Determining the Optimal Inhibitory Frequency for Cancerous Cells Using Tumor Treating Fields (TTFields)
Open accessAbstract
Tumor Treating Fields (TTFields) are an effective treatment modality delivered via the continuous, noninvasive application of low-intensity (1-3 V/cm), alternating electric fields in the frequency range of several hundred kHz. The study of TTFields in tissue culture is carried out using the TTFields in vitro application system, which allows for the application of electric fields of varying frequencies and intensities to ceramic Petri dishes with a high dielectric constant (Ɛ > 5,000). Cancerous cell lines plated on coverslips at the bottom of the ceramic Petri dishes are subjected to TTFields delivered in two orthogonal directions at various frequencies to facilitate treatment outcome tests, such as cell counts and clonogenic assays. The results presented in this report demonstrate that the optimal frequency of the TTFields with respect to both cell counts and clonogenic assays is 200 kHz for both ovarian and glioma cells.
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Heat energy increases with increasing frequency and reaches maximal values at frequency ranges where TTFields have optimal anti-cancer efficacy, such as 200 kHz for glioblastoma and 150 kHz for pleural malignant mesothelioma.
“Heat energy increases with increasing frequency and reaches maximal values at frequency ranges where TTFields have optimal anti-cancer efficacy (e.g., 200 kHz for glioblastoma and 150 kHz for pleural malignant mesothelioma [12,13,59]).”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [59]
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Cites
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Flagged first, then by citations
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Self-citation
- Authors
- Porat, Y., Giladi, M., Schneiderman, R. S., Blat, R., Shteingauz, A., Zeevi, E., Munster, M., Voloshin, T., Kaynan, N., Tal, O., Kirson, E. D., Weinberg, U., & Palti, Y.
- Venue
- Journal of Visualized Experiments
- Publisher
- MyJove Corporation
- Published
- 2017-05-04
- Issue
- 123
- ISSN
- 1940-087X (ISSN-L)
- OpenAlex
- W4205954044
- Cited by
- 25
- References
- 17
- Citations by year
- 2025: 3 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 4 · 2020: 4 · 2019: 3
- FWCI
- 1.13
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Visualized Experiments (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Electromagnetic Fields and Biological Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Physical Sciences › Mathematics › Modeling and Simulation › Mathematical Biology Tumor Growth
- Keywords
- tumor treating fields, electric field treatment, AC electric field, cancer cell lines, ovarian cancer cells, glioma cells, clonogenic assay, cell proliferation
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