Skin anti-aging and moisturizing effects of low-molecular-weight collagen peptide supplementation in healthy adults: A randomized, double-blind, placebo-controlled clinical trial
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resulting in wrinkle formation, skin dryness, and loss of elasticity [1][2][3].Collagen and hyaluronic acid (HA) are two essential macromolecules in the ECM of the skin, playing key roles in maintaining the structural integrity, hydration, and viscoelastic properties.Both the quantity and quality of these components decline with age.In particular, the degradation of collagen fibers leads to noticeable reductions in skin elasticity and density, whereas the loss of HA contributes to decreased skin moisture [2,4].Hydrolyzed collagen, or collagen peptides, is derived from the enzymatic digestion of collagen and comprises di-and tri-peptides that are efficiently absorbed into the bloodstream and distributed to the skin [5,6].These peptides can stimulate dermal fibroblasts, enhance the production of collagen and HA, and promote HA synthesis in dermal cells by upregulating the expression of hyaluronan synthase 2 [2,[7][8][9].Collagen peptides can induce structural improvements in the skin and enhance its moisture retention capacity, thereby replenishing key functional components that decline with aging and comprehensively improving overall skin health.Skin aging, a multifactorial process influenced by both intrinsic and extrinsic factors, manifests as wrinkles, dryness, reduced elasticity, and altered pore structure.Despite the promising anti-aging effects of low-molecular-weight collagen peptides (LMWCPs), heterogeneity in clinical trial designs poses limitations in fully elucidating their comprehensive efficacy.Therefore, we aimed to evaluate the efficacy and safety of collagen peptide NS (CPNS), a functional food, in improving the biophysical properties related to skin structure and moisture in healthy adults.In this randomized, doubleblind, placebo-controlled clinical trial, 70 healthy adults received either test product (1,650 mg/day, including 74.25 mg of the functional peptide, Gly-Pro) or a placebo for 8 weeks, followed by a 2-week washout.Skin parameters were measured using high-precision dermatological devices and expert visual assessments at five time points.The test group showed significant improvements in wrinkle depth, height, and visual severity scores across multiple facial regions.Skin elasticity (R2, R5, and R7), surface and deep skin hydration, as well as dermal density were markedly enhanced.Reductions in the pore number, area, depth, and volume, accompanied by decreased sebum secretion, suggested pore-tightening effects.During the 2-week discontinuation period of test product, significant effects were maintained, and throughout the study duration, no adverse events were reported.Oral administration of test product confers multifaceted benefits to skin health and represents a safe and efficacious functional ingredient to mitigate the visible signs of skin aging.
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Previous randomized controlled trials and meta-analyses have reported improvements in skin elasticity following oral or topical interventions, including collagen peptides and other bioactive compounds.
“Although previous randomized controlled trials and meta-analyses have reported improvements in skin elasticity following oral or topical interventions, including collagen peptides and other bioactive compounds [43,44,45,46,47,48]”
From A Randomized, Double-Blind, Placebo-Controlled Trial Assessing the Effects of Oral Centella asiatica Extract on Skin Aging-Related Parameters in Middle-Aged Korean Women. (2026) · cites this paper as [43]
The Results (Skin Elasticity; page unavailable) report significantly greater improvements in skin elasticity with oral collagen peptides than with placebo, and the Introduction describes earlier randomized trials with similar findings. This paper does not report a meta-analysis or findings for topical interventions or other bioactive compounds, so it supports only the oral collagen-peptide part of the cited statement. (LLM, conf=0.86)
Experimental — accuracy not yet measuredThe direction of change observed in the present study (increased skin hydration following supplementation) is generally consistent with previous reports.
“Although the direction of change observed in the present study (i.e., increased skin hydration following supplementation) is generally consistent with previous reports [43,44,45,46,47,48]”
Recent randomized controlled trials evaluating oral or topical bioactive compounds-including collagen peptides and other nutraceutical ingredients-have demonstrated improvements in wrinkle-related parameters assessed using validated quantitative techniques.
“Consistent with this, recent randomized controlled trials evaluating oral or topical bioactive compounds—including collagen peptides and other nutraceutical ingredients—have demonstrated improvements in wrinkle-related parameters assessed using validated quantitative techniques [43,44,45,46,47,48].”
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- Authors
- Lee, E., Ahn, D. K., Kim, J. H., Lee, S., Kim, H. J., Lee, H. K., & Shin, J. H.
- Affiliations
- P&K Skin Clinical Research Center, 2nd, 4th, and 5th Floors, Korea Institute of Educational Facility Safety, Seoul, Republic of Korea
- Nongshim Co., Ltd., Seoul, Republic of Korea
- Corresponding author
- Shin, Jin Hee
- ORCID
- Shin, Jin Hee
- Venue
- Journal of Microbiology and Biotechnology
- Published
- 2025-09-11
- Volume
- 35
- Pages
- e2507008-e2507008
- ISSN
- 1017-7825 (ISSN-L)
- 1738-8872
- PMC
- PMC12438954
- OpenAlex
- W4414117292
- Cited by
- 6
- References
- 20
- Citations by year
- 2026: 6
- FWCI
- 5.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Microbiology and Biotechnology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- Funders
- Nongshim Co. Ltd.
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Keywords
- skin aging, skin hydration, skin elasticity, wrinkle depth, pore size, sebum production, hyaluronic acid, dermal density, functional foods, collagen hydrolysis, healthy adults, skin biophysical properties, placebo effect, skin anti-aging, hyaluronan synthase
- MeSH terms
- Adult, Collagen, Double-Blind Method, Elasticity, Female, Humans, Male, Middle Aged, Molecular Weight, Peptides, Skin, Skin Aging, Dietary Supplements, Young Adult, Healthy Volunteers
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