The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Dietary L-Arginine Supplementation Reduces White Fat Gain and Enhances Skeletal Muscle and Brown Fat Masses in Diet-Induced Obese Rats
Open accessAbstract
Previous studies showed that dietary L-arginine supplementation decreased white fat mass in genetically obese rats. This study tested the effectiveness of L-arginine in diet-induced obesity. Male Sprague-Dawley rats were fed for 15 wk a high-fat (HF) (40% energy) or low-fat (LF) (10% energy) diet beginning at 4 wk of age, resulting in 18% higher body weight gains and 74% higher weights of major white fat pads (retroperitoneal, epididymal, subcutaneous, and mesenteric adipose tissues) in HF than in LF fed rats. Starting at 19 wk of age, rats in each dietary group were supplemented for 12 wk with 1.51% L-arginine-HCl or 2.55% L-alanine (isonitrogenous control) (n = 8 per treatment) in drinking water and arginine groups were individually pair-fed to alanine controls. Despite similar energy intake, absolute weights of white fat pads increased by 98% in control rats over a 12-wk period but only by 35% in arginine-supplemented rats. The arginine treatment reduced the relative weights of white fat pads by 30% and enhanced those of soleus muscle by 13%, extensor digitorum longus muscle by 11%, and brown fat by 34% compared with control rats. Serum concentrations of insulin, adiponectin, growth hormone, corticosterone, triiodothyronine, and thyroxine did not differ between control and arginine-supplemented rats. However, arginine treatment resulted in lower serum concentrations of leptin, glucose, triglycerides, urea, glutamine, and branched-chain amino acids, higher serum concentrations of nitric-oxide metabolites, and improvement in glucose tolerance. Thus, dietary arginine supplementation shifts nutrient partitioning to promote muscle over fat gain and may provide a useful treatment for improving the metabolic profile and reducing body white fat in diet-induced obese rats.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In rats, not only has dietary L-arginine supplementation been shown to reduce fat mass, but it appears to increase skeletal muscle and brown fat mass and reduce serum concentrations of glucose, triglycerides, free fatty acids, homocysteine, dimethylarginines, and leptin as well [279, 280].”
From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [280]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
Longitudinal data analysis using generalized linear models
LIANG, K.-Y., & ZEGER, S. L. (1986) · Biometrika · doi:10.1093/biomet/73.1.13 · Cited by 18,550
Brown Adipose Tissue: Function and Physiological Significance
CANNON, B., & NEDERGAARD, J. (2004) · Physiological Reviews · doi:10.1152/physrev.00015.2003 · Cited by 6,433
Metabolism of Isolated Fat Cells
Rodbell, M. (1964) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)51687-2 · Cited by 4,647
Glutathione Metabolism and Its Implications for Health
Wu, G., Lupton, J. R., Turner, N. D., Fang, Y.-Z., & Yang, S. (2004) · The Journal of Nutrition · doi:10.1093/jn/134.3.489 · Cited by 3,842
Self-citationLinear Mixed Models for Longitudinal Data
Verbeke, G. (1997) · Lecture Notes in Statistics · doi:10.1007/978-1-4612-2294-1_3 · Cited by 3,506
Arginine metabolism: nitric oxide and beyond
WU, G., & MORRIS, S. M. (1998) · Biochemical Journal · doi:10.1042/bj3360001 · Cited by 2,811
Self-citationEnvironmental Contributions to the Obesity Epidemic
Hill, J. O., & Peters, J. C. (1998) · Science · doi:10.1126/science.280.5368.1371 · Cited by 2,258
METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.
Rodbell, M. (1964) · PubMed · Cited by 1,387
Arginine metabolism and nutrition in growth, health and disease
Wu, G., Bazer, F. W., Davis, T. A., Kim, S. W., Li, P., Marc Rhoads, J., Carey Satterfield, M., Smith, S. B., Spencer, T. E., & Yin, Y. (2009) · Amino Acids · doi:10.1007/s00726-008-0210-y · Cited by 1,362
Self-citationMitochondrial biogenesis in mammals: the role of endogenous nitric oxide
Nisoli, E., Clementi, E., Paolucci, C., Cozzi, V., Tonello, C., Sciorati, C., Bracale, R., Valerio, A., Francolini, M., Moncada, S., et al. (2003) · Science · doi:10.1126/science.1079368 · Cited by 1,243
Linear mixed models for longitudinal data
(2000) · Springer Series in Statistics · doi:10.1007/b98969 · Cited by 818
A comparison of three methods of glycogen measurement in tissues
Passonneau, J. V., & Lauderdale, V. R. (1974) · Analytical Biochemistry · doi:10.1016/0003-2697(74)90248-6 · Cited by 735
Regulatory role for the arginine–nitric oxide pathway in metabolism of energy substrates
Jobgen, W. S., Fried, S. K., Fu, W. J., Meininger, C. J., & Wu, G. (2006) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2005.12.001 · Cited by 729
Self-citationHexosamines, insulin resistance, and the complications of diabetes: current status
Buse, M. G. (2005) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00329.2005 · Cited by 528
Li, H. Z., Meininger, C. J., & Wu, G. (2000) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(00)00328-5 · Cited by 437
Self-citationEpidemiology, Trends, and Morbidities of Obesity and the Metabolic Syndrome
Bray, G. A., & Bellanger, T. (2006) · Endocrine · doi:10.1385/endo:29:1:109 · Cited by 428
Wang, J., Chen, L., Li, P., Li, X., Zhou, H., Wang, F., Li, D., Yin, Y., & Wu, G. (2008) · Journal of Nutrition · doi:10.1093/jn/138.6.1025 · Cited by 378
Self-citationA simple method to determine fat cell size and number in four mammalian species
GIROLAMO, M. D., Mendlinger, S., & Fertig, J. (1971) · American Journal of Physiology-Legacy Content · doi:10.1152/ajplegacy.1971.221.3.850 · Cited by 370
She, P., Reid, T., Bronson, S. K., Vary, T. C., Hajnal, A. S., Lynch, C. J., & Hutson, S. M. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.08.003 · Cited by 365
Dietary L-arginine supplementation reduces fat mass in Zucker diabetic fatty rats
Fu, W. J., Haynes, T. E., Kohli, R., Hu, J., Shi, W., Spencer, T. E., Carroll, R. J., Meininger, C. J., & Wu, G. (2005) · The Journal of Nutrition · doi:10.1093/jn/135.4.714 · Cited by 353
Self-citationClark, J. B., Palmer, C. J., & Shaw, W. N. (1983) · Experimental Biology and Medicine · doi:10.3181/00379727-173-41611 · Cited by 342
Tan, B., Yin, Y., Liu, Z., Xin-guo, L., Xu, H.-J., Kong, X., Huang, R., Tang, W., Shinzato, I., Smith, S. B., et al. (2008) · Amino Acids · doi:10.1007/s00726-008-0148-0 · Cited by 315
Self-citationR EGULATION OF N ITRIC O XIDE S YNTHESIS BY D IETARY F ACTORS
Wu, G., & Meininger, C. J. (2002) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.22.110901.145329 · Cited by 289
Self-citationDietary Arginine Supplementation Enhances the Growth of Milk-Fed Young Pigs
Kim, S. W., McPherson, R. L., & Wu, G. (2004) · Journal of Nutrition · doi:10.1093/jn/134.3.625 · Cited by 266
Self-citationWu, G., Collins, J. K., Perkins-Veazie, P. M., Siddiq, M., Dolan, K. D., Kelly, K. A., Heaps, C. L., & Meininger, C. J. (2007) · Journal of Nutrition · doi:10.1093/jn/137.12.2680 · Cited by 221
Self-citationMetabolomic analysis of the response of growing pigs to dietary l-arginine supplementation
He, Q., Kong, X., Wu, G., Ren, P., Tang, H., Hao, F., Huang, R., Li, T., Tan, B., Li, P., et al. (2008) · Amino Acids · doi:10.1007/s00726-008-0192-9 · Cited by 190
Self-citationAnalysis of nitrite and nitrate in biological samples using high-performance liquid chromatography
Jobgen, W. S., Jobgen, S. C., Li, H. Z., Meininger, C. J., & Wu, G. (2006) · Journal of Chromatography B · doi:10.1016/j.jchromb.2006.07.018 · Cited by 180
Self-citationAnalysis of Citrulline, Arginine, and Methylarginines Using High‐Performance Liquid Chromatography
Wu, G., & Meininger, C. J. (2008) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(07)00810-5 · Cited by 150
Self-citationRole of adipocytokines in obesity-associated insulin resistance
Zou, C., & Shao, J. (2007) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2007.06.006 · Cited by 143
Long-term high-fat feeding leads to severe insulin resistance but not diabetes in Wistar rats
Chalkley, S. M., Hettiarachchi, M., Chisholm, D. J., & Kraegen, E. W. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00173.2001 · Cited by 140
Kohli, R., Meininger, C. J., Haynes, T. E., Wene, Y. A. N., Self, J. T., & Wu, G. (2004) · Journal of Nutrition · doi:10.1093/jn/134.3.600 · Cited by 139
Self-citationRegulation of Tetrahydrobiopterin Synthesis and Bioavailability in Endothelial Cells
Shi, W., Meininger, C. J., Haynes, T. E., Hatakeyama, K., & Wu, G. (2004) · Cell Biochemistry and Biophysics · doi:10.1385/cbb:41:3:415 · Cited by 137
Self-citationSexual dimorphism in insulin sensitivity and susceptibility to develop diabetes in rats
Vital, P., Larrieta‐Carrasco, E., & Hiriart, M. (2006) · Journal of Endocrinology · doi:10.1677/joe.1.06596 · Cited by 135
Oral N-Carbamylglutamate Supplementation Increases Protein Synthesis in Skeletal Muscle of Piglets1
Frank, J. W., Escobar, J., Nguyen, H. V., Jobgen, S. C., Jobgen, W. S., Davis, T. A., & Wu, G. (2007) · Journal of Nutrition · doi:10.1093/jn/137.2.315 · Cited by 134
Self-citationWu, G., Haynes, T. E., Li, H. Z., Wene, Y. A. N., & Meininger, C. J. (2001) · Biochemical Journal · doi:10.1042/0264-6021:3530245 · Cited by 122
Self-citationWu, G., Borbolla, A. G., & Knabe, D. A. (1994) · Journal of Nutrition · doi:10.1093/jn/124.12.2437 · Cited by 121
Self-citationDietary fat and adiposity: a dose-response relationship in adult male rats fed isocalorically
Boozer, C. N., Schoenbach, G., & Atkinson, R. L. (1995) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1995.268.4.e546 · Cited by 114
Wu, G. (1995) · Diabetes · doi:10.2337/diab.44.3.360 · Cited by 106
Self-citation[24] Regulation of endothelial cell proliferation by nitric oxide
Meininger, C. J., & Wu, G. (2002) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(02)52026-7 · Cited by 60
Self-citationKowalski, T. J., Wu, G., & Watford, M. (1997) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1997.273.3.e613 · Cited by 46
Self-citationElevated Body Fat in Rats by the Dietary Nitric Oxide Synthase Inhibitor, L -NωNitroarginine
Khedara, A., Goto, T., MORISHIMA, M., Kayashita, J., & Katô, N. (1999) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.63.698 · Cited by 39
Cited by
Sorted by citations
Amino acids: Metabolism, functions, and nutrition
Wu, G. (2009) · Amino Acids · doi:10.1007/s00726-009-0269-0 · Cited by 3,004
Self-citationFunctional Amino Acids in Growth, Reproduction, and Health
Wu, G. (2010) · Advances in Nutrition · doi:10.3945/an.110.1008 · Cited by 791
Self-citationAmino Acid Nutrition in Animals: Protein Synthesis and Beyond
Wu, G., Bazer, F. W., Dai, Z., Li, D., Wang, J., & Wu, Z. (2014) · Annual Review of Animal Biosciences · doi:10.1146/annurev-animal-022513-114113 · Cited by 563
Self-citationMcKnight, J. R., Satterfield, M. C., Jobgen, W. S., Smith, S. B., Spencer, T. E., Meininger, C. J., McNeal, C. J., & Wu, G. (2010) · Amino Acids · doi:10.1007/s00726-010-0598-z · Cited by 376
Self-citationGlutamine, arginine, and leucine signaling in the intestine
Rhoads, J. M., & Wu, G. (2009) · Amino Acids · doi:10.1007/s00726-008-0225-4 · Cited by 364
Self-citation
Metadata
- Authors
- Jobgen, W., Meininger, C. J., Jobgen, S. C., Li, P., Lee, M.-J., Smith, S. B., Spencer, T. E., Fried, S. K., & Wu, G.
- Affiliations
- Texas A&M University (US)
- University of Maryland, Baltimore (US)
- Corresponding author
- Wu, Guoyao
- Venue
- The Journal of Nutrition
- Publisher
- Elsevier BV
- Published
- 2009-02
- Volume
- 139
- Issue
- 2
- Pages
- 230-237
- ISSN
- 0022-3166 (ISSN-L)
- PMC
- PMC3151442
- OpenAlex
- W2062237920
- Cited by
- 288
- References
- 42
- Citations by year
- 2026: 7 · 2025: 14 · 2024: 9 · 2023: 12 · 2022: 19 · 2021: 15 · 2020: 19 · 2019: 16 · 2018: 16 · 2017: 19 · 2016: 18 · 2015: 22 · 2014: 24 · 2013: 16 · 2012: 19
- FWCI
- 9.48
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Nutrition (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Agriculture
- American Heart Association (#0755024Y)
- Cooperative State Research, Education, and Extension Service
- National Institute of Diabetes and Digestive and Kidney Diseases (P30DK072488)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Keywords
- arginine supplementation, diet-induced obesity, white adipose tissue, brown adipose tissue, skeletal muscle mass, high-fat diet, nutrient partitioning, glucose tolerance, nitric oxide metabolites, leptin, branched-chain amino acids, glutamine, growth hormone, corticosterone, thyroxine, adiponectin, insulin
- MeSH terms
- Adipose Tissue, Amino Acids, Animals, Arginine, Blood Glucose, Body Weight, Adipose Tissue, Brown, Diet, Glucose Tolerance Test, Male, Nitric Oxide, Obesity, Organ Size, Rats, Sprague-Dawley, Muscle, Skeletal, Dietary Supplements, Leptin, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
