CD36 and SR-BI are involved in cellular uptake of provitamin A carotenoids by Caco-2 and HEK cells, and some of their genetic variants are associated with plasma concentrations of these micronutrients in humans
Open accessAbstract
Scavenger receptor class B type I (SR-BI) and cluster determinant 36 (CD36) have been involved in cellular uptake of some provitamin A carotenoids. However, data are incomplete (e.g., there are no data on α-carotene), and it is not known whether genetic variants in their encoding genes can affect provitamin A carotenoid status. The objectives were 1) to assess the involvement of these scavenger receptors in cellular uptake of the main provitamin A carotenoids (i.e., β-carotene, α-carotene, and β-cryptoxanthin) as well as that of preformed vitamin A (i.e., retinol) and 2) to investigate the contribution of genetic variations in genes encoding these proteins to interindividual variations in plasma concentrations of provitamin A carotenoids. The involvement of SR-BI and CD36 in carotenoids and retinol cellular uptake was investigated in Caco-2 and human embryonic kidney (HEK) cell lines. The involvement of scavenger receptor class B type I (SCARB1) and CD36 genetic variants on plasma concentrations of provitamin A carotenoids was assessed by association studies in 3 independent populations. Cell experiments suggested the involvement of both proteins in cellular uptake of provitamin A carotenoids but not in that of retinol. Association studies showed that several plasma provitamin A carotenoid concentrations were significantly different (P < 0.0083) between participants who bore different genotypes at single nucleotide polymorphisms and haplotypes in CD36 and SCARB1. In conclusion, SR-BI and CD36 are involved in cellular uptake of provitamin A carotenoids, and genetic variations in their encoding genes may modulate plasma concentrations of provitamin A carotenoids at a population level.
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- Authors
- Borel, P., Lietz, G., Goncalves, A., Szabo de Edelenyi, F., Lecompte, S., Curtis, P., Goumidi, L., Caslake, M. J., Miles, E. A., Packard, C., Calder, P. C., Mathers, J. C., Minihane, A. M., Tourniaire, F., Kesse-Guyot, E., Galan, P., Hercberg, S., Breidenassel, C., González Gross, M., Moussa, M., Meirhaeghe, A., & Reboul, E.
- Affiliations
- Inserm (FR)
- Newcastle University (GB)
- University of East Anglia (GB)
- University of Glasgow (GB)
- University of Southampton (GB)
- University of Bonn (DE)
- Corresponding author
- Borel, Patrick
- ORCID
- Venue
- The Journal of Nutrition
- Publisher
- Elsevier BV
- Published
- 2013-04
- Volume
- 143
- Issue
- 4
- Pages
- 448-456
- ISSN
- 0022-3166 (ISSN-L)
- OpenAlex
- W2119194983
- Cited by
- 156
- References
- 43
- Citations by year
- 2026: 13 · 2025: 9 · 2024: 13 · 2023: 16 · 2022: 15 · 2021: 13 · 2020: 15 · 2019: 14 · 2018: 13 · 2017: 14 · 2016: 4 · 2015: 4 · 2014: 10 · 2013: 3
- FWCI
- 4.63
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- ePrints Soton (University of Southampton) (submittedVersion)
- UEA Digital Repository (University of East Anglia) (submittedVersion)
- Newcastle University ePrints (Newcastle Univesity)
- Funders
- Direction Générale de la Santé (Ministry of Health)
- Médéric, Ipsen, Mutuelle Générale de l'Education Nationale, Sodexho, and Pierre Fabre
- Agence Nationale de la Recherche (ANR-05-PNRA-010)
- European Union's Sixth RTD Framework Programme (FOOD-CT-2005-007034)
- Universidad Politécnica de Madrid (CH/018/2008)
- Axis-Shield Diagnostics, Ltd. (Oslo, Norway)
- Abbot Científica S.A. (Spain)
- Ministry of Education (EX-2007-1124)
- Cognis GmbH (Germany)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Nutrition, Genetics, and Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- CD36, scavenger receptor class B type I, provitamin A carotenoids, beta-carotene, alpha-carotene, beta-cryptoxanthin, retinol uptake, genetic variants, single nucleotide polymorphisms, haplotypes, plasma carotenoid concentrations, interindividual variability, Caco-2 cells, HEK cells
- MeSH terms
- Adolescent, Carotenoids, Cross-Sectional Studies, Female, Genotype, Humans, Male, Sex Factors, Genetic Variation, Vitamin A, Caco-2 Cells, CD36 Antigens, beta Carotene, Polymorphism, Single Nucleotide, Xanthophylls, Scavenger Receptors, Class B, HEK293 Cells, Cryptoxanthins
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