Antigen-Reactive T Cell Enrichment for Direct, High-Resolution Analysis of the Human Naive and Memory Th Cell Repertoire

Surface phenotype and antigenic specificity of human interleukin 17–producing T helper memory cells

Acosta-Rodriguez, E. V., Rivino, L., Geginat, J., Jarrossay, D., Gattorno, M., Lanzavecchia, A., Sallusto, F., & Napolitani, G. (2007) · Nature Immunology · doi:10.1038/ni1467 · Cited by 1,828

BDCA-2, BDCA-3, and BDCA-4: Three Markers for Distinct Subsets of Dendritic Cells in Human Peripheral Blood

Dzionek, A., Fuchs, A., Schmidt, P., Cremer, S., Zysk, M., Miltenyi, S., Buck, D., & Schmitz, J. (2000) · Journal of Immunology · doi:10.4049/jimmunol.165.11.6037 · Cited by 1,433

Naive CD4+ T Cell Frequency Varies for Different Epitopes and Predicts Repertoire Diversity and Response Magnitude

Moon, J. J., Chu, H., Pepper, M., McSorley, S. J., Jameson, S. C., Kedl, R. M., & Jenkins, M. K. (2007) · Immunity · doi:10.1016/j.immuni.2007.07.007 · Cited by 977

Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β

Zielinski, C. E., Mele, F., Aschenbrenner, D., Jarrossay, D., Ronchi, F., Gattorno, M., Monticelli, S., Lanzavecchia, A., & Sallusto, F. (2012) · Nature · doi:10.1038/nature10957 · Cited by 919

Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8+ T cell phenotypes

Newell, E. W., Sigal, N., Bendall, S. C., Nolan, G. P., & Davis, M. M. (2012) · Immunity · doi:10.1016/j.immuni.2012.01.002 · Cited by 555

Pre-B-cell colony-enhancing factor, whose expression is up-regulated in activated lymphocytes, is a nicotinamide phosphoribosyltransferase, a cytosolic enzyme involved in NAD biosynthesis

Rongvaux, A., Shea, R. J., Mulks, M. H., Gigot, D., Urbain, J., Léo, O., & Andris, F. (2002) · European Journal of Immunology · doi:10.1002/1521-4141(200211)32:11 · Cited by 551

Direct access to CD4+ T cells specific for defined antigens according to CD154 expression

Frentsch, M., Arbach, O., Kirchhoff, D., Moewes, B., Worm, M., Rothe, M., Scheffold, A., & Thiel, A. (2005) · Nature Medicine · doi:10.1038/nm1292 · Cited by 475

Activation-induced expression of CD137 permits detection, isolation, and expansion of the full repertoire of CD8+ T cells responding to antigen without requiring knowledge of epitope specificities

Wölfl, M., Kuball, J. H. E., Ho, W., Nguyen, H., Manley, T. J., Bleakley, M., & Greenberg, P. D. (2007) · Blood · doi:10.1182/blood-2006-11-056168 · Cited by 450

Endogenous naive CD8+ T cell precursor frequency regulates primary and memory responses to infection

Obar, J. J., Khanna, K. M., & Lefrançois, L. (2008) · Immunity · doi:10.1016/j.immuni.2008.04.010 · Cited by 449

MHC class II tetramers identify peptide-specific human CD4+ T cells proliferating in response to influenza A antigen

Novak, E. J., Liu, A. W., Nepom, G. T., & Kwok, W. W. (1999) · Journal of Clinical Investigation · doi:10.1172/jci8476 · Cited by 376

A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles

Chattopadhyay, P. K., Yu, J., & Roederer, M. (2005) · Nature Medicine · doi:10.1038/nm1293 · Cited by 321

The Role of Naive T Cell Precursor Frequency and Recruitment in Dictating Immune Response Magnitude

Jenkins, M. K., & Moon, J. J. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1102661 · Cited by 317

Ex vivo analysis of human memory CD4 T cells specific for hepatitis C virus using MHC class II tetramers

Day, C. L., Seth, N. P., Lucas, M., Appel, H., Gauthier, L., Lauer, G. M., Robbins, G. K., Szczepiorkowski, Z. M., Casson, D. R., Chung, R. T., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci18509 · Cited by 294

On the Composition of the Preimmune Repertoire of T Cells Specific for Peptide–Major Histocompatibility Complex Ligands

Jenkins, M. K., Chu, H., McLachlan, J. B., & Moon, J. J. (2010) · Annual Review of Immunology · doi:10.1146/annurev-immunol-030409-101253 · Cited by 256

Development of a homologous transformation system for the human pathogenic fungus Aspergillus fumigatus based on the pyrG gene encoding orotidine 5′′-monophosphate decarboxylase

Weidner, G., d’Enfert, C., Koch, A., Mol, P. C., & Brakhage, A. A. (1998) · Current Genetics · doi:10.1007/s002940050350 · Cited by 213

Human naive and memory CD4+ T cell repertoires specific for naturally processed antigens analyzed using libraries of amplified T cells

Geiger, R., Duhen, T. M., Lanzavecchia, A., & Sallusto, F. (2009) · The Journal of Experimental Medicine · doi:10.1084/jem.20090504 · Cited by 212

Enumeration of human antigen–specific naive CD8+ T cells reveals conserved precursor frequencies

Alanio, C., Lemaı̂tre, F., Law, H., Hasan, M., & Albert, M. L. (2010) · Blood · doi:10.1182/blood-2009-10-251124 · Cited by 179

A sensitive method for detecting proliferation of rare autoantigen-specific human T cells

Mannering, S. I., Morris, J. S., Jensen, K. P., Purcell, A. W., Honeyman, M. C., Endert, P. V., & Harrison, L. C. (2003) · Journal of Immunological Methods · doi:10.1016/j.jim.2003.09.004 · Cited by 175

In vitro methods for generating CD8+ T-cell clones for immunotherapy from the naïve repertoire

Ho, W., Nguyen, H., Wölfl, M., Kuball, J. H. E., & Greenberg, P. D. (2006) · Journal of Immunological Methods · doi:10.1016/j.jim.2005.11.023 · Cited by 120

Frequency of Epitope-Specific Naive CD4+ T Cells Correlates with Immunodominance in the Human Memory Repertoire

Kwok, W. W., Tan, V., Gillette, L., Littell, C. T., Soltis, M. A., LaFond, R. E., Yang, J., James, E. A., & DeLong, J. H. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1102190 · Cited by 120

MHC Class II Tetramers

Nepom, G. T. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1102398 · Cited by 118

Measurement ofCandida-specific blastogenesis: Comparison of carboxyfluorescein succinimidyl ester labelling of T cells, thymidine incorporation, and CD69 expression

Angulo, R., & Fulcher, D. A. (1998) · Cytometry · doi:10.1002/(sici)1097-0320(19980615)34:3 · Cited by 94

Ultrasensitive Detection and Phenotyping of CD4+ T Cells with Optimized HLA Class II Tetramer Staining

Scriba, T. J., Purbhoo, M. A., Day, C. L., Robinson, N., Fidler, S. J., Fox, J., Weber, J. N., Klenerman, P., Sewell, A. K., & Phillips, R. E. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.10.6334 · Cited by 92

Characterization of CD4+ T cell subsets in allergy

Wambre, E. R., James, E. A., & Kwok, W. W. (2012) · Current Opinion in Immunology · doi:10.1016/j.coi.2012.07.009 · Cited by 81

Flow cytometric analysis of vaccine responses: how many colors are enough?

Roederer, M., Brenchley, J. M., Betts, M. R., & Rosa, S. C. D. (2004) · Clinical Immunology · doi:10.1016/j.clim.2003.11.015 · Cited by 76

Mapping T cell epitopes by flow cytometry

Hoffmeister, B. (2003) · Methods · doi:10.1016/s1046-2023(02)00349-3 · Cited by 74

Flow Cytometry

Macey, M. G. (2007) · Humana Press eBooks · doi:10.1007/978-1-59745-451-3 · Cited by 73

Identification and isolation of murine antigen‐reactive T cells according to CD154 expression

Kirchhoff, D., Frentsch, M., Leclerk, P., Bumann, D., Rausch, S., Hartmann, S., Thiel, A., & Scheffold, A. (2007) · European Journal of Immunology · doi:10.1002/eji.200737322 · Cited by 65

Ultra‐sensitive class I tetramer analysis reveals previously undetectable populations of antiviral CD8+ T cells

Barnes, E. J., Ward, S. M., Kasprowicz, V. O., Dusheiko, G. M., Klenerman, P., & Lucas, M. (2004) · European Journal of Immunology · doi:10.1002/eji.200424898 · Cited by 58

Positive selection optimizes the number and function of MHCII-restricted CD4 + T cell clones in the naive polyclonal repertoire

Chu, H., Moon, J. J., Takada, K., Pepper, M., Molitor, J. A., Schacker, T. W., Hogquist, K. A., Jameson, S. C., & Jenkins, M. K. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0902015106 · Cited by 51

Multiparameter Flow Cytometry Monitoring of T Cell Responses

Maecker, H. T. (2009) · Methods in molecular biology · doi:10.1007/978-1-59745-170-3_25 · Cited by 46

Negative Selection and Peptide Chemistry Determine the Size of Naive Foreign Peptide–MHC Class II-Specific CD4+ T Cell Populations

Chu, H., Moon, J. J., Kruse, A. C., Pepper, M., & Jenkins, M. K. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.1002276 · Cited by 43

Antigen-specific cytometry—New tools arrived!

Thiel, A., Scheffold, A., & Radbruch, A. (2004) · Clinical Immunology · doi:10.1016/j.clim.2003.12.017 · Cited by 41

Flow cytometry and the future of vaccine development

Bolton, D. L., & Roederer, M. (2009) · Expert Review of Vaccines · doi:10.1586/erv.09.41 · Cited by 40

Simultaneous Cytometric Analysis of (Auto)antigen-Reactive T and B Cell Proliferation

Schneider, S. M. (2002) · Immunobiology · doi:10.1078/0171-2985-00196 · Cited by 23

Recent Developments in Flow Cytometry

Scheffold, A., & Kern, F. (2000) · Journal of Clinical Immunology · doi:10.1023/a:1026454225515 · Cited by 20

Untitled work

Rodriguez-Palmero, M. (1999) · doi:10.1002/(sici)1521-4141(199912)29:12

Identification of preexisting adaptive immunity to Cas9 proteins in humans

Charlesworth, C. T., Deshpande, P. S., Dever, D. P., Camarena, J., Lemgart, V. T., Cromer, M. K., Vakulskas, C. A., Collingwood, M. A., Zhang, L., Bode, N. M., et al. (2019) · Nature Medicine · doi:10.1038/s41591-018-0326-x · Cited by 1,093

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Cossarizza, A., Chang, H., Radbruch, A., Acs, A., Adam, D., Adam‐Klages, S., Agace, W. W., Aghaeepour, N., Akdiş, M., Allez, M., et al. (2019) · European Journal of Immunology · doi:10.1002/eji.201970107 · Cited by 1,007

Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4+ T Cells in COVID-19

Meckiff, B. J., Ramírez-Suástegui, C., Fajardo, V., Chee, S., Kusnadi, A., Simon, H. A., Eschweiler, S., Grifoni, A., Pelosi, E., Weiskopf, D., et al. (2020) · Cell · doi:10.1016/j.cell.2020.10.001 · Cited by 581

Guidelines for the use of flow cytometry and cell sorting in immunological studies *

Cossarizza, A., Chang, H., Radbruch, A., Akdiş, M., Andrä, I., Annunziato, F., Bächer, P., Barnaba, V., Battistini, L., Bauer, W. M., et al. (2017) · European Journal of Immunology · doi:10.1002/eji.201646632 · Cited by 595

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza, A., Chang, H., Radbruch, A., Abrignani, S., Addo, R., Akdis, M., Andrä, I., Andreata, F., Annunziato, F., Arranz, E., et al. (2021) · European Journal of Immunology · doi:10.1002/eji.202170126 · Cited by 531

A high-sensitivity detection system for monitoring of rare antigen specific naive and memory T helper cells (58.6)

Bächer, P., Schink, C., Teutschbein, J., Kniemeyer, O., Assenmacher, M., Brakhage, A. A., Schmitz, J., & Scheffold, A. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.188.supp.58.6

Flow Cytometric Characterization of Human Antigen-Reactive T-Helper Cells

Saggau, C., Scheffold, A., & Bächer, P. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1311-5_12 · Cited by 16

Direct access to CD4+ T cells specific for defined antigens according to CD154 expression

Frentsch, M., Arbach, O., Kirchhoff, D., Moewes, B., Worm, M., Rothe, M., Scheffold, A., & Thiel, A. (2005) · Nature Medicine · doi:10.1038/nm1292 · Cited by 475

Flow-cytometric analysis of rare antigen-specific T cells

Bacher, P., & Scheffold, A. (2013) · Cytometry Part A · doi:10.1002/cyto.a.22317 · Cited by 155

On-Chip Activation and Subsequent Detection of Individual Antigen-Specific T Cells

Song, Q., Han, Q., Bradshaw, E. M., Kent, S. C., Raddassi, K., Nilsson, B., Nepom, G. T., Hafler, D. A., & Love, J. C. (2009) · Analytical Chemistry · doi:10.1021/ac9024363 · Cited by 31