeSH terms
- Animals, DNA, Humans, Plasmids, RNA, Messenger, Stem Cells, Regenerative Medicine, Cell Engineering
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review