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Induction of Th2 Responses and IgE Is Largely Due to Carbohydrates Functioning as Adjuvants on Schistosoma mansoni Egg Antigens
Abstract
Infection with the helminth parasite Schistosoma mansoni induces a pronounced Th2-type response that is associated with significant IgE production. To better understand how the parasite drives these responses, we investigated the relative roles of proteins and carbohydrates in driving Th2-type and/or IgE responses using a murine model of intranasal sensitization with soluble egg Ags (SEA) of Schistosoma mansoni. We found that repeated intranasal sensitization with soluble egg Ags led to the induction of both total and specific IgE production and nasal eosinophilia. By comparing the responses of mice sensitized with SEA or metaperiodate-treated SEA we were able to demonstrate that carbohydrates on SEA are the major inducers of IgE production and nasal recruitment of eosinophils. Mice sensitized with periodate-treated SEA displayed a significant decrease in both total and specific IgE levels in comparison to mice sensitized with native SEA. Furthermore, sensitization of mice with periodate-treated SEA significantly reduced levels of Ag-specific IgG1, but had no effect on IgG2a production. Nasal lymphocytes from mice sensitized with native SEA, but not with periodate-treated SEA, produced IL-4, IL-5, and IL-10 when restimulated with native SEA in vitro. On the other hand, lymphocytes from mice sensitized with periodate-treated SEA did not produce any of these same cytokines following in vitro restimulation, suggesting that carbohydrates were required for in vivo induction of Th2 response and for that of associated cytokine responses in this model. Lastly, competitive inhibition ELISA showed that although carbohydrates are required for SEA-specific IgE induction, they are not targets of the induced IgE response.
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“S. mansoni soluble egg antigen (SEA) induces Th2 cell responses in vivo in a glycan-dependent manner”
From Modulation of Host Immunity by Helminths: The Expanding Repertoire of Parasite Effector Molecules (2018) · cites this paper as Okano et al., 1999
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Jones, M. K., Candido, R. R. F., Pierre, T. G. S., Woodward, R., Kusel, J. R., & Teixeira, C. (2016) · CRC Press eBooks · doi:10.1201/9781315368900-6
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ACTIVATION OF MACROPHAGES IN VIVO BY GLYCAN LNFPIII
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Functional genomics methods to target the interface between schistosomes and the host immune system
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Metadata
- Authors
- Okano, M., Satoskar, A. R., Nishizaki, K., Abe, M., & Harn, D. A.
- Affiliations
- Harvard University (US)
- Okayama University (JP)
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-12
- Volume
- 163
- Issue
- 12
- Pages
- 6712-6717
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2097456894
- Cited by
- 206
- References
- 32
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 6 · 2022: 1 · 2021: 6 · 2020: 3 · 2019: 6 · 2018: 6 · 2017: 4 · 2016: 5 · 2015: 6 · 2014: 10 · 2013: 4 · 2012: 13
- FWCI
- 7.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI-16305-18)
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Asthma and respiratory diseases
- Keywords
- Schistosoma mansoni, soluble egg antigen, Th2 immune response, eosinophilia, periodate treatment, intranasal sensitization, IL-4, IL-5, IL-10, IgG1, IgG2a, helminthiasis
- MeSH terms
- Adjuvants, Immunologic, Administration, Intranasal, Animals, Antibodies, Helminth, Antigens, Helminth, Carbohydrates, Cells, Cultured, Eosinophilia, Female, Immunoglobulin E, Mice, Inbred BALB C, Nasal Mucosa, Ovum, Periodic Acid, Schistosoma mansoni, Cytokines, Th2 Cells, Epitopes, T-Lymphocyte, Carbohydrate Metabolism, Mice
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