The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Quantifying the Frequency of Alloreactive T Cells In Vivo: New Answers to an Old Question
Open accessAbstract
Alloreactive T cell precursor frequency was measured in vivo using fluorescent dye labeling in combination with novel models based on lymphocyte activation and recovery. CFSE-labeled C57BL/6 (H-2b) spleen and lymph node cells were adoptively transferred to C57BL/6×DBA F1 (H-2b/d) recipients, a parent→F1 MHC mismatch in which only donor cells respond. Recipients were sacrificed at serial time points to assess engraftment efficiency, and the extent of donor cell activation and proliferation. These data were used to calculate alloreactive T cell frequencies that varied 30-fold (0.71 ± 0.31% to 21.05 ± 3.62%), depending upon whether it was assumed that all donor cells injected became established and were capable of responding, or that only those present at later time points (24–72 h) were available to respond. By measuring the number of cells established in the recipient 24 h after transfer, before proliferation, we calculated an in vivo alloreactive frequency of ∼7%. Using CD69 expression at 48 h to quantify activation, we found that 40–50% of the alloactivated CD4+ donor T cells do not divide. Studies of cotransferred congenic and allogeneic cells demonstrated that bystander proliferation does not occur. We conclude that accurate calculations of alloreactive precursor frequency must account for both proliferation and cell engraftment. When this is done, a high percentage of alloreactive T cells exists across an MHC mismatch, but not all alloreactive cells proliferate in vivo. Bystander proliferation is negligible, revealing exquisite specificity to the alloresponse. These data provide a novel approach to quantify alloreactive T cell responses during specific immunomodulatory strategies in vivo.
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Helper T Cell Differentiation Is Controlled by the Cell Cycle
Bird, J. J., Brown, D. R., Mullen, A. C., Moskowitz, N. H., Mahowald, M. A., Sider, J. R., Gajewski, T. F., Wang, C.-R., & Reiner, S. L. (1998) · Immunity · doi:10.1016/s1074-7613(00)80605-6 · Cited by 832
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Self-citationRequirement for T-cell apoptosis in the induction of peripheral transplantation tolerance
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Self-citationWhy do so many lymphocytes respond to major histocompatibility antigens?
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The Molecular Basis of Allorecognition
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Allo-HLA reactivity of virus-specific memory T cells is common
Amir, A. L., D'Orsogna, L. J. A., Roelen, D. L., van Loenen, M. M., Hagedoorn, R. S., de Boer, R., van der Hoorn, M. A. W. G., Kester, M. G. D., Doxiadis, I. I. N., Falkenburg, J. H. F., et al. (2010) · Blood · doi:10.1182/blood-2009-07-234906 · Cited by 294
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Metadata
- Authors
- Suchin, E. J., Langmuir, P. B., Palmer, E., Sayegh, M. H., Wells, A. D., & Turka, L. A.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-01
- Volume
- 166
- Issue
- 2
- Pages
- 973-981
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1630218117
- Cited by
- 517
- References
- 17
- FWCI
- 9.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Suchin, E. J., Langmuir, P. B., Palmer, E., Sayegh, M. H., Wells, A. D., & Turka, L. A. (2001). Quantifying the Frequency of Alloreactive T Cells In Vivo: New Answers to an Old Question. The Journal of Immunology. https://doi.org/10.4049/jimmunol.166.2.973
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