Difficulty in obtaining the complete mRNA coding sequence at 5′ region (5′ end mRNA artifact): Causes, consequences in biology and medicine and possible solutions for obtaining the actual amino acid sequence of proteins (Review)
Open accessAbstract
The known difficulty in obtaining the actual full length, complete sequence of a messenger RNA (mRNA) may lead to the erroneous determination of its coding sequence at the 5' region (5' end mRNA artifact), and consequently to the wrong assignment of the translation start codon, leading to the inaccurate prediction of the encoded polypeptide at its amino terminus. Among the known human genes whose study was affected by this artifact, we can include disco interacting protein 2 homolog A (DIP2A; KIAA0184), Down syndrome critical region 1 (DSCR1), SON DNA binding protein (SON), trefoil factor 3 (TFF3) and URB1 ribosome biogenesis 1 homolog (URB1; KIAA0539) on chromosome 21, as well as receptor for activated C kinase 1 (RACK1, also known as GNB2L1), glutaminyl‑tRNA synthetase (QARS) and tyrosyl-DNA phosphodiesterase 2 (TDP2) along with another 474 loci, including interleukin 16 (IL16). In this review, we discuss the causes of this issue, its quantitative incidence in biomedical research, the consequences in biology and medicine, and the possible solutions for obtaining the actual amino acid sequence of proteins in the post-genomics era.
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- Authors
- Vitale, L., Caracausi, M., Casadei, R., Pelleri, M. C., & Piovesan, A.
- Affiliations
- University of Bologna (IT)
- Venue
- International Journal of Molecular Medicine
- Publisher
- Spandidos Publications
- Published
- 2017-04-06
- Volume
- 39
- Issue
- 5
- Pages
- 1063-1071
- ISSN
- 1107-3756 (ISSN-L)
- 1791-244X
- OpenAlex
- W2604121640
- Cited by
- 4
- References
- 85
- Citations by year
- 2023: 1 · 2022: 1 · 2019: 1 · 2018: 1
- FWCI
- 0.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- International Journal of Molecular Medicine (publishedVersion)
- PubMed (publishedVersion)
- Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
- Funders
- Fondazione del Monte di Bologna e Ravenna
- Università di Bologna
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- translation start codon, amino acid sequence prediction, DSCR1, sons, TFF3, RACK1, TDP2, interleukin-16, chromosome 21, ribosome biogenesis, post-genomic era
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Humans, RNA, Messenger, Open Reading Frames, Artifacts, Sequence Analysis, DNA, DNA, Complementary, Reverse Transcription
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