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Chemotaxis Under Agarose: A New and Simple Method for Measuring Chemotaxis and Spontaneous Migration of Human Polymorphonuclear Leukocytes and Monocytes
Abstract
A variety of methods have been devised for the study of spontaneous and directed cell migration. Among these, the membrane filter method introduced by Boyden in 1962, with its more recent modifications, has become the technique of choice for studies of leukocyte migration in vitro. This method, however, cannot be applied without alteration to studies of chemotaxis and spontaneous migration of cells of different types. We describe in this report a new and simple method for studying human leukocyte chemotaxis, in vitro, which is based upon migration of cells under agarose gel. This method has application to both polymorphonuclear leukocytes and monocytes, permits measurement of both chemotaxis and spontaneous migration, requires fewer cells per test, and is rapid, simple, reproducible, and inexpensive to set up.
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Citations
Cited by
A new direct-viewing chemotaxis chamber
Zicha, D., Dunn, G. A., & Brown, A. F. (1991) · Journal of Cell Science · doi:10.1242/jcs.99.4.769 · Cited by 386
Harvath, L., Falk, W., & Leonard, E. J. (1980) · Journal of Immunological Methods · doi:10.1016/0022-1759(80)90179-9 · Cited by 334
Wandall, J. H. (1992) · Gut · doi:10.1136/gut.33.5.617 · Cited by 179
Polymorphonuclear Functions and Aging in Humans*
Corberand, J., Ngyen, F., Laharrague, P., Fontanilles, A. M., Gleyzes, B., Gyrard, E., & Senegas, C. (1981) · Journal of the American Geriatrics Society · doi:10.1111/j.1532-5415.1981.tb02376.x · Cited by 143
Polymorphonuclear leukocyte dysfunction during influenza virus infection in chinchillas.
Abramson, J. S., Giebink, G. S., Mills, E. L., & Quie, P. G. (1981) · Journal of Infectious Diseases · doi:10.1093/infdis/143.6.836 · Cited by 88
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Metadata
- Authors
- Nelson, R. D., Quie, P. G., & Simmons, R. L.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1975-12-01
- Volume
- 115
- Issue
- 6
- Pages
- 1650-1656
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1819732475
- Cited by
- 1,170
- References
- 0
- FWCI
- 15.39
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Chemotaxis Under Agarose: A New and Simple Method for Measuring Chemotaxis and Spontaneous Migration of Human Polymorphonuclear Leukocytes and Monocytes. https://app.cochat.ai/papers/10.4049/jimmunol.115.6.1650
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- Nelson, R. D., Quie, P. G., & Simmons, R. L. (1975). Chemotaxis Under Agarose: A New and Simple Method for Measuring Chemotaxis and Spontaneous Migration of Human Polymorphonuclear Leukocytes and Monocytes. The Journal of Immunology. https://doi.org/10.4049/jimmunol.115.6.1650
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
