Antinociceptive and anti-inflammatory effects of cellular and extracellular extracts from microalga Chlamydomonas pumilioniformis on mice
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Microalga species have attracted interest as a source of bioactive compounds with several pharmacological activities. Previous studies reported that microalgae from the genus Chlamydomonas have anti-inflammatory and antioxidant properties. In this study, antinociceptive and anti-inflammatory activities of two extracts from microalga Chlamydomonas pumilioniformis were investigated. Cellular and extracellular extracts were prepared from a 14 day-batch culture in WC medium at the end of exponential growth and their carbohydrate contents were determined. Antinociceptive effects of extracts were evaluated by writhing and formalin-induced nociception tests, while the anti-inflammatory activity was analyzed by formalin-induced paw edema in mice. The analysis of dissolved carbohydrates detected amounts of 90 and 20 µg mL-1 of total carbohydrate in cellular and extracellular extracts, respectively. Cellular extract was mainly composed of glucose, but with significant proportions of arabinose, galactose and mannose and/or xylose and minor ones of fucose, rhamnose, amino sugars and uronic acids. Extracellular extract was composed of a similar proportion of glucose, galactose and mannose/xylose, besides significant ones of arabinose, fucose and galacturonic acid. Intraperitoneal administration of extracts significantly reduced writhing response in mice. In the formalin test, the extracellular extract inhibited both formalin phases, while the cellular extract was only effective in the late phase. Furthermore, extracts reduced the formalin-induced paw edema. In sum, we showed, for the first time, that C. pumilioniformis can be an important source of polysaccharides with anti-inflammatory and antinociceptive effects.
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Phycocyanin from Spirulina and peptides from Chlorella have antinociceptive and anti-inflammatory effects in murine models of formalin-induced pain and oedema.
“phycocyanin from Spirulina and peptides from Chlorella demonstrated antinociceptive and anti-inflammatory effects in murine models of formalin-induced pain and oedema [68].”
From Microalgae Bioactives for Functional Food Innovation and Health Promotion (2025) · cites this paper as [68]
The Results and discussion report reduced formalin-induced pain behaviour and paw oedema in mice after treatment with cellular and extracellular extracts of Chlamydomonas pumilioniformis. They do not establish those effects for phycocyanin from Spirulina or peptides from Chlorella; the organisms and preparations in the claim are different. (LLM, conf=0.88)
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Antiinflammatory molecules in microalgae
Zubı́a, E., Ortega, M. J., & Reyes, C. D. L. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-22080-7.00043-0
- Authors
- Andrade, A. D., Hort, M. A., Schimith, L. E., Peraza, G. G., Maria, L. D. S., Soares, M. C. F., Giroldo, D., & Muccillo-Baisch, A. L.
- Affiliations
- Universidade Federal do Rio Grande (BR)
- Venue
- Acta Scientiarum. Biological Sciences
- Publisher
- Universidade Estadual de Maringa
- Published
- 2021-03-30
- Volume
- 43
- Pages
- e52889
- ISSN
- 1679-9283 (ISSN-L)
- 1807-863X
- OpenAlex
- W3154294377
- Cited by
- 3
- References
- 56
- Citations by year
- 2025: 2 · 2022: 1
- FWCI
- 0.18
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Acta Scientiarum Biological Sciences (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by)
- Redalyc (Universidad Autónoma del Estado de México) (submittedVersion)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ((Code 001))
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Keywords
- microalgae, antinociceptive activity, anti-inflammatory activity, polysaccharides, cell extracts, formalin-induced nociception, paw edema, carbohydrate composition, bioactive compounds
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