TFEC is a macrophage-restricted member of the microphthalmia-TFE subfamily of basic helix-loop-helix leucine zipper transcription factors
Abstract
The murine homologue of the TFEC was cloned as part of an analysis of the expression of the microphthalmia-TFE (MiT) subfamily of transcription factors in macrophages. TFEC, which most likely acts as a transcriptional repressor in heterodimers with other MiT family members, was identified in cells of the mononuclear phagocyte lineage, coexpressed with all other known MiT subfamily members (Mitf, TFE3, TFEB). Northern blot analysis of several different cell lineages indicated that the expression of murine TFEC (mTFEC) was restricted to macrophages. A 600-bp fragment of the TATA-less putative proximal promoter of TFEC shares features with many known macrophage-specific promoters and preferentially directs luciferase expression in the RAW264.7 macrophage cell line in transient transfection assays. Five of six putative Ets motifs identified in the TFEC promoter bind the macrophage-restricted transcription factor PU.1 under in vitro conditions and in transfected 3T3 fibroblasts; the minimal luciferase activity of the TFEC promoter could be induced by coexpression of PU.1 or the related transcription factor Ets-2. The functional importance of the tissue-restricted expression of TFEC and a possible role in macrophage-specific gene regulation require further investigation, but are likely to be linked to the role of the other MiT family members in this lineage.
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In mouse, tfec transcription is activated through multiple ets-binding domains in its promoter region.
“In mouse, tfec transcription is activated through multiple ets-binding domains in its promoter region”
A conserved regulatory mechanism for ETS1A in tfec transcriptional regulation is suggested.
“suggesting a conserved regulatory mechanism for ETS1A in tfec transcriptional regulation as well [84].”
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- Authors
- Rehli, M., Lichanska, A., Cassady, A. I., Ostrowski, M. C., & Hume, D. A.
- Affiliations
- The University of Queensland (AU)
- The Ohio State University (US)
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-02-01
- Volume
- 162
- Issue
- 3
- Pages
- 1559-1565
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2101675954
- Cited by
- 116
- References
- 8
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 7 · 2020: 8 · 2019: 5 · 2018: 6 · 2017: 4 · 2016: 4 · 2015: 2 · 2014: 5 · 2013: 1 · 2012: 4
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- TFEC, macrophages, transcriptional repressor, heterodimerization, MITF, TFE3, TFEB, promoter analysis, PU.1, ETS2, RAW 264.7 macrophages
- MeSH terms
- Proto-Oncogene Protein Spi-1, Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cloning, Molecular, DNA-Binding Proteins, Macrophages, Microphthalmos, Molecular Sequence Data, Osteoclasts, Promoter Regions, Genetic, Proto-Oncogene Proteins, Sequence Homology, Nucleic Acid, Transcription Factors, Transcriptional Activation, Trans-Activators, Leucine Zippers, DNA, Complementary, Helix-Loop-Helix Motifs, Mice, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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