The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Primary Tumor Tissue Lysates Are Enriched in Heat Shock Proteins and Induce the Maturation of Human Dendritic Cells
Abstract
Upon exposure to lysates or supernatants of necrotic transformed cell lines, human dendritic cells (DCs) undergo maturation. In contrast, DCs exposed to apoptotic transformed cell lines or necrotic lysates of primary cells remain immature. Analysis of supernatants of necrotic transformed cell lines showed them to be enriched in the heat shock proteins (hsp)70 and gp96, in contrast to supernatants of primary cells. Likewise, cells from a variety of primary human tumors contained considerably higher levels of hsp than their normal autologous tissue counterparts. Of the majority of human tumors enriched in hsps (hsp70 and/or gp96), their corresponding lysates matured DCs. The maturation effect of tumor cell lysates was abrogated by treatment with boiling, proteinase K, and geldanamycin, an inhibitor of hsps, suggesting that hsps rather than endotoxin or DNA were the responsible factors. Supporting this idea, highly purified, endotoxin-depleted hsp70, induced DC maturation similar to that seen with standard maturation stimuli LPS and monocyte conditioned medium. These results suggest that the maturation activity inherent within tumor cells and lines is mediated at least in part by hsps. The release of hsps in vivo as a result of cell injury should promote immunity through the maturation of resident DCs.
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Cites
Dendritic cells and the control of immunity
Banchereau, J., & Steinman, R. M. (1998) · Nature · doi:10.1038/32588 · Cited by 14,336
Cella, M., Scheidegger, D., Palmer-Lehmann, K., Lane, P., Lanzavecchia, A., & Alber, G. (1996) · The Journal of experimental medicine · doi:10.1084/jem.184.2.747 · Cited by 1,983
Natural adjuvants: Endogenous activators of dendritic cells
Gallucci, S., Lolkema, M., & Matzinger, P. (1999) · Nature Medicine · doi:10.1038/15200 · Cited by 1,602
Sauter, B., Albert, M. L., Francisco, L., Larsson, M., Somersan, S., & Bhardwaj, N. (2000) · The Journal of Experimental Medicine · doi:10.1084/jem.191.3.423 · Cited by 1,358
Self-citationOrigin, maturation and antigen presenting function of dendritic cells
Cella, M., Sallusto, F., & Lanzavecchia, A. (1997) · Current Opinion in Immunology · doi:10.1016/s0952-7915(97)80153-7 · Cited by 1,320
Activation of human dendritic cells through CD40 cross-linking.
Caux, C., Massacrier, C., Vanbervliet, B., Dubois, B., Van Kooten, C., Durand, I., & Banchereau, J. (1994) · The Journal of experimental medicine · doi:10.1084/jem.180.4.1263 · Cited by 1,317
Basu, S., Binder, R. J., Suto, R., Anderson, K. M., & Srivastava, P. K. (2000) · International Immunology · doi:10.1093/intimm/12.11.1539 · Cited by 1,305
Self-citationRomani, N., Reider, D., Heuer, M., Ebner, S., Kämpgen, E., Eibl, B., Niederwieser, D., & Schuler, G. (1996) · Journal of Immunological Methods · doi:10.1016/0022-1759(96)00078-6 · Cited by 1,091
CD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells.
Zhou, L. J., & Tedder, T. F. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.6.2588 · Cited by 1,067
Roe, S. M., Prodromou, C., O'Brien, R., Ladbury, J. E., Piper, P. W., & Pearl, L. H. (1999) · Journal of Medicinal Chemistry · doi:10.1021/jm980403y · Cited by 1,004
Cited by
Immunological aspects of cancer chemotherapy
Zitvogel, L., Apetoh, L., Ghiringhelli, F., & Kroemer, G. (2008) · Nature Reviews Immunology · doi:10.1038/nri2216 · Cited by 1,712
Roles of heat-shock proteins in innate and adaptive immunity.
Srivastava, P. (2002) · Nature reviews. Immunology · doi:10.1038/nri749 · Cited by 1,069
Self-citationSrivastava, P. (2002) · Annual Review of Immunology · doi:10.1146/annurev.immunol.20.100301.064801 · Cited by 850
Self-citationThe CD8+ Dendritic Cell Subset Selectively Endocytoses Dying Cells in Culture and In Vivo
Iyoda, T., Shimoyama, S., Liu, K., Omatsu, Y., Akiyama, Y., Maeda, Y., Takahara, K., Steinman, R. M., & Inaba, K. (2002) · The Journal of Experimental Medicine · doi:10.1084/jem.20020161 · Cited by 648
Cross‐presentation: underlying mechanisms and role in immune surveillance
Rock, K. L., & Shen, L. (2005) · Immunological Reviews · doi:10.1111/j.0105-2896.2005.00301.x · Cited by 451
Metadata
- Authors
- Somersan, S., Larsson, M., Fonteneau, J. F., Basu, S., Srivastava, P., & Bhardwaj, N.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-11-01
- Volume
- 167
- Issue
- 9
- Pages
- 4844-4852
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1736132406
- Cited by
- 258
- References
- 49
- FWCI
- 8.33
- Type
- journal article
- Language
- en
- Open access
- closed
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- Somersan, S., Larsson, M., Fonteneau, J. F., Basu, S., Srivastava, P., & Bhardwaj, N. (2001). Primary Tumor Tissue Lysates Are Enriched in Heat Shock Proteins and Induce the Maturation of Human Dendritic Cells. The Journal of Immunology. https://doi.org/10.4049/jimmunol.167.9.4844
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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